MTA1 promotes cell proliferation via DNA damage repair in epithelial ovarian cancer

Yang, Qingyuan; Li, J H; Wang, Q Y; Wu, Yukang; Qin, Jinlong; Cheng, Jiajing; Qiu, Jin

doi:10.4238/2014.december.4.21

Cited by 7 publications

(7 citation statements)

References 23 publications

(27 reference statements)

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“… 13 , 14 Recent studies have identified that MTA1 inhibited differentiation and promoted proliferation in cancer cell lines. 3 , 15 , 16 Though these studies have shown that MTA1 is involved in cancers, the precise role of it in cancers including gastric cancer is not well understood.…”

Section: Discussionmentioning

confidence: 99%

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MTA1 promotes proliferation and invasion in human gastric cancer cells

Yuan¹,

Feng²,

Xiao³

et al. 2015

OTT

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Although metastasis-associated protein 1 (MTA1) has been widely linked to tumor metastasis, the relevant mechanisms remain to be elucidated, especially in gastric cancer. The aim of this study was to examine whether the MTA1 gene is associated with the process of proliferation and invasion by regulating several molecular targets in gastric cancer. MTA1 expression in 61 gastric cancer tissue and adjacent noncancerous tissues was analyzed by immunohistochemistry. The prognostic value of MTA1 for overall survival and disease-free survival was determined by Kaplan–Meier estimates, and the significance of differences between curves was evaluated by the log-rank test. Furthermore, overexpression of MTA1 in SGC7901 and BGC823 cells promoted cell cycle progression, cell adhesion, and cell invasion. Our study found that MTA1 is overexpressed in gastric cancers, which contributes to malignant cell growth by facilitating cell cycle progression through upregulation of cyclin D1 and accelerates the migration and invasion of human gastric cancer cells by regulating expression of fibronectin and MMP2/MMP9. Taken together, MTA1 was involved in the pathogenesis of gastric cancer and might be a candidate therapeutic target in gastric cancer.

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Section: Discussionmentioning

confidence: 99%

“… 22 Furthermore, MTA1 promotes cell proliferation by enhancing DNA repair in epithelial ovarian cancer cells. 15 …”

Section: Discussionmentioning

confidence: 99%

MTA1 promotes proliferation and invasion in human gastric cancer cells

Yuan¹,

Feng²,

Xiao³

et al. 2015

OTT

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“…Redox-sensitive proteins also show the influence on cell cycle progression [[78], [79], [80]]. Some authors reported that the cell proliferation can be promoted by enhancing DNA repair [81,82]. Therefore, some of the modulatory effects on DNA damage and repair under the influence if vitamin D 3 and its derivatives might occur as a function of proliferative status rather than induction of stress response pathways.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of novel derivatives of vitamin D3 and lumisterol against UVB-induced damage in human keratinocytes involve activation of Nrf2 and p53 defense mechanisms

et al. 2019

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We tested whether novel CYP11A1-derived vitamin D 3 - and lumisterol-hydroxyderivatives, including 1,25(OH) 2 D 3 , 20(OH)D 3 , 1,20(OH) 2 D 3 , 20,23(OH) 2 D 3 , 1,20,23(OH) 3 D 3 , lumisterol, 20(OH)L 3 , 22(OH)L 3 , 20,22(OH) 2 L 3 , and 24(OH)L 3 , can protect against UVB-induced damage in human epidermal keratinocytes. Cells were treated with above compounds for 24 h, then subjected to UVB irradiation at UVB doses of 25, 50, 75, or 200 mJ/cm 2 , and then examined for oxidant formation, proliferation, DNA damage, and the expression of genes at the mRNA and protein levels. Oxidant formation and proliferation were determined by the DCFA-DA and MTS assays, respectively. DNA damage was assessed using the comet assay. Expression of antioxidative genes was evaluated by real-time RT-PCR analysis. Nuclear expression of CPD, phospho-p53, and Nrf2 as well as its target proteins including HO-1, CAT, and MnSOD, were assayed by immunofluorescence and western blotting. Treatment of cells with the above compounds at concentrations of 1 or 100 nM showed a dose-dependent reduction in oxidant formation. At 100 nM they inhibited the proliferation of cultured keratinocytes. When keratinocytes were irradiated with 50–200 mJ/cm 2 of UVB they also protected against DNA damage, and/or induced DNA repair by enhancing the repair of 6-4PP and attenuating CPD levels and the tail moment of comets. Treatment with test compounds increased expression of Nrf2-target genes involved in the antioxidant response including GR , HO-1 , CAT , SOD1, and SOD2 , with increased protein expression for HO-1, CAT, and MnSOD. The treatment also stimulated the phosphorylation of p53 at Ser-15, increased its concentration in the nucleus and enhanced Nrf2 translocation into the nucleus. In conclusion, pretreatment of keratinocytes with 1,25(OH) 2 D 3 or CYP11A1-derived vitamin D 3 - or lumisterol hydroxy-derivatives, protected them against UVB-induced damage via activation of the Nrf2-dependent antioxidant response and p53-phosphorylation, as well as by the induction of the DNA repair system. Thus, the new vitamin D 3 and lumisterol hydroxy-derivatives represent promising anti-photodamaging agents.

