MUC13, a Novel Human Cell Surface Mucin Expressed by Epithelial and Hemopoietic Cells

Williams, Stephanie J.; Wreschner, Daniel H.; Tran, Minh; Eyre, Helen J.; Sutherland, Grant R.; McGuckin, Michael A.

doi:10.1074/jbc.m008850200

Cited by 280 publications

(258 citation statements)

References 34 publications

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“…Thirteen human mucins have been cloned or partially cloned to date (MUC-1, -2, -3, -4, -5AC, -5B, -6, -7, -8, -9, -11, -12 and -13; Refs. [23][24][25][26][27][28][29]. In this study, however, a polyclonal antiserum to the native mucin was used to isolate a cDNA corresponding to the peptide moiety of CA125/MUC16 antigen.…”

Section: Resultsmentioning

confidence: 99%

Molecular Cloning of the CA125 Ovarian Cancer Antigen

Yin

Lloyd

2001

Journal of Biological Chemistry

552

404

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CA125 is an ovarian cancer antigen that is the basis for a widely used serum assay for the monitoring of patients with ovarian cancer; however, detailed information on its biochemical and molecular nature is lacking. We now report the isolation of a long, but partial, cDNA that corresponds to the CA125 antigen. A rabbit polyclonal antibody produced to purified CA125 antigen was used to screen a ZAP cDNA library from OVCAR-3 cells in Escherichia coli. The longest insert from the 54 positive isolated clones had a 5797-base pair sequence containing a stop codon and a poly(A) sequence but no clear 5 initiation sequence. The deduced amino acid sequence has many of the attributes of a mucin molecule and was designated CA125/MUC16 (gene MUC16). These features include a high serine, threonine, and proline content in an N-terminal region of nine partially conserved tandem repeats (156 amino acids each) and a C-terminal region non-tandem repeat sequence containing a possible transmembrane region and a potential tyrosine phosphorylation site. Northern blotting showed that the level of MUC16 mRNA correlated with the expression of CA125 in a panel of cell lines. The molecular cloning of the CA125 antigen will lead to a better understanding of its role in ovarian cancer.

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Section: Resultsmentioning

confidence: 99%

Molecular Cloning of the CA125 Ovarian Cancer Antigen

Yin

Lloyd

2001

Journal of Biological Chemistry

552

404

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“…However, using this same oligonucleotide we were unable to detect any differences between PMA-treated and -untreated cells. 2 It is possible that this region of the promoter is important but that PMAinduced up-regulation involves increased transcriptional activity of Sp1 or other nuclear factors that are already bound to this proximal region of the MUC2 promoter. Further experiments are required to identify the PMA-responsive, ERK-activated MUC2 promoter elements.…”

Section: Discussionmentioning

confidence: 99%

“…They are synthesized by the epithelial cells lining the gastrointestinal, respiratory, and genitourinary tracts. Genomic and cDNA sequencing has identified at least fifteen different mucin genes, which encode either secretory or membrane-associated proteins (1)(2)(3). Mucins are expressed in a characteristic tissue-and cell type-specific manner.…”

mentioning

confidence: 99%

Phorbol 12-Myristate 13-Acetate Up-regulates the Transcription of MUC2Intestinal Mucin via Ras, ERK, and NF-κB

Lee

Ahn

Crawley

et al. 2002

Journal of Biological Chemistry

103

108

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“…Their main functions are thought to be lubrication and protection of the epithelial surface [1,2] . To date, at least thirteen mucins have been found [3] . MUC1, an epithelial mucin glycoprotein, is highly expressed in lactating mammary glands [4] .…”

Section: Introductionmentioning

confidence: 99%

Expression of mucins and E-cadherin in gastric carcinoma and their clinical significance

Zhang

Zhang²,

Zhao³

et al. 2004

WJG

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AIM:To investigate the expression of three types of mucin (MUC1, MUC2, MUC5AC) and E-cadherin in human gastric carcinomas and their clinical significance. METHODS:Ninety-four gastric cancer specimens were classified according to WHO criteria and detected by immunohistochemical assay of expression of mucins and E-cadherin. RESULTS:The positive expression rates of MUC1, MUC2, MUC5AC and E-cadherin were 82% (77/94), 84% (79/94), 40% (38/94) and 56% (53/94) respectively. MUC1 expression was significantly correlated with the types of cancer (the positive rates of MUC1 in well and moderately differentiated tubular adenocarcinoma, poorly differentiated adenocarcinoma, signet-ring cell carcinoma and mucinous carcinoma were 91%, 87%, 71%, 71%, respectively, P<0.05), age of patients (the positive rates of it among the people who are younger than 40 years, between 40-60 years and over 60 year were 74%, 81%, 89%, P<0.05), lymph nodes involvement (the positive rates in the non-interfered group and the interfered group were 78%, 85%, P<0.05) and tumor size (the positive rates in the tumors with the size less than 3 cm, 3-6 cm and larger than 6 cm were 69%, 92%, 69%, P<0.05); MUC2 expression was significantly associated with types of cancers and had the strongest expression in mucinous carcinomas (the positive rates of MUC2 in well and moderately differentiated tubular adenocarcinoma, poorly differentiated adenocarcinoma, signet-ring cell carcinoma and mucinous carcinoma were 94%, 70%, 81%, 100%, P<0.05), but it had no obvious relation to age, gender, tumor location, lymph nodes involvement, depth of invasion and metastasis to extra-gastric organs (P>0.05); MUC5AC expression was not related to any of the characteristics investigated except that it had relation to gender, whereas MUC5AC showed the tendency to higher expression in less invasive lesions and lower expression in advanced stage cancers (P>0.05); No significant difference was found for E-cadherin expression. There were strong positive relationships between the expression of MUC1 and E-cadherin, MUC2 and E-cadherin, MUC1 and MUC2 (R = 0.33, R = 0.22, R = 0.32, respectively, P<0.05). According to the COX proportional hazards model, older patients, involvement of lymph nodes, different types of gastric cancer and MUC2 expression were significantly associated with poorer outcome of gastric carcinoma patients (β = 0.08, β = 3.94, β = 1.33, β = 0.75, respectively, P<0.05).CONCLUSION: MUC1 and MUC2 are good markers of different types of gastric cancer. MUC2 is especially a good marker of mucinous carcinoma. MUC1, MUC2 may interfere with the function of E-cadherin in gastric carcinomas, and have synergic effect on progression of gastric cancers.Zhang HK, Zhang QM, Zhao TH, Li YY, Yi YF. Expression of mucins and E-cadherin in gastric carcinoma and their clinical significance.

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MUC13, a Novel Human Cell Surface Mucin Expressed by Epithelial and Hemopoietic Cells

Cited by 280 publications

References 34 publications

Molecular Cloning of the CA125 Ovarian Cancer Antigen

Molecular Cloning of the CA125 Ovarian Cancer Antigen

Phorbol 12-Myristate 13-Acetate Up-regulates the Transcription of MUC2Intestinal Mucin via Ras, ERK, and NF-κB

Expression of mucins and E-cadherin in gastric carcinoma and their clinical significance

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