2018
DOI: 10.1016/j.cell.2018.05.042
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mTORC1 Controls Phase Separation and the Biophysical Properties of the Cytoplasm by Tuning Crowding

Abstract: Highlights d GEMs enable high-throughput microrheology in unperturbed living cells d mTORC1 controls diffusion by tuning ribosome concentration d Diffusion can be accurately predicted as a function of ribosome concentration d Crowding of the cytoplasm by ribosomes increases phase separation

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Cited by 415 publications
(513 citation statements)
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“…We found that average GEM diffusivity was 0.02±0.02 (SD) µm 2 s −1 (Fig. 3C), slower than previously reported for the same particles in the budding yeast S. cerevisiae (5). Our diffusivity is underestimated due to the limitations of 3D imaging rate, but direct comparisons between the species in identical imaging conditions also show that diffusivity in A. gossypii is ∼2-fold slower than S. cerevisiae (G.P.…”
Section: Testing Inference Accuracy Using Synthetic Videossupporting
confidence: 42%
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“…We found that average GEM diffusivity was 0.02±0.02 (SD) µm 2 s −1 (Fig. 3C), slower than previously reported for the same particles in the budding yeast S. cerevisiae (5). Our diffusivity is underestimated due to the limitations of 3D imaging rate, but direct comparisons between the species in identical imaging conditions also show that diffusivity in A. gossypii is ∼2-fold slower than S. cerevisiae (G.P.…”
Section: Testing Inference Accuracy Using Synthetic Videossupporting
confidence: 42%
“…1). Multiple studies of liquid-liquid phase separation (5,(10)(11)(12) showed that phase separation can be enhanced by crowding, and we speculated that heterogeneity in crowding can influence where biomolecular condensates form.…”
mentioning
confidence: 92%
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“…It is becoming increasingly clear that excluded volume effects mediated by molecular crowding affect macromolecular structure, protein stability, enzyme activity and nucleo-cytoplasmic organization (Daher et al, 2018;Delarue et al, 2018;Hancock, 2004;Minton, 2001;Sukenik et al, 2018). Previous work has noted the potential for phase separation to dynamically buffer the intracellular protein concentration (Alberti et al, 2019).…”
Section: The Features and Functional Consequences Of Widespread Intramentioning
confidence: 99%