MUC5B Secretion is Up-Regulated in Sinusitis Compared with Controls

Viswanathan, Harishnath; Brownlee, Iain A.; Pearson, Jeffrey P.; Carrie, Sean

doi:10.2500/ajr.2006.20.2935

Cited by 29 publications

(28 citation statements)

References 16 publications

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“…Absorbance was read at 450 nm (Sunrise remote; Tecan). The MUC5AC protein content in each sample was estimated in comparison to a standard mucin (polymeric porcine gastric mucin) as previously described (Ali et al 2002;Viswanathan et al 2006). In groups that provided multiple samples, the mean value of MUC5AC protein content was used in the analysis.…”

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Interleukin-13 Induces Mucin 5AC Production Involving STAT6/SPDEF in Human Airway Epithelial Cells

Кolosov

et al. 2010

Cell Communication & Adhesion

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Mucus hypersecretion is commonly observed in many chronic airway infl ammatory diseases. Mucin 5AC (MUC5AC) is a major airway mucin because of its high expression in goblet cells. Here, the authors identifi ed a gene called SAM domain -containing prostate-derived Ets factor (SPDEF) that was induced by interleukin (IL)-13. Their results showed that specifi c knockdown of SPDEF reduced IL-13-induced MUC5AC expression in human airway epithelial cells. This fi nding was associated with decreased expression of anterior gradient 2 (AGR2) and Ca 2 ϩ -activated Cl Ϫ channel (CLCA1), which regulate IL-13-mediated MUC5AC overproduction. Furthermore, transfection with SPDEF siRNA enhanced expression of forkhead box a2 (Foxa2), a key transcription factor that is known to prevent mucus production. The authors also demonstrated that the repression of STAT6 inhibited expression of SPDEF and MUC5AC induced by IL-13. These results show that SPDEF plays a critical role in regulating a transcriptional network mediating IL-13-induced MUC5AC synthesis dependent on STAT6.

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Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Interleukin-13 Induces Mucin 5AC Production Involving STAT6/SPDEF in Human Airway Epithelial Cells

Кolosov

et al. 2010

Cell Communication & Adhesion

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“…Several of the innate immunity proteins that show increased expression in CRS secretions in our study, e.g., BPIFA1, DMBT1, S100A8, S100A9, LTF, MYH9, and IGHA1, have been reported as being dysregulated in studies of CRS nasal lavage fluid or sinus tissues (6,11,(16)(17)(18)(19). These proteins are discussed below.…”

Section: Discussionmentioning

confidence: 99%

“…To date, there are no studies on MUC5AC and MUC5B levels in sinonasal secretions from children, although MUC5B levels are increased in adult CRS secretions (6). There also appears to be differences in the pathophysiology of CRS in children compared to adults such that results from adult studies may not be applicable to pediatric CRS (7).…”

mentioning

confidence: 95%

Proteomic analysis of pediatric sinonasal secretions shows increased MUC5B mucin in CRS

et al. 2014

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Background: Chronic rhinosinusitis (CRS) is characterized by mucous overproduction and submucosal gland hyperplasia. The global protein profile of sinonasal secretions in pediatric CRS has not been studied. We hypothesized that MUC5B, a glandular mucin, would be relatively increased in CRS secretions compared to other mucins. Methods: Secretions were collected at Children's National Health System (Children's National) from CRS patients undergoing sinus surgery and from control patients without CRS undergoing craniofacial procedures. Proteins were extracted, digested to peptides, and analyzed by mass spectometry. Fold change significance was calculated using the QSpec algorithm. Western blot analysis was performed to validate proteomic findings. results: In total, 294 proteins were identified. Although both MUC5B and MUC5AC were identified in a majority of samples, the relative abundance of MUC5B was found to be significantly higher (P < 0.05). Western blot data validated these findings. Other proteins with the highest significant positive-fold change in CRS samples were BP1 fold-containing family A member 1, chitinase-3-like protein 1, plastin-2, serpin 10, and BP1 fold-containing family B member 1. conclusion: Overall, our data demonstrate an increase of MUC5B abundance in the sinus secretions of pediatric patients with CRS.

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“…29 In general, excessive mucus production is commonly seen in respiratory disease 30 and increased levels of the mucins 5B have been found in persons suffering from chronic rhinosinusitis. 31 Extracellular glycoprotein lacritin was increased in the symptomatic and atopic groups at 20 min. At 5 h, this protein was found at even higher levels in the asymptomatic and atopic groups.…”

Section: Discussionmentioning

confidence: 99%

Time-Dependent Proteomic iTRAQ Analysis of Nasal Lavage of Hairdressers Challenged by Persulfate

et al. 2010

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Hairdressers are frequently exposed to bleaching powder containing persulfates, a group of compounds that may induce hypersensitivity in the airways. The mechanism causing this reaction is not clear. The aim of this study was to identify changes in the nasal lavage fluid proteome after challenge with potassium persulfate in hairdressers with bleaching powder-associated rhinitis. Furthermore, we aimed to compare their response to that of hairdressers without nasal symptoms, and atopic subjects with pollen-associated nasal symptoms. To study the pathogenesis of persulfate-associated rhinitis, the response in protein expression from the upper airway was assessed by time-dependent proteomic expression analysis of nasal lavage fluids. Samples were prepared by pooling nasal lavage fluids from the groups at different time points after challenge. Samples were depleted of high-abundant proteins, labeled with iTRAQ and analyzed by online 2D-nanoLC-MS/MS. Differences in the protein pattern between the three groups were observed. Most proteins with differentially expressed levels were involved in pathways of lipid transportation and antimicrobial activities. The major finding was increased abundance of apolipoprotein A-1, 20 min postchallenge, detected solely in the group of symptomatic hairdressers. Our results suggest there may be differences between the mechanisms responsible for the rhinitis in the symptomatic and atopic group.

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MUC5B Secretion is Up-Regulated in Sinusitis Compared with Controls

Abstract: MUC5B secretion is significantly up-regulated in CRS compared with control subjects (p = 0.04). Correlation between viscosity and mucin content was lost in CRS. This is likely to have important implications for future therapies in CRS.

Cited by 29 publications

References 16 publications

Interleukin-13 Induces Mucin 5AC Production Involving STAT6/SPDEF in Human Airway Epithelial Cells

Interleukin-13 Induces Mucin 5AC Production Involving STAT6/SPDEF in Human Airway Epithelial Cells

Proteomic analysis of pediatric sinonasal secretions shows increased MUC5B mucin in CRS

Time-Dependent Proteomic iTRAQ Analysis of Nasal Lavage of Hairdressers Challenged by Persulfate

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