MRSA Transmission between Cows and Humans

Juhász-Kaszanyitzky, Éva; Jánosi, Szilárd; Somogyi, P; Dán, Ádám; Bloois, Linda van der Graaf–van; Duijkeren, Engeline van; Wagenaar, Jaap A.

doi:10.3201/eid1304.060833

Cited by 283 publications

(259 citation statements)

References 14 publications

Supporting

Mentioning

244

Contrasting

Unclassified

Order By: Relevance

“…By SCCmec typing the strains were classified as community-associated MRSA (CA-MRSA) that are characterized by the presence of smaller cassette elements of SCCmec types IV and V (Boyle-Vavra and Daum, 2007). Detection of SCCmec type IV and V in animals was also reported in other studies (Juhasz-Kaszanyitzky et al, 2007;Khanna et al, 2008).…”

Section: Discussionsupporting

confidence: 85%

Occurrence of methicillin-resistant strains of Staphylococcus aureus at a goat breeding farm

Šťástková¹,

Karpíšková²,

Karpíšková³

2009

Vet. Med. - Czech

View full text Add to dashboard Cite

ABSTRACT:The aim of this study was to report the detection of methicillin-resistant Staphylococcus aureus (MRSA) strains at a veterinary university goat breeding farm and their characteristics. A total of 278 samples collected from animals, milk, environment and farm personnel between June 2006 and March 2008 were examined. The identification of S. aureus isolates was performed by a species specific PCR assay. All detected isolates were tested for resistance to oxacillin and other antimicrobials by phenotypic methods and for the mecA gene by PCR method. Eight MRSA were detected in this study. Five of them originated from goat's milk and three were recovered from one human carrier of the farm personnel. All obtained MRSA isolates were clonally consistent and were characterized as: SCCmec type IV, spa type t064, seb positive and for genes encoding TSST-1, PVL and exfoliative toxins A and B negative.

show abstract

Section: Discussionsupporting

confidence: 85%

Occurrence of methicillin-resistant strains of Staphylococcus aureus at a goat breeding farm

Šťástková¹,

Karpíšková²,

Karpíšková³

2009

Vet. Med. - Czech

View full text Add to dashboard Cite

show abstract

“…All cefoxitin resistant S. aureus were also resistant to penicillin G. Out of the (77.2%) cefoxitin resistant S. aureus isolates, (95.6%) of were also resistant to Penicillin G. This is an indicator of MRSA [38][39][40]. This is due to the fact that resistance of S. aureus to these drugs may be attributed to the production of β-lactamase, an enzyme that inactivates penicillin and closely related antimicrobials [41,42].…”

Section: Discussionmentioning

confidence: 99%

Isolation, Identification and Antimicrobial Resistance Profile of Staphylococcus aureus and Occurrence of Methicillin Resistant S. aureus Isolated from Mastitic Lactating Cows in and around Assosa Town, Benishangul Gumuz Region, Ethiopia

Tassew¹

2017

JDVAR

View full text Add to dashboard Cite

A cross -sectional study was conducted from November 2016 to May 2017 in Dairy cattle in and around Asossa town in order to estimate the prevalence of mastitis, to identify S. aurues from mastitic lactating cows, to evaluate its antimicrobial resistance pattern and to identify risk factors associated with mastitis. A total of 384 Dairy cows milk samples were collected using purposive sampling techniques. The overall prevalence of mastitis at cow level was 39.32% with 11.45% and 27.86% of clinical and subclinical mastitis, respectively. In this study, the subclinical mastitis was significantly higher than clinical mastitis. For all except Age and parity, the multivariable logistic regression analysis for intrinsic and extrinsic risk factors showed significant difference in the prevalence of mastitis in the study area (P˂0.05). From 151 mastitis infected lactating cows, 436 milk samples were cultured and 22.14% S. aureus were isolated. Presumptively identified S. aureus isolates were subjected to antimicrobial susceptibility test and 63 (74.11%) MRSA have been identified from a total of 85 S. aureus using cefoxitin through disk diffusion method. The present result showed a significant association of resistance pattern with S. aureus isolates, particularly to penicillin G (95.55%), Cefoxitin (77.19%), Tetracycline (63.41%), Streptomycin (60.78%), Gentamycin (59.37%), Vancomycin (56.75%), Clindamycine (54.35%) and Bacitracin (53.65%). In this study, 77.19% S. aureus isolates were found resistant against Cefoxitin. There were also observed multidrug resistance, mainly to Penicillin G, Streptomycin and Tetracycline. The present study revealed higher prevalence of mastitis and occurrence of multidrug resistance S. aureus specifically which belongs to the MRSA which are dependent on multiple associated risk factors. Hence, regular resistance follow-up, using antimicrobials sensitivity tests helps to select effective antimicrobials and to reduce the problems of drug resistance developments towards commonly used antimicrobials.

show abstract

“…Particularly, methicillin-resistant S. aureus (MRSA), which are resistant to almost all types of β-lactam antimicrobials, have been a concern worldwide (Lee 2003, Van Duijkeren et al 2004. The presence of MRSA in dairy cows has been investigated because it was demonstrated that cattle may serve as a source of emergence of new MRSA strains in humans (Juhász-Kaszanyitzky et al 2007, Vanderhaeghen et al 2010. Therefore, colonization of dairy cattle with antimicrobial-resistant S. aureus impacts milk production and may additionally represent an infection hazard to people who work in close contact with cows or consume raw milk (Juhász-Kaszanyitzky et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil

et al. 2016

View full text Add to dashboard Cite

In intensive dairy farming, persistent intramammary infection has been associated with specific Staphylococcus (S.) aureus strains, and these strains may be resistant to antimicrobials. The objective of this study was to evaluate the antimicrobial resistance phenotypes of S. aureus isolates and to assess the distribution and the persistence of clonal groups in small dairy herds of southern Brazil. Milk samples were collected from all lactating cows from 21 dairy farms over a two-year period, totaling 1,060 samples. S. aureus isolates were tested for susceptibility to thirteen antimicrobials using the disk diffusion method. The total DNA of the isolates was subjected to SmaI digestion followed by pulsed-field gel electrophoresis (PFGE). Banding patterns differing by ≤4 bands were considered members of a single PFGE cluster. The frequency of S. aureus isolation ranged from 3.45% to 70.59% among the 17 S. aureus-positive herds. Most S. aureus isolates (87.1%) were susceptible to all antimicrobials; resistance to penicillin (18.2%) was the most frequently observed. The 122 isolates subjected to macrorestriction analysis were classified into 30 PFGE-clusters. Among them, only 10 clusters were intermittent or persistent over the two-year period. The majority (93.6%) of isolates belonging to persistent and intermittent clusters were susceptible to all tested antimicrobials. S. aureus intramammary colonization in small dairy farms of southern Brazil is most frequently caused by sporadic PFGE clusters, although some persistent clusters can arise over time. Both sporadic and persistent isolates were highly susceptible to antimicrobials.

show abstract

MRSA Transmission between Cows and Humans

Cited by 283 publications

References 14 publications

Occurrence of methicillin-resistant strains of Staphylococcus aureus at a goat breeding farm

Occurrence of methicillin-resistant strains of Staphylococcus aureus at a goat breeding farm

Isolation, Identification and Antimicrobial Resistance Profile of Staphylococcus aureus and Occurrence of Methicillin Resistant S. aureus Isolated from Mastitic Lactating Cows in and around Assosa Town, Benishangul Gumuz Region, Ethiopia

Antimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil

Contact Info

Product

Resources

About