MT1-MMP expression in the odontogenic region of rat incisors undergoing interrupted eruption

Omar, Nádia Fayez; Gomes, José Rosa; Neves, Juliana dos Santos; Salmon, Cristiane R.; Novaes, Pedro Duarte

doi:10.1007/s10735-011-9356-0

Cited by 16 publications

(17 citation statements)

References 35 publications

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“…Other studies using procedures such as resection, transection, immobilization, and use of drugs to evaluate the eruption of the rat incisor have not demonstrated a role for preameloblast and preodontoblast cell proliferation on the eruption rate (Berkovitz and Thomas, ; Berkovitz, ; Melcher and Correia, ; Moxham and Berkovitz, ; Tsuruta et al, ; Pitaru et al, ; Chiba et al, ; Michaeli et al, ; Moxham and Berkovitz, ; Taverne et al, ; Teng et al, ; Burn‐Murdoch, ; Neves et al, ; Omar et al, ). Likewise, these studies also did not demonstrate a role for the dental follicle in the incisor eruption process in order to confirm the dental follicle theory proposed for molar teeth.…”

Section: Discussionmentioning

confidence: 99%

“…It is easy to change the experimental conditions of rat incisor eruption using, for examples, the resection of the root or odontogenic region (Berkovitz and Thomas, ; Moxham and Berkovitz, ), transections at different levels (Berkovitz and Thomas, ; Berkovitz, ; Pitaru et al, ; Merzel et al, ; Merzel et al, ), mechanical interruption (Melcher and Correia, ; Michaeli et al, ; Omar et al, ) and injection of drugs that act on collagen in the periodontal ligament or on cell proliferation (Tsuruta et al, ; Michaeli et al, ; Chiba et al, ; Moxham and Berkovitz, ; Taverne et al, ; Teng et al, ; Burn‐Murdoch, ; Gerlach et al, ).…”

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confidence: 99%

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Relationship Between Cell Proliferation and Eruption Rate in the Rat Incisor

Gomes

Omar

Carmo

et al. 2013

The Anatomical Record

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The aim of this study was to further define the relationship between cell proliferation and the rate of tooth eruption in the rat incisor. Vinblastine is a drug that blocks cellular mitosis and was used to inhibit cell proliferation in the odontogenic region of rat incisors that were submitted to a shortening treatment or to higher masticatory forces. Male Wistar rats were divided into five groups: normofunctional (control group for incisor eruption), hypofunctional (incisor submitted to eruption acceleration), hyperfunctional (incisors under higher masticatory forces), hypofunctional with vinblastine and hyperfunctional with vinblastine. In incisors submitted to shortening procedures, a significant decrease in the eruption rate and cell proliferation was observed two days after vinblastine injection, suggesting that incisor eruption is dependent on cell proliferation. Anat

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Relationship Between Cell Proliferation and Eruption Rate in the Rat Incisor

Gomes

Omar

Carmo

et al. 2013

The Anatomical Record

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“…MMPs play an important role in collagen matrix remodelling in physiologic and pathologic processes, such as those found in basal membranes, dental follicle tissue and tumour metastasis [27,29]. Although MMP-2 is related to ameloblastoma pathogenesis, it seems to be constitutively expressed in physiologic tissues and many cell types and to exhibit characteristics of a housekeeping gene [30-32].…”

Section: Discussionmentioning

confidence: 99%

Epigenetic regulation of matrix metalloproteinase expression in ameloblastoma

et al. 2012

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BackgroundAn ameloblastoma is a benign odontogenic neoplasm with aggressive behaviour and high recurrence rates. The increased expression of matrix metalloproteinases (MMPs) has been reported in ameloblastomas. In the present study, we hypothesised that epigenetic alterations may regulate MMP expression in ameloblastomas.MethodsWe investigated the methylation status of the genes MMP-2 and MMP-9 in addition to mRNA transcription and protein expression in ameloblastomas. Methylation analysis was performed by both methylation-specific polymerase chain reaction (MSP-PCR) and restriction enzyme digestion to evaluate the methylation profile of MMP-2 and MMP-9 in 12 ameloblastoma samples and 12 healthy gingiva fragments, which were included as controls. Furthermore, we investigated the transcription levels of the genes by quantitative reverse-transcription PCR (qRT-PCR). Zymography was performed to verify protein expression in ameloblastomas.ResultsThe ameloblastomas showed a high frequency of unmethylated MMP-2 and MMP-9, whereas the healthy gingival samples presented a sharp prevalence of methylated MMPs. Higher expression levels of MMP-9 were found in ameloblastomas compared to healthy gingiva. However, no significant differences in the MMP-2 mRNA expression between groups was found. All ameloblastomas showed positive expression of MMP-2 and MMP-9 proteins.ConclusionsOur findings suggest that expression of MMP-9 is increased in ameloblastomas and is possibly modulated by unmethylation of the gene.

