MS/MS and UPLC-MS profiling of triterpenoid saponins from leaves and roots of four red beet (Beta vulgaris L.) cultivars

Mroczek, Agnieszka; Kapusta, Ireneusz; Stochmal, Anna; Janiszowska, Wirginia

doi:10.1016/j.phytol.2019.02.015

Cited by 16 publications

(26 citation statements)

References 18 publications

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“…Saponins were quantitated using ultra-performance reverse-phase liquid chromatography (UPLC) coupled with ESI-MS/MS described by Mroczek et al [52]. Analyses were carried out using an Acquity ultra-performance liquid chromatograph (Waters) coupled with an Acquity TQD tandem quadrupole mass spectrometer with an ESI source.…”

Section: Methodsmentioning

confidence: 99%

Assessment of the Bioactive Compounds in White and Red Wines Enriched with a Primula veris L.

et al. 2019

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The aim of this paper was to analyze selected physicochemical properties and the pro-health potential of wines produced in southeastern Poland, in the Subcarpathian region, and commercial Carlo Rossi wines enhanced with cowslip (Primula veris L.). This study used ultra-performance reverse-phase liquid chromatography (UPLC)-PDA-MS/MS to perform most of the analysis, including the polyphenolic compounds and saponin content in wines enriched by Primula veris L. The initial anthocyanin content in Subcarpathian (Regional) red wine samples increased four times to the level of 1956.85 mg/L after a 10% addition of Primula veris L. flowers. For white wines, a five-fold increase in flavonol content was found in Subcarpathian (Regional) and wine samples, and an almost 25-fold increase in flavonol content was found in Carlo Rossi (Commercial) wine samples at the lowest (2.5%) Primula veris L. flower addition. Qualitative analysis of the regional white wines with a 10% Primula veris L. flower enhancement demonstrated the highest kaempferol content (197.75 mg/L) and a high quercetin content (31.35 mg/L). Thanks to wine enrichment in triterpenoid saponins and in polyphenolic compounds from Primula veris L. flowers, which are effectively extracted to wine under mild conditions, both white and red wines can constitute a highly pro-health component of diets, which is valuable in preventing chronic heart failure.

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Section: Methodsmentioning

confidence: 99%

Assessment of the Bioactive Compounds in White and Red Wines Enriched with a Primula veris L.

et al. 2019

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“…Various cultivated crops are supplemented with multiple saponins, which are responsible for plant health [34,55]. Likewise, triterpenoid saponins are the main part of the defense system of several agricultural crops, e.g., Spinacia oleracea, Beta vulgaris, Glycyrrhiza glabra, Helianthus annuus, Aesculus hippocastanum, Quillaja saponaria, Smilax ornata, and Camellia sinensis [20,34,[56][57][58]. On the other hand, various steroidal glycosides are also a prominent part of many plants, which are helpful in the plant immune system, e.g., Allium spp., Asparagus officinalis, Avena sativa, Capsicum spp., Dioscorea spp., Panax ginseng, Solanum melongena, S. lycopersicum, as well as Trigonella foenumgraecum [59][60][61][62][63].…”

Section: Types Of Plant Saponinsmentioning

confidence: 99%

Saponins in Insect Pest Control

Qasim¹,

Islam²,

Ashraf³

et al. 2019

Reference Series in Phytochemistry

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“…The profi le of triterpenoid saponins in the roots of red beet cv. Red Sphere (Beta vulgaris L.) was previously studied using reversed liquid chromatography and mass spectrometry by Mroczek et al [2012Mroczek et al [ , 2019 and 13 saponins have been described wherein 11 compounds contained oleanolic acids aglycone. Another research on saponins in red beet root cv.…”

Section: Introductionmentioning

confidence: 99%

“…The structural characterisation of triterpenoid saponins in the roots of red beet is very diffi cult and time-consuming due to the complexity of the matrix [Mikołajczyk-Bator et al, 2016;Mroczek et al, 2012Mroczek et al, , 2019, therefore, fractionation of the crude samples enables faster and more accurate identifi cation of signifi cantly preconcentrated saponins [Thakur et al, 2014]. High-speed counter-current chromatography is a very important technique in the separation of natural plant compounds [Jerz et al, 2008[Jerz et al, , 2010Spórna-Kucab et al, 2019;Wybraniec et al, 2009Wybraniec et al, , 2010 which enables subsequent identifi cation of new structures of target compounds [Choi et al, 2015;Figueiredo et al, 2017].…”

Section: Introductionmentioning

confidence: 99%

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High-Speed Counter-Current Chromatography in Separation and Identification of Saponins from Beta vulgaris L. Cultivar Red Sphere

Spórna‐Kucab¹,

Wybraniec²

2020

Pol. J. Food Nutr. Sci.

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Saponins, natural plant compounds exhibiting health benefi ts, were extracted from B. vulgaris L. cultivar (cv.) Red Sphere and separated by high-speed counter-current chromatography (HSCCC) in a new solvent system composed of: TBME-BuOH-ACN-H 2 O (1:2:1:5, v/v/v/v). The system was used in the head-to-tail mode. The fl ow rate of the mobile phase was 3 mL/min and the column rotation speed was 860 rpm. The retention of the stationary phase was 47%. The LC-MS/MS analyses were performed for the identifi cation of separated saponins in the crude extract as well as HSCCC fractions,. Signifi cantly different elution orders of the analytes were observed in the HSCCC and HPLC systems, indicating the complementarity of both the techniques in the fractionation of saponins. Moreover, during the HSCCC separation, acetal-type saponins were eluted faster than pentose/ hexose-type saponins and then dioxolane-type saponins. The separation of saponins in the HSCCC solvent system enabled the fractionation and preconcentration of 13 saponins for further fragmentation experiments in the LC-MS/MS system. Nine saponins were detected for the fi rst time ever in B. vulgaris L. cv. Red Sphere.

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MS/MS and UPLC-MS profiling of triterpenoid saponins from leaves and roots of four red beet (Beta vulgaris L.) cultivars

Cited by 16 publications

References 18 publications

Assessment of the Bioactive Compounds in White and Red Wines Enriched with a Primula veris L.

Assessment of the Bioactive Compounds in White and Red Wines Enriched with a Primula veris L.

Saponins in Insect Pest Control

High-Speed Counter-Current Chromatography in Separation and Identification of Saponins from Beta vulgaris L. Cultivar Red Sphere

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