MS2 RNA aptamer enhances prime editing in rice

Chai, Yiping; Jiang, Yuanyuan; Wang, Junya; Qiao, Dexin; Zhang, Yu; Xin, Cuiping; Zhou, Yun; Wang, Xuechen; Chen, Qijun

doi:10.1101/2021.10.20.465209

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…Extension of this strategy in plants significantly enhanced prime editing efficiency (Jiang et al, 2022b; Li et al, 2022b; Zou et al, 2022) (Table 2). In addition, MS2‐based PE (MS2‐PE) has also been developed to improve the prime editing efficiency by using RNA aptamers (MS2 and f6) in pegRNA and fusion of their binding protein MCP with the PE2 system (Figure 3F), and achieved up to 10.1‐fold increase in editing efficiency at five of six targets in transgenic rice lines (Chai et al, 2021).…”

Section: Prime Editors and Their Applications In Plantsmentioning

confidence: 99%

Plant base editing and prime editing: The current status and future perspectives

Zhang

et al. 2023

JIPB

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Precise replacement of an allele with an elite allele controlling an important agronomic trait in a predefined manner by gene editing technologies is highly desirable in crop improvement. Base editing and prime editing are two newly developed precision gene editing systems which can introduce the substitution of a single base and install the desired short indels to the target loci in the absence of double-strand breaks and donor repair templates, respectively. Since their discoveries, various strategies have been attempted to optimize both base editor (BE) and prime editor (PE) in order to improve the precise editing efficacy, specificity, and expand the targeting scopes. Here, we summarize the latest development of various BEs and PEs, as well as their applications in plants. Based on these progresses, we recommend the appropriate BEs and PEs for both basic plant research and crop improvement. Moreover, we propose the perspectives for further optimization of these two editors. We envision that both BEs and PEs will become the routine and customized precise gene editing tools for both plant biological research and crop improvement in the near future.

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Section: Prime Editors and Their Applications In Plantsmentioning

confidence: 99%

Plant base editing and prime editing: The current status and future perspectives

Zhang

et al. 2023

JIPB

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Optimized prime editing in monocot plants using PlantPegDesigner and engineered plant prime editors (ePPEs)

Jin

Lin

Gao³

et al. 2022

Nat Protoc

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Prime-editing methods and pegRNA design programs

Mikhaylova,

Kuluev,

Gerashchenkov

et al. 2024

Molekulârnaâ biologiâ

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It has been 10 years since CRISPR/Cas technology was applied to edit the genomes of various organisms. Its ability to produce a double-strand break in a DNA region specified by the researcher started a revolution in bioengineering. Later, the Base Editing (BE) method was developed. BE is performed via the formation of single-strand breaks by the mutant form of Cas nuclease (nickase), cross-linked with deaminases and other enzymes. It can be used to promote A↔G and C↔T transitions, and a C→G transversion. Just over three years ago, a new Prime Editing (PE) variant of CRISPR/Cas was invented. Unlike BE, in PE the nickase is fused with reverse transcriptase, capable of building a new DNA chain using the pegRNA template. The pegRNA consists of an elongated guide RNA with an extra sequence at the 3ꞌ-end. Prime editing makes it possible to insert the desired mutations into this extra sequence and to carry out any substitutions and indels of bases without the use of special donor DNA. To date, a number of PE variants have been proposed, and they are briefly considered in this review with an emphasis on prime editing of plant genomes. Some attention is also paid to pegRNA design programs, as well as evaluation of the efficiency of the editing. Such a variety of PE techniques is due to the opportunities of high-precision introduction of desired changes with a rather low frequency of off-target mutations in the genomes of various organisms. The relatively low efficiency of prime editing inspire researchers to offer new approaches. There is hope that further development of the technology will improve PE enough to take its rightful place among the genome targeting methods, suitable for any organisms, and will have a positive impact on the agricultural sector, industrial biotechnologies, and medicine.

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MS2 RNA aptamer enhances prime editing in rice

Cited by 3 publications

References 21 publications

Plant base editing and prime editing: The current status and future perspectives

Plant base editing and prime editing: The current status and future perspectives

Optimized prime editing in monocot plants using PlantPegDesigner and engineered plant prime editors (ePPEs)

Prime-editing methods and pegRNA design programs

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