MTO1 Worked as a Modifier in the Aminoglycosides Sensitivity of Yeast Carrying a Mitochondrial 15S rRNA C1477G Mutation

Zhu, Xiaoyu; He, Xiuping; Wang, Wei; Zhou, Qing; Yu, Zhe; Dai, Yu; Zhu, Xuemei; Yan, Qingfeng

doi:10.1371/journal.pone.0124200

Cited by 5 publications

(4 citation statements)

References 26 publications

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“…A host of other mitochondrial genes and variants have also been demonstrated to confer risk to aminoglycoside‐induced HL 56 . Many nuclear genes can modify the severity of amingolycoside‐A1555G interactions, including MTTS1 , TFBM1 , and GTPBP3 4,57–59 . The maternal inheritance of mitochondria could guide practitioners to avoid use of aminoglycosides in patients with maternal history of HL after aminoglycoside exposure.…”

Section: Ototoxin Susceptibilitymentioning

confidence: 99%

Genetics of Postlingual Sensorineural Hearing Loss

et al. 2020

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Literature and clinical practice around adult‐onset hearing loss (HL) has traditionally focused on environmental risk factors, including noise exposure, ototoxic drug exposure, and cardiovascular disease. The most common diagnosis in adult‐onset HL is presbycusis. However, the age of onset of presbycusis varies, and patients often describe family history of HL as well as individual variation in progression and severity. In recent years, there has been accumulating evidence of gene–environment interactions underlying adult cases of HL. Susceptibility loci for age‐related HL have been identified, and genes related to postlingual nonsyndromic HL continue to be discovered through individual reports and genome‐wide association studies. This review will outline main concepts in genetics as related to HL, identify implicated genes, and discuss clinical implications. Laryngoscope, 131:401–409, 2021

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Section: Ototoxin Susceptibilitymentioning

confidence: 99%

Genetics of Postlingual Sensorineural Hearing Loss

et al. 2020

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“…Basal respiration was evaluated by the oxygen consumption rate (OCR) measurements carried out as previously described with minor modifications 61 . Yeast cells were cultured overnight at 30 °C in SC medium.…”

Section: Methodsmentioning

confidence: 99%

Rapid deacetylation of yeast Hsp70 mediates the cellular response to heat stress

Nitika

Hasin

et al. 2019

Sci Rep

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Hsp70 is a highly conserved molecular chaperone critical for the folding of new and denatured proteins. While traditional models state that cells respond to stress by upregulating inducible HSPs, this response is relatively slow and is limited by transcriptional and translational machinery. Recent studies have identified a number of post-translational modifications (PTMs) on Hsp70 that act to fine-tune its function. We utilized mass spectrometry to determine whether yeast Hsp70 (Ssa1) is differentially modified upon heat shock. We uncovered four lysine residues on Ssa1, K86, K185, K354 and K562 that are deacetylated in response to heat shock. Mutation of these sites cause a substantial remodeling of the Hsp70 interaction network of co-chaperone partners and client proteins while preserving essential chaperone function. Acetylation/deacetylation at these residues alter expression of other heat-shock induced chaperones as well as directly influencing Hsf1 activity. Taken together our data suggest that cells may have the ability to respond to heat stress quickly though Hsp70 deacetylation, followed by a slower, more traditional transcriptional response.

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“…GidA and MnmE, two important tRNA modification enzymes, are involved in the addition of the cmnm group in the position 5 of the wobble uridine 34 of tRNAs that read codons ending with A or G (Yim et al, 2006;Shippy and Fadl, 2015;Gao et al, 2019). Correspondingly, the orthologs of GidA and MnmE are called MTO1 and MSS1/GTPBP3 in yeast and human respectively (Zhu et al, 2015). There are three main structural or functional roles for this modification: (i) proper codon-anticodon interaction at the decoding center of the ribosome (Fislage et al, 2014), (ii) appropriate decoding of mRNA (Urbonavicius et al, 2001), (iii) maintaining the translational reading frame (Tükenmez et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Proteomic and Metabolomic Analyses Provide Insights into the Mechanism on Arginine Metabolism Regulated by tRNA Modification Enzymes GidA and MnmE of Streptococcus suis

Gao¹,

Yuan²,

Liu³

et al. 2020

Front. Cell. Infect. Microbiol.

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GidA and MnmE, two important tRNA modification enzymes, are contributed to the addition of the carboxymethylaminomethyl (cmnm) group onto wobble uridine of tRNA. GidA-MnmE modification pathway is evolutionarily conserved among Bacteria and Eukarya, which is crucial in efficient and accurate protein translation. However, its function remains poorly elucidated in zoonotic Streptococcus suis (SS). Here, a gidA and mnmE double knock-out (DKO) strain was constructed to systematically decode regulatory characteristics of GidA-MnmE pathway via proteomic. TMT labelled proteomics analysis identified that many proteins associated with cell divison and growth, fatty acid biosynthesis, virulence, especially arginine deiminase system (ADS) responsible for arginine metabolism were down-regulated in DKO mutant compared with the wild-type (WT) SC19. Accordingly, phenotypic experiments showed that the DKO strain displayed decreased in arginine consumption and ammonia production, deficient growth, and attenuated pathogenicity. Moreover, targeted metabolomic analysis identified that arginine was accumulated in DKO mutant as well. Therefore, these data provide molecular mechanisms for GidA-MnmE modification pathway in regulation of arginine metabolism, cell growth and pathogenicity of SS. Through proteomic and metabolomic analysis, we have identified arginine metabolism that is the links between a framework of protein level and the metabolic level of GidA-MnmE modification pathway perturbation.

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MTO1 Worked as a Modifier in the Aminoglycosides Sensitivity of Yeast Carrying a Mitochondrial 15S rRNA C1477G Mutation

Cited by 5 publications

References 26 publications

Genetics of Postlingual Sensorineural Hearing Loss

Genetics of Postlingual Sensorineural Hearing Loss

Rapid deacetylation of yeast Hsp70 mediates the cellular response to heat stress

Proteomic and Metabolomic Analyses Provide Insights into the Mechanism on Arginine Metabolism Regulated by tRNA Modification Enzymes GidA and MnmE of Streptococcus suis

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