mRNA expression and metabolic regulation of npy and agrp1/2 in the zebrafish brain

Jeong, Inyoung; Kim, Eun‐Mi; Kim, Suhyun; Kim, Hwan Ki; Lee, Jun Young; Seong, Jae Young; Park, Hae Chul

doi:10.1016/j.neulet.2018.01.017

Cited by 38 publications

(23 citation statements)

References 28 publications

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“…This contrasts previous findings for this species (Murashita et al, 2009a), providing novel insights that may revise the current knowledge on orexigenic and anorexigenic factors in salmon. Our data are in agreement with the suggested AgRP orexigenic role for other vertebrates including several fish species, such as goldfish (Cerda-Reverter and Peter, 2003), zebrafish (Song et al, 2003;Jeong et al, 2018;Shainer et al, 2019), seabass (Dicentrarchus labrax) (Agulleiro et al, 2014), Ya fish (Schizothorax prenanti) (Wei et al, 2013), arctic char (Salvelinus alpinus) (Striberny et al, 2015), and coho salmon (Kim et al, 2015). The discrepancies observed between the results obtained in the present study and the previous studies from Valen et al (2011), which indicated that agrp1 have an anorexigenic role, are most likely a result from hypothalamus versus whole brain analysis.…”

Section: Discussionsupporting

confidence: 92%

Hypothalamic agrp and pomc mRNA Responses to Gastrointestinal Fullness and Fasting in Atlantic Salmon (Salmo salar, L.)

et al. 2020

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The orexigenic agouti-related protein (AgRP) and the anorexigenic pro-opiomelanocortin (POMC) are crucial players in the control of feed intake in vertebrates, yet their role in teleosts has not been fully established. Triplicate groups of Atlantic salmon (Salmo salar) post smolts were subjected to (1) fasting for 3 days (fast) and (2) normal feeding (fed), resulting in a significant (p < 0.05) upregulation of hypothalamic agrp1 transcripts levels in the fast group. Moreover, the mRNA abundance of agrp1 was significantly (p < 0.05) correlated with the stomach dry weight content. Corresponding inverse patterns were observed for pomca2, albeit not statistically significant. No significant differences were found for the other paralogues, agrp2 and pomca1 and b, between fed and fast groups. The significant correlation between stomach fullness and agrp1 mRNA expression suggests a possible link between the stomach filling/distension and satiety signals. Our study indicates that hypothalamic agrp1 acts as an orexigenic signal in Atlantic salmon.

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Section: Discussionsupporting

confidence: 92%

Hypothalamic agrp and pomc mRNA Responses to Gastrointestinal Fullness and Fasting in Atlantic Salmon (Salmo salar, L.)

et al. 2020

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“…One hypothesis is that reduced anatomical constraints from eye-loss allow for hypothalamic expansion. A number of hypothalamic neuropeptides are known to be upregulated in cavefish including HCRT and NPY, which localize to the lateral hypothalamus and periventricular/lateral hypothalamus respectively (Alié et al, 2018; Jaggard et al, 2018; Jeong et al, 2018). Both of these nuclei are larger across all three populations of cavefish.…”

Section: Discussionmentioning

confidence: 99%

An Adult Brain Atlas Reveals Broad Neuroanatomical Changes in Independently Evolved Populations of Mexican Cavefish

et al. 2019

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A shift in environmental conditions impacts the evolution of complex developmental and behavioral traits. The Mexican cavefish, Astyanax mexicanus, is a powerful model for examining the evolution of development, physiology, and behavior because multiple cavefish populations can be compared to an extant, ancestral-like surface population of the same species. Many behaviors have diverged in cave populations of A. mexicanus, and previous studies have shown that cavefish have a loss of sleep, reduced stress, an absence of social behaviors, and hyperphagia. Despite these findings, surprisingly little is known about the changes in neuroanatomy that underlie these behavioral phenotypes. Here, we use serial sectioning to generate brain atlases of surface fish and three independent cavefish populations. Volumetric reconstruction of serial-sectioned brains confirms convergent evolution on reduced optic tectum volume in all cavefish populations tested. In addition, we quantified volumes of specific neuroanatomical loci within several brain regions that have previously been implicated in behavioral regulation, including the hypothalamus, thalamus, and habenula. These analyses reveal an enlargement of the hypothalamus in all cavefish populations relative to surface fish, as well as subnuclei-specific differences within the thalamus and prethalamus. Taken together, these analyses support the notion that changes in environmental conditions are accompanied by neuroanatomical changes in brain structures associated with behavior. This atlas provides a resource for comparative neuroanatomy of additional brain regions and the opportunity to associate brain anatomy with evolved changes in behavior.

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“…Therefore, these antagonistic melanocortinergic populations, the orexigenic AGRP neurons and the anorexigenic POMC-expressing neurons, integrate metabolic, and nutrient information to regulate food intake. Recent experiments in fish suggest that the phenotype of AGRP neurons is a derived characteristic of mammalian brain since fish AGRP neurons does not produce NPY, however both zebrafish AGRP and NPY neurons are GABAergic and respond to fasting by increasing neuropeptide gene expression (46).…”

Section: Discussionmentioning

confidence: 99%

Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study

et al. 2019

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In mammals, glucosensing markers reside in brain areas known to play an important role in the control of food intake. The best characterized glucosensing mechanism is that dependent on glucokinase (GK) whose activation by increased levels of glucose leads in specific hypothalamic neurons to decreased or increased activity, ultimately leading to decreased food intake. In fish, evidence obtained in recent years suggested the presence of GK-like immunoreactive cells in different brain areas related to food intake control. However, it has not been established yet whether or not those neuronal populations having glucosensing capacity are the same that express the neuropeptides involved in the metabolic control of food intake. Therefore, we assessed through dual fluorescent in situ hybridization the possible expression of GK in the melanocortinergic neurons expressing proopiomelanocortin (POMC) or agouti-related protein (AGRP). POMC and AGRP expression localized exclusively in the rostral hypothalamus, in the ventral pole of the lateral tuberal nucleus, the homolog of the mammalian arcuate nucleus. Hypothalamic GK expression confined to the ependymal cells coating the ventral pole of the third ventricle but some expression level occurred in the AGRP neurons. GK expression seems to be absent in the hypothalamic POMC neurons. These results suggest that AGRP neurons might sense glucose directly through a mechanism involving GK. In contrast, POMC neurons would not directly respond to glucose through GK and would require presynaptic inputs to sense glucose. Ependymal cells could play a critical role relying glucose metabolic information to the central circuitry regulating food intake in fish, especially in POMC neurons.

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mRNA expression and metabolic regulation of npy and agrp1/2 in the zebrafish brain

Cited by 38 publications

References 28 publications

Hypothalamic agrp and pomc mRNA Responses to Gastrointestinal Fullness and Fasting in Atlantic Salmon (Salmo salar, L.)

Hypothalamic agrp and pomc mRNA Responses to Gastrointestinal Fullness and Fasting in Atlantic Salmon (Salmo salar, L.)

An Adult Brain Atlas Reveals Broad Neuroanatomical Changes in Independently Evolved Populations of Mexican Cavefish

Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study

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