Mp78-05 Invasion-Associated Mirnas S as Possible Diagnostic Biomarkers of Muscle Invasive Bladder Cancer in Tumor Tissues and Urinary Exosomes

Abstract

myeloid-derived suppressor cells (MDSC) could be affected by chemotherapy and correlate with pathologic response in bladder cancer patients receiving neo-adjuvant chemotherapy.METHODS: Pretreatment Peripheral blood levels of granulocytic (G-MDSC) and monocytic MDSC (M-MDSC) were measured by fow cytometry. We divided patients into high and low (above and below the median, respectively) groups based on the median value for each immune cell subset and compared outcomes of the two groups.RESULTS: A signi?cant pathological response (pT0-1) was attained in 14% (3 of 22) of patients with low G-MDSC compared with 60% (13 of 22) of patients with high G-MDSC (P¼0.042). Patients with high G-MDSC had signi?cantly shorter diseaseespecific survival and progression-free survival (both P<0.001). In the multivariate analysis for survival, only high G-MSDC levels for progression-free survival (P¼0.022), high G-MDSC and pathological response emerged as independent prognostic factor for diseasespecific survival(P¼0.004 and 0.040 respectively).CONCLUSIONS: These results provide evidence that pretreatment peripheral G-MDSCs can inform the outcome of patients treated with platinum-based neoadjuvant chemotherapy

Role of Exosomal miRNA in Bladder Cancer: A Promising Liquid Biopsy Biomarker

“…Urinary biomarkers could also be used to correctly identify MIBC patients that require radical cystectomy, a treatment that severely affects the quality of life. Although no such biomarkers have been identified to date, Baumgart et al identified three miRNAs (miR-30a-3p, miR-99a-5p, and miR-137-3p) that were upregulated and two (miR-141-3p and miR-205-5p) that were downregulated in exosomes secreted by invasive BCa cells versus those secreted by noninvasive BCa cells [ 84 ]. However, the suitability of these miRNAs as liquid biomarkers to discriminate between NMIBC and MIBC requires further analysis.…”