MTERFD1 functions as an oncogene

Zhang, Chaoxiong; Wu, Ning; Gao, Fu Sheng; Han, Jiaqi; Yang, Yanyong; Zhou, Chunyun; Sun, Weimin; Xian, Linfeng; Cheng, Ying; Li, Bailong; Cai, Jianming; Liu, Cong

doi:10.18632/oncotarget.11140

Cited by 2 publications

(10 citation statements)

References 32 publications

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“…Although regulation of mitochondrial genes by MTERFD1 has been thoroughly investigated 8, 9 , the role of MTERFD1 in tumorigenesis has been unreported until recently 10. MTERFD1's role in CRC remains uncertain.…”

Section: Discussionmentioning

confidence: 99%

“…The MTERFD1 overexpression (MTERFD1-OE) plasmid (pcDNA3.1-MTERFD1) was designed and constructed as described previously 10. The blank plasmid (pcDNA3.1) was used as a control for cell transfection.…”

Section: Methodsmentioning

confidence: 99%

“…Gene amplification and upregulation of MTERFD1 were negatively correlated with overall patient survival. MTERFD1 overexpression enhanced tumor cell growth in vivo and in vitro 10. However, the oncogenic effect of MTERFD1 in CRC remains uncertain, and the underlying molecular mechanism of MTERFD1 remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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MTERFD1 promotes cell growth and irradiation resistance in colorectal cancer by upregulating interleukin-6 and interleukin-11

et al. 2019

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The role of the novel oncogene, mitochondrial transcription termination factor (MTERFD1), in human colorectal cancer (CRC) is unclear. Here, we report the role MTERFD1 in CRC. We conducted plasmid construction and transfection analyses, cell proliferation assays, apoptosis detection assays, ELISA, western blotting, and qRT-PCR using cell culture applications. MTERFD1 was upregulated in human and chemically induced mouse CRC tissues. In vitro functional assays showed that MTERFD1 overexpression promoted human CRC cell proliferation, whereas knockdown of endogenous MTERFD1 significantly enhanced apoptosis in these cells. MTERFD1 expression was positively linked to irradiation resistance in CRC cells. Furthermore, interleukin (IL)-6 and IL-11 were identified as the effector molecules of MTERFD1 in its oncogenic role and irradiation resistance in CRC cells. Our results demonstrated that MTERFD1 played an oncogenic role in CRC development and enhanced irradiation resistance by regulating IL-6 and IL-11 in CRC cells. MTERFD1 may serve as a potential prognostic and therapeutic marker for radiotherapy in CRC.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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MTERFD1 promotes cell growth and irradiation resistance in colorectal cancer by upregulating interleukin-6 and interleukin-11

et al. 2019

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“…The inactivation of MTERF3 in the myocardium and skeletal muscle tissue may lead to abnormal mtDNA transcription, resulting in severe respiratory chain defects and decreased oxidative phosphorylation ( 15 ). MTERF3 gene amplification and overexpression are common in various types of solid tumors ( 16 ). In addition, abnormal protein expression of MTERF3 may be involved in the occurrence, development, and metastasis of malignant tumors ( 16 - 18 ).…”

Section: Introductionmentioning

confidence: 99%

“…MTERF3 gene amplification and overexpression are common in various types of solid tumors ( 16 ). In addition, abnormal protein expression of MTERF3 may be involved in the occurrence, development, and metastasis of malignant tumors ( 16 - 18 ). However, the role of MTERF3 gene in breast cancer has remained elusive.…”

Section: Introductionmentioning

confidence: 99%

Expression of MTERF3 gene in breast carcinoma and the relationship with clinicopathological characteristics

Wang¹,

Zi²,

Sun³

et al. 2020

Transl Cancer Res TCR

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Background Mitochondrial transcription termination factor 3 (MTERF3) is a negative regulator of mitochondrial transcription. It is a modular factor involves in mitochondrial ribosome biogenesis and protein synthesis. However, the association between MTERF3 and breast cancers remains largely unknown. The aim of this study was to investigate the expression of MTERF3 in breast carcinoma and to analyze its clinicopathological significance, and to examine the potential prognostic value of MTERF3 in breast cancer. Methods The protein expression levels of MTERF3 in MCF7 (Luminal A), BT-474 (Luminal B), SKBR3 (HER2 overexpression), MDA-MB-468 (Basal like) and MCF10A cell lines were detected by Western blotting. Immunohistochemistry (IHC), Western blotting, and semiquantitative RT-PCR were performed to analyze the protein and mRNA expression levels of MTERF3 in 58 breast cancer tissues and 58 noncancerous breast tissues. The MTERF3 expression data and clinical information from breast cancer patients were downloaded from the TCGA dataset by using the R3.6.1 software. Then the relationship between the expression level of MTERF3 and clinicopathological characteristics and the prognostic value was analyzed. A Cox regression model was performed for the multivariate analysis of the factors that affected the prognosis of breast cancer. The association between the expression levels of MTERF3 and other mitochondrial regulatory genes was analyzed with GEPIA. Results MTERF3 is upregulated in breast cancer cell lines compared to noncancerous breast cell line. The IHC results showed that the MTERF3 protein was located in the cytoplasm, and the rate of positive expression in breast cancer tissue was significantly upregulated compared with the adjacent normal tissue. The mRNA and protein expression levels of MTERF3 in breast cancer tissues were significantly higher than that in breast tissues. Moreover, the expression of MTERF3 was significantly correlated with ER status, PR status, breast cancer molecular typing, cancer type, histological diagnosis and primary site (P<0.05). Further analysis showed MTERF3 expression was not related to prognosis. Multivariate Cox regression analysis showed that age, metastasis status and tumor type were independent prognostic factors for breast cancer patients. The expression levels of MTERF3 were positively correlated with the TFAM , TFB1M , TFB2M , MTERF1 , TEFM and MFN1 genes but negatively correlated with the MTERF4 and PINK1 genes. In addition, the expression levels of MTERF3 were not correlated with the MTERF2 gene. Conclusions MTERF3 was significantly upregulated in breast cancer cells and tissues compared with noncancerous cells and tissues. Moreover, th...

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MTERFD1 functions as an oncogene

Cited by 2 publications

References 32 publications

MTERFD1 promotes cell growth and irradiation resistance in colorectal cancer by upregulating interleukin-6 and interleukin-11

MTERFD1 promotes cell growth and irradiation resistance in colorectal cancer by upregulating interleukin-6 and interleukin-11

Expression of MTERF3 gene in breast carcinoma and the relationship with clinicopathological characteristics

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