mRNA Turnover in Trypanosomes

Clayton, Christine

doi:10.1007/978-3-642-28687-2_4

Cited by 2 publications

(2 citation statements)

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“…Although trypanosomes are very successful in adapting to changing conditions in different hosts, the parasites have almost no transcriptional control of gene expression and heavily rely on the post‐transcriptional gene regulation [9]. Interaction between the cis regulatory elements in the 3′ untranslated region (UTR) of mRNAs and the trans acting RBPs are indispensable for this kind of regulation [10,11]. In higher eukaryotes, several ARE binding proteins (AUBPs), with multiple regulatory roles on the ARE containing mRNAs, have been discovered.…”

Section: Introductionmentioning

confidence: 99%

The DRBD13 RNA binding protein is involved in the insect‐stage differentiation process of Trypanosoma brucei

et al. 2015

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Edited by Michael IbbaKeywords: DRBD13 Post transcriptional gene regulatory program Insect stage differentiation process Trypanosoma brucei a b s t r a c t DRBD13 RNA-binding protein (RBP) regulates the abundance of AU-rich element (ARE)-containing transcripts in trypanosomes. Here we show that DRBD13 regulates RBP6, the developmentally critical protein in trypanosomatids. We also show DRBD13-specific regulation of transcripts encoding cell surface coat proteins including GPEET2, variable surface glycoprotein (VSG) and invariant surface glycoprotein (ISG). Accordingly, alteration in DRBD13 levels leads to changes in the target mRNA abundance and parasite morphology. The high consistency of the observed phenotype with known cell membrane exchanges that occur during progression of T. brucei through the insect stage of its life cycle suggests that DRBD13 is an important regulator in this largely unknown developmental process.

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Section: Introductionmentioning

confidence: 99%

The DRBD13 RNA binding protein is involved in the insect‐stage differentiation process of Trypanosoma brucei

et al. 2015

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“…Gene expression is therefore mainly controlled at the levels of mRNA turnover and translation [22]. In both trypanosomes [22], [23] and other eukaryotes [24], RNA binding proteins play a major role in this regulation.…”

Section: Introductionmentioning

confidence: 99%

The Trypanosome Pumilio Domain Protein PUF5

2013

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PUF proteins are a conserved family of RNA binding proteins found in all eukaryotes examined so far. This study focussed on PUF5, one of 11 PUF family members encoded in the Trypanosoma brucei genome. Native PUF5 is present at less than 50000 molecules per cell in both bloodstream and procyclic form trypanosomes. C-terminally myc-tagged PUF5 was mainly found in the cytoplasm and could be cross-linked to RNA. PUF5 knockdown by RNA interference had no effect on the growth of bloodstream forms. Procyclic forms lacking PUF5 grew normally, but expression of PUF5 bearing a 21 kDa tandem affinity purification tag inhibited growth. Knockdown of PUF5 did not have any effect on the ability of trypanosomes to differentiate from the mammalian to the insect form of the parasite.

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mRNA Turnover in Trypanosomes

Cited by 2 publications

References 93 publications

The DRBD13 RNA binding protein is involved in the insect‐stage differentiation process of Trypanosoma brucei

The DRBD13 RNA binding protein is involved in the insect‐stage differentiation process of Trypanosoma brucei

The Trypanosome Pumilio Domain Protein PUF5

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