MRN‐100, An Iron‐Based Compound, Possesses Anti‐HIV Activity In Vitro

Abstract

We examined the in vitro anti-human immunodeficiency virus (HIV) activity of MRN-100, an iron-based compound derived from bivalent and tervalent ferrates. MRN-100 action against HIV-1 (SF strain) was tested in primary cultures of peripheral blood mononuclear cells (MNC) by analyzing p24 antigen production and percent survival of MNC infected with HIV. MRN-100 at a concentration of 10% (v/v) inhibited HIV-1 replication in 11 out of 14 samples (79%). The percentage of suppression of p24 antigen was −12.3 to 100% at 10 days post-treatment. MRN-100 also exhibited a significant protective effect in the survival of HIV-1-infected MNC. MNC survival post-treatment was dose dependent, 70.4% ± 8.4, 83.6% ± 10.7 and 90% ± 11.4, at concentrations 2.5, 5 and 10% (v/v), respectively, as compared with 53% ± 4 for HIV-1-infected MNC without treatment. The effect was detected as early as 4 days and continued up to 11 days. Treatment with MRN-100 caused no significant change in proliferative response of MNC alone or cocultured with different mitogens: PHA and Con-A (activators of T cell function) and PWM (activator of CD4+ T cell-dependent B cells). We concluded that MRN-100 possesses anti-HIV activity in vitro and without an increase in lymphocyte proliferation, MRN-100 may be a useful agent for treating patients with acquired immunodeficiency syndrome.

An iron-based beverage, HydroFerrate fluid (MRN-100), alleviates oxidative stress in murine lymphocytes in vitro

“…Iron is incorporated into heme complexes which are typically associated with oxygen carrier proteins. In addition to its ability to protect against oxidative stress, we have shown elsewhere that MRN-100 also possesses anti-HIV activity [ 13 ].…”

“…MRN-100 is an iron-based compound derived from bivalent and trivalent ferrates and was prepared in distilled water (DW) with the concentration of Fe 2+ and Fe 3+ ions at ~2 × 10 -12 mol/l. MRN-100 is obtained from phytosin as previously described [ 13 ]. MRN-100 was provided by ACM Co., Ltd., Japan.…”

Protective effect of hydroferrate fluid, MRN-100, against lethality and hematopoietic tissue damage in γ-radiated Nile tilapia, Oreochromis niloticus

“…5 filter paper and the filtrate is evaporated and condensed in a water bath. The iron compound obtained is subjected to fractional determination with respect to bivalent ferrate and trivalent ferrate in order to generate MRN-100 [13, 21]. Briefly, the sample's Fe (II) quantity is determined using the o-phenanthrolin method.…”

Hydroferrate Fluid, MRN-100, Provides Protection against Chemical-Induced Gastric and Esophageal Cancer in Wistar Rats

“…Hydroferrate fluid, MRN-100, is an iron-based compound composed of bivalent and trivalent ferrates isolated from phytosin. Previous research on MRN-100 has shown its potential as a protector against age-associated oxidative stress 7 , γ-radiation 8 , and HIV activity 9 . The current study was a preliminary investigation of whether MRN-100 has the ability to restrict esophageal/gastric cancer in rats.…”