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“…Redox-sensitive proteins also show the influence on cell cycle progression [78][79][80]. Some authors reported that the cell proliferation can be promoted by enhancing DNA repair [81,82]. Therefore, some of the modulatory effects on DNA damage and repair under the influence if vitamin D 3 and its derivatives might occur as a function of Fig.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of novel derivatives of vitamin D3 and lumisterol against UVB-induced damage in human keratinocytes involve activation of Nrf2 and P53 defense mechanisms

Chaiprasongsuk

Kim

Holick

et al. 2018

Free Radical Biology and Medicine

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We tested whether novel CYP11A1-derived vitamin D 3-and lumisterol-hydroxyderivatives, including 1,25(OH) 2 D 3 , 20(OH)D 3 , 1,20(OH) 2 D 3 , 20,23(OH) 2 D 3 , 1,20,23(OH) 3 D 3 , lumisterol, 20(OH)L 3 , 22(OH)L 3 , 20,22(OH) 2 L 3 , and 24(OH)L 3 , can protect against UVB-induced damage in human epidermal keratinocytes. Cells were treated with above compounds for 24 h, then subjected to UVB irradiation at UVB doses of 25, 50, 75, or 200 mJ/cm 2 , and then examined for oxidant formation, proliferation, DNA damage, and the expression of genes at the mRNA and protein levels. Oxidant formation and proliferation were determined by the DCFA-DA and MTS assays, respectively. DNA damage was assessed using the comet assay. Expression of antioxidative genes was evaluated by real-time RT-PCR analysis. Nuclear expression of CPD, phospho-p53, and Nrf2 as well as its target proteins including HO-1, CAT, and MnSOD, were assayed by immunofluorescence and western blotting. Treatment of cells with the above compounds at concentrations of 1 or 100 nM showed a dose-dependent reduction in oxidant formation. At 100 nM they inhibited the proliferation of cultured keratinocytes. When keratinocytes were irradiated with 50-200 mJ/cm 2 of UVB they also protected against DNA damage, and/or induced DNA repair by enhancing the repair of 6-4PP and attenuating CPD levels and the tail moment of comets. Treatment with test compounds increased expression of Nrf2-target genes involved in the antioxidant response including GR, HO-1, CAT, SOD1, and SOD2, with increased protein expression for HO-1, CAT, and MnSOD. The treatment also stimulated the phosphorylation of p53 at Ser-15, increased its concentration in the nucleus and enhanced Nrf2 translocation into the nucleus. In conclusion, pretreatment of keratinocytes with 1,25(OH) 2 D 3 or CYP11A1-derived vitamin D 3-or lumisterol hydroxy-derivatives, protected them against UVB-induced damage via activation of the Nrf2-dependent antioxidant response and p53-phosphorylation, as well as by the induction of the DNA repair system. Thus, the new vitamin D 3 and lumisterol hydroxy-derivatives represent promising anti-photodamaging agents.

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MTA1 promotes cell proliferation via DNA damage repair in epithelial ovarian cancer

Cited by 7 publications

References 23 publications

MTA1 promotes proliferation and invasion in human gastric cancer cells

MTA1 promotes proliferation and invasion in human gastric cancer cells

Protective effects of novel derivatives of vitamin D3 and lumisterol against UVB-induced damage in human keratinocytes involve activation of Nrf2 and p53 defense mechanisms

Protective effects of novel derivatives of vitamin D3 and lumisterol against UVB-induced damage in human keratinocytes involve activation of Nrf2 and P53 defense mechanisms

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