MTT Assay on Anticancer properties of the different extracts of roots of Lantana camara Linn.

Abstract

Lantana camara, considered a weed is an evergreen plant found throughout the world. Traditionally it has been used in the treatment of various ailments and has been supported by scientific investigation. Phytoconstituents present in almost all parts of this plant have been reported in various literature. This study has been done on the root part of Lantana camara, the dried and powdered root was subjected to continuous solvent extraction with Hexane followed by Ethyl Acetate and Methanol. Ethyl Acetate and Methanol extracts were evaluated for anticancer activity by MTT assay, MCF7 cell line was used with Cisplatin a known anti-cancer drug as a standard reference, and the measurements were carried out at wavelengths 570 and 630nm. The percentage of growth inhibition was calculated. The concentration of test extract needed to inhibit cell growth by 50% (IC50) value generated from the dose-response curve. IC50 values of Ethyl Acetate extract were greater than 500µg/ml and Methanol extract 450.05µg/ml.