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“…However, in this decade it has been demonstrated that another kind of enzymes denominated matrix metalloproteinases seem to have important roles during the development, growth and morphological maturation of different parts of the body like bones (Holmbeck et al, 1999; Yang et al, 2008), vascular system, skeletal muscle (Oh et al, 2004s), kidney and dental structures (Gomes et al, 2010, 2011, Omar et al, 2011, 2017).…”

Section: Introductionmentioning

confidence: 99%

Are the Matrix Metalloproteinases 2 and 9 beeing expressed in the mucosa of the rat small intestine during its intrauterine and postnatal life development ?

Reis

Gomes

2019

Preprint

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MMP-2 and MMP-9 are proteins with well stablished roles on the remodeling of tissue during morphogenesis. This study aimed to evaluate the activity and expression of the MMP-2 and MMP-9 in the rat small intestine mucosa layer on 15 th and 18 th days of intratuterine life (i.u.) and at 3rd, 10 th , 17 th, 25 th , and 32 th days after birth (a.b.). Samples were submitted to zimography, immunohistochemistry methods and Masson trichrome staining. Results showed that MMP-2 and MMP-9 were not expressed in the small intestine epithelium during intrauterine life.MMP-9 was immunolocalized in the villi goblet cells and in the lamina propria in cells identified as being the mast cells (a.b.). We concluded that in the i.u. and a.b. life the MMP-2 and MMP-9 were not expressed in the small intestine epithelium. However, after birth, because MMP-9 was expressed in the mast cels present in the lamina propria it may be involved in the remodeling process of the innate immunity that occurs during the small intestine development.key words: rat small intestine, MMP-2, MMP-9, development, mast cells During the suckling and weaning phases, the intestinal epithelium becomes completely developed, and two compartments can be identified; the villi (functional compartment) and the crypts, which are considered the proliferative compartment where cells are produced and differentiated, before their migration to the villi to execute their digestive functions.The suckling phase represents the period from birth until the 16th day of life, where milk is the main food. Around the 17th day, during the third week of life, diet with milk is changed to diet with solid food (weaning phase). Therefore, during the first three weeks after birth, the mucosa layer , specially the epithelium, goes through several morphological and physiological remodeling related to digestive and absorptive processes. These functional changes are produced by a shift in the expression of different enzymes present in the cell membrane like lactase, maltase, sucrase and intestinal alkaline phosphatase (Goldstein et al, 1971; Uezato and Fujita, 1983;Tojyo, 1984; Gomes et al., 2016).However, in this decade it has been demonstrated that another kind of enzymes denominated matrix metalloproteinases seem to have important roles during the development, growth and morphological maturation of different parts of the body like bones (Holmbeck et al, 1999; Yang et al., 2008), vascular system, skeletal muscle (Oh et al., 2004s), kidney and dental structures (Gomes et al., 2010, 2011, Omar et al., 2011, 2017.Metalloproteinases found in the extracellular matrix are denominated matrix metalloproteinases (MMPs) such as MMP-2 and MMP-9 (also denominated gelatinases) or may be anchored in the cell membrane which is denominated membrane matrix metalloproteinase (MT-MMPs). Despite this, there are still few descriptions about the involvement of MMPs in this process concerning to the development of the small intestine (Kurakata et al., 2008; Camargo et al., 2016) and none report regarding ex...

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MT1-MMP expression in the odontogenic region of rat incisors undergoing interrupted eruption

Cited by 16 publications

References 35 publications

Relationship Between Cell Proliferation and Eruption Rate in the Rat Incisor

Relationship Between Cell Proliferation and Eruption Rate in the Rat Incisor

Epigenetic regulation of matrix metalloproteinase expression in ameloblastoma

Are the Matrix Metalloproteinases 2 and 9 beeing expressed in the mucosa of the rat small intestine during its intrauterine and postnatal life development ?

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