The aim of this study was to determine the prevalence and characteristics of methicillin-resistant Staphylococcus aureus (MRSA) isolates from the patients, personnel and environments of a hospital in Sanandaj. During this study, 30 MRSA isolates were collected and analyzed with using spa typing method. Out of the 30 S. aureus isolates, 28 (93.33%) was methicillin resistant (MRSA), 16 spa types (t030, t711, t002, t1556, t325, t267, t1358, t230 , t2422, t969, t1814, t037, t300, t044, t180, and t5598) were found in different wards of the hospital. The most frequent were t030 (17.2 %), t711 (13.7 %). Our data demonstrated that the overall, resistances to erythromycin, gentamycin, and ciprofloxacin, were most frequent (87.5, 81.25, and 62.5 respectively).