MTNR1A gene polymorphisms and reproductive recovery after seasonal anoestrus in different Mediterranean sheep breeds

Pulinas, Luisa; Starič, Jože; Cosso, Giovanni; Curone, Giulio; Mura, Maria Consuelo; Carcangiu, Vincenzo; Luridiana, Sebastiano

doi:10.1016/j.anireprosci.2021.106905

Cited by 10 publications

(17 citation statements)

References 31 publications

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“…The two SNPs, rs430181568 and rs407388227, as reported in several studies on both the Sarda and other breeds [ 13 , 25 ], are associated with the regulation of reproductive seasonality. The SNP rs407388227 causes an amino acid change (Val > Ile) at position 220 of the protein chain (GenBank access number AAB17721.1).…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we also analyzed exon 1, but no polymorphism was found there. Furthermore, we studied the relationship between the found polymorphisms in exon 2 with reproductive activity in Sarda and in other sheep breeds, and only rs430181568 and rs407388227 showed a statistically significant association [ 7 , 13 , 18 , 24 , 25 ]. Thus, as these topics were extensively discussed, in this study, we focused only on the rs430181568 and rs407388227 SNPs.…”

Section: Discussionmentioning

confidence: 99%

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Reproductive Resumption in Winter and Spring Related to MTNR1A Gene Polymorphisms in Sarda Sheep

Mura

Cosso

Pulinas

et al. 2022

Animals

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The aim of the present research was to evaluate the association between the SNPs rs430181568 and rs407388227 located in the MTNR1A gene with the reproductive recovery of Sarda sheep in different months of ram introduction in the flock (February, March, April and May). In order to address this research gap, we selected two farms, each of which consisted of approximately 1000 animals; a total of 800 ewes (400 for each farm) were genotyped for the two single nucleotide polymorphisms rs430181568 and rs407388227 located in the exon 2 of the MTNR1A. These SNPs are completely linked; thus, each genotype of rs430181568 corresponded to the same genotype for rs407388227. Among the genotyped animals, 240 individuals were selected and divided into four homogeneous groups (A, B, C and D) of 60 subjects, each group based on age (range 3–6 years old), body condition score (BCS) (range 2.0–4.0) and genotype (20 ewes carrying CC/CC, 20 CT/CT and 20 TT/TT genotype). The dates of the ram introduction in each group were 15 February, 15 March, 15 April and 15 May, respectively. In all groups, the lambing date and the number of lambs born from 150 to 220 days after the ram introduction were recorded. In all the groups, the genotypes CC/CC and CT/CT of the polymorphism (rs430181568 and rs407388227) showed the greatest fertility (the ratio between the number of lambed ewes and the ewes exposed to the rams) (p < 0.01) and the shortest distance between ram introduction to lambing (p < 0.01), compared with the TT/TT genotype. In conclusion, we determined that the polymorphisms rs430181568 and rs407388227 were associated with reproductive recovery, after ram introduction, even in February and March, months subsequent to the photorefractoriness period.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Reproductive Resumption in Winter and Spring Related to MTNR1A Gene Polymorphisms in Sarda Sheep

Mura

Cosso

Pulinas

et al. 2022

Animals

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“…Ewes mating to rams or other ewes had the same results. 13 This was the case regardless of whether the ewes were mated to rams or other ewes.…”

Section: Mtnr1a Role By Seasonmentioning

confidence: 97%

“… 5 , 22 Despite the fact that the ramifications of the MTNR1A gene on reproductive seasonality have only been investigated to a limited extent up until this point, those studies have shown that these effects are tied to three specific mutations discovered in exon II at locations 606, 612, and 706, 7 , 21 only sheep of the Sarda and Aragonese breeds have had the remaining portions of this gene examined. 12 , 13 , 23 – 25 …”

Section: Mtnr1a Locationmentioning

confidence: 99%

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Association of melatonin receptor 1 A with litter size in sheep: A review

Abuzahra,

Wijayanti,

Effendi

et al. 2024

F1000Res

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Sheep are a valuable livestock species worldwide, providing meat, milk, and various dairy products. This article aims to review the latest literature on the melatonin receptor 1A (MTNR1A) gene as a potential candidate gene associated with reproductive traits, particularly the litter size trait in sheep, by searching various databases for available literature. Studies have shown that different parts of the MTNR1A gene play various roles in sheep. By identifying marker genes associated with reproductive traits in MTNR1A polymorphisms linked to the litter size trait, breeders can achieve a faster selection response in sheep breeding by recognizing the genomic region where these genes are located and understanding their physiological functions. Therefore, highlighting the literature on these functions and their association with reproductive traits may contribute to improving the genetic makeup during sheep breeding.

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Differential effect of melatonin on ram spermatozoa depending on the allelic variant of the RsaI polymorphism of the MTR1A gene, incubation medium and season

Peña-Delgado,

Noya,

Carvajal-Serna

et al. 2024

Reprod. Fertil. Dev.

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Context The RsaI polymorphism of the melatonin receptor MTNR1A gene affects seasonal reproduction in sheep, but its effect on ram spermatozoa and their response to melatonin is unknown. Aims This study aims to evaluate whether RsaI polymorphism of the MTNR1A gene influences the response of ram spermatozoa to in vitro added melatonin. Methods Spermatozoa from rams carrying different RsaI allelic variants were incubated with melatonin in a TALP medium or a capacitation-triggering medium during the reproductive and non-reproductive seasons. After incubation, sperm motility, membrane integrity, mitochondria activity, oxidative damage, apoptotic markers and capacitation status were assessed. Key results In the reproductive season, the T/T genotype was related to some adverse effects of melatonin when spermatozoa were incubated in TALP medium, whereas the C/C genotype was linked with adverse effects when the hormone was added in a capacitation-triggering medium. The decapacitating effect of melatonin on spermatozoa was also different depending on genotype. Conclusions The melatonin effect on spermatozoa from rams carrying different RsaI genotypes differed depending on the season and the medium. Implications The knowledge of the RsaI allelic variant of the MTNR1A gene of rams could be helpful when carrying out in vitro reproductive techniques in the ovine species.

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MTNR1A gene polymorphisms and reproductive recovery after seasonal anoestrus in different Mediterranean sheep breeds

Cited by 10 publications

References 31 publications

Reproductive Resumption in Winter and Spring Related to MTNR1A Gene Polymorphisms in Sarda Sheep

Reproductive Resumption in Winter and Spring Related to MTNR1A Gene Polymorphisms in Sarda Sheep

Association of melatonin receptor 1 A with litter size in sheep: A review

Differential effect of melatonin on ram spermatozoa depending on the allelic variant of the RsaI polymorphism of the MTR1A gene, incubation medium and season

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