mRNA Poly (A) Tail, a 3′ Enhancer of Translational Initiation

Munroe, David J.; Jacobson, Allan

doi:10.1128/mcb.10.7.3441-3455.1990

Cited by 59 publications

(39 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The poly(A) tract has been shown to play a role both in translation and RNA stability in many systems (for reviews see [10][11][12]. The longer the poly(A) tract of eukaryotic cytosolic RNAs, the greater the translational efficiency of the RNA (13)(14)(15)(16). In the eukaryotic cytoplasm, polyadenylation also *To whom correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 99%

Coordination of kRNA editing and polyadenylation in Trypanosoma brucei mitochondria: complete editing is not required for long poly(A) tract addition

Militello

Read

1999

Nucleic Acids Research

View full text Add to dashboard Cite

Mitochondrial RNAs in Trypanosoma brucei are post-transcriptionally modified by the addition and deletion of uridylate residues in a process called kRNA editing. Unedited, partially edited and fully edited RNAs exist in the steady-state RNA population. Previous experiments have demonstrated that T.brucei mitochondrial RNAs contain both short (approximately 20 nt) and long (120-200 nt) poly(A) tracts. However, it is unknown exactly what poly(A) tract lengths are present on unedited, partially edited and fully edited RNAs. To gain insight into the role of the poly(A) tract in T.brucei mitochondria, ribosomal protein S12 (RPS12) RNAs with short and long poly(A) tracts were purified by hybrid selection and analyzed by RT-PCR and DNA sequencing. Unedited RPS12 RNAs were found almost exclusively in populations with short poly(A) tracts. Both partially and fully edited RPS12 RNAs were found in populations with short and long poly(A) tracts. Therefore, there is a correlation between the presence of editing and the presence of the long poly(A) tract. Since a proportion of partially edited RPS12 RNAs contain long poly(A) tracts, it is unlikely that the long poly(A) tract is the sole signal for translation. Other implications for the role of polyadenylation in mitochondrial gene regulation are discussed.

show abstract

Section: Introductionmentioning

confidence: 99%

Coordination of kRNA editing and polyadenylation in Trypanosoma brucei mitochondria: complete editing is not required for long poly(A) tract addition

Militello

Read

1999

Nucleic Acids Research

View full text Add to dashboard Cite

show abstract

“…5). These proteins are enriched for biological functions such as translation initiation (eIF5A, eIF4B), ribosome biogenesis (RIOK1), and poly(A) RNA binding, which is also related to translation 58, 59 . The CK2 site in RIOK1 (Ser21/22) was critical for RIOK to support proliferation/survival of A549 cells.…”

Section: Discussionmentioning

confidence: 99%

CK2 signaling from TOLLIP-dependent perinuclear endosomes is an essential feature ofKRASandNRASmutant cancers

Basu

Luke

Karim

et al. 2022

Preprint

View full text Add to dashboard Cite

Oncogenic RAS induces perinuclear translocation of the oncogenic kinases ERK1/2 and CK2 and the MAPK scaffold KSR1, forming endosomal signaling hubs termed perinuclear signaling centers (PSCs). PSCs are found in nearly all cancer cell lines and tumor tissues, suggesting that compartmentalization of oncogenic kinases drives tumorigenesis. We show here that the endosomal adaptor, TOLLIP, tethers RAB11A+ signaling endosomes containing CK2 and KSR1, but not ERK, to the perinuclear ER. TOLLIP binds to the KSR1 CA5 pseudo-kinase domain through a conserved region predicted to form a β-hairpin, thus anchoring PSCs to perinuclear endosomes. TOLLIP is perinuclear in cancer cells and KRasG12D-driven mouse tumors but is pan-cytoplasmic in non-transformed cells, correlating with the presence of PSCs. TOLLIP is required for proliferation/survival of KRAS mutant tumor cells but not HRAS, NRAS and BRAF cancers or non-transformed cells. KRasG12D-induced lung lesions in TollipKO/KO mice showed reduced numbers of carcinomatous lesions, implicating TOLLIP in progression to malignant cancer. Phosphoproteomics studies revealed that perinuclear CK2 phosphorylates specific substrates, particularly proteins involved in ribosome biogenesis and translation such as RIOK1 and eIF4B. In summary, our findings identify TOLLIP as a key signaling adaptor in KRAS tumors whose inhibition is a potential vulnerability of these cancers.

show abstract

“…This was achieved after acquiring the added tPA protein sequence from UniProtKB by using accession ID: P00750 [ 54 ]. Furthermore, to ensure stability of the mRNA-based vaccine design, the addition of elements specific to eukaryotic mRNAs [ 55 , 56 ] was performed. This included the addition of a 5′ cap, poly (A) tail, and 5′ and 3′ untranslated regions.…”

Section: Methodsmentioning

confidence: 99%

Potential Immunogenic Activity of Computationally Designed mRNA- and Peptide-Based Prophylactic Vaccines against MERS, SARS-CoV, and SARS-CoV-2: A Reverse Vaccinology Approach

Khan

Ansari

et al. 2022

Molecules

View full text Add to dashboard Cite

The continued emergence of human coronaviruses (hCoVs) in the last few decades has posed an alarming situation and requires advanced cross-protective strategies against these pandemic viruses. Among these, Middle East Respiratory Syndrome coronavirus (MERS-CoV), Severe Acute Respiratory Syndrome coronavirus (SARS-CoV), and Severe Acute Respiratory Syndrome coronavirus-2 (SARS-CoV-2) have been highly associated with lethality in humans. Despite the challenges posed by these viruses, it is imperative to develop effective antiviral therapeutics and vaccines for these human-infecting viruses. The proteomic similarity between the receptor-binding domains (RBDs) among the three viral species offers a potential target for advanced cross-protective vaccine designs. In this study, putative immunogenic epitopes including Cytotoxic T Lymphocytes (CTLs), Helper T Lymphocytes (HTLs), and Beta-cells (B-cells) were predicted for each RBD-containing region of the three highly pathogenic hCoVs. This was followed by the structural organization of peptide- and mRNA-based prophylactic vaccine designs. The validated 3D structures of these epitope-based vaccine designs were subjected to molecular docking with human TLR4. Furthermore, the CTL and HTL epitopes were processed for binding with respective human Lymphocytes Antigens (HLAs). In silico cloning designs were obtained for the prophylactic vaccine designs and may be useful in further experimental designs. Additionally, the epitope-based vaccine designs were evaluated for immunogenic activity through immune simulation. Further studies may clarify the safety and efficacy of these prophylactic vaccine designs through experimental testing against these human-pathogenic coronaviruses.

show abstract

mRNA Poly (A) Tail, a 3′ Enhancer of Translational Initiation

Cited by 59 publications

References 87 publications

Coordination of kRNA editing and polyadenylation in Trypanosoma brucei mitochondria: complete editing is not required for long poly(A) tract addition

Coordination of kRNA editing and polyadenylation in Trypanosoma brucei mitochondria: complete editing is not required for long poly(A) tract addition

CK2 signaling from TOLLIP-dependent perinuclear endosomes is an essential feature ofKRASandNRASmutant cancers

Potential Immunogenic Activity of Computationally Designed mRNA- and Peptide-Based Prophylactic Vaccines against MERS, SARS-CoV, and SARS-CoV-2: A Reverse Vaccinology Approach

Contact Info

Product

Resources

About