Mucilaginibacter pedocola sp. nov., isolated from a heavy-metal-contaminated paddy field

Abstract

Strain TBZ30T was isolated from soil of a heavy-metal-contaminated paddy field. Cells of strain TBZ30T were Gram-staining-negative, rod-shaped, non-motile and non-spore-forming. The isolate was strictly aerobic, pink-pigmented, catalase- and oxidase-positive and produced exopolysaccharides. On the basis of 16S rRNA gene phylogeny, strain TBZ30T belonged to the genus Mucilaginibacter and appeared most closely related to Mucilaginibacter gynuensis YC7003T (95.8 %), Mucilaginibacter litoreus BR-18T (95.4 %) and Mucilaginibacter mallensis MP1X4T (95.4 %). Strain TBZ30T contained menaquinone-7 as the only ubiquinone. The main cellular fatty acids included summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), iso-C15 : 0, C16 : 0, iso-C17 : 0 3-OH and C16 : 1ω5c. The polar lipids were phosphatidylethanolamine, an unidentified phospholipid, two unidentified aminophospholipids, four unidentified aminolipids, three unidentified lipids and two unidentified glycolipids. The genomic DNA G+C content was 49.0 mol%. On the basis of polyphasic taxonomy analyses, strain TBZ30T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter pedocola sp. nov. is proposed. The type strain is TBZ30T (=KCTC 42833T=CCTCC AB 2015301T).

Genomic Islands Confer Heavy Metal Resistance in <em>Mucilaginibacter Kameinonensis</em> and <em>Mucilaginibacter Rubeus</em> Isolated from a Gold/Copper Mine

“…Table 2 Minimal inhibitory concentration (MIC) of strains to Zn (II) , As (III) , Cd (II) and Cu (II) Optimal growth occurred in absence of further NaCl, but the strains could still grow in R2A with up to 1.5% NaCl added. These characteristics are consistent with description of the genus Mucilaginibacter [34][35][36] . Medium pH for optimal growth (~pH 5.0) and pH tolerance (pH 5.0-9.0) varied slightly between the four strains ( Table 3).…”

High-quality-draft genome sequence of the heavy metal resistant and exopolysaccharides producing bacterium Mucilaginibacter pedocola TBZ30T

“…Mucilaginibacter pedocola TBZ30 T (= CCTCC AB 2015301 T = KCTC 42833 T ) was isolated from a heavy metal contaminated paddy field in Hunan Province, P. R. China [ 5 ]. Here we show that strain TBZ30 T is resistant to multiple heavy metals and remove Zn 2+ and Cd 2+ .…”

“…Colonies are circular, pink, convex and smooth on R2A agar. Growth occurs aerobically at 4–28 °C (optimum, 25 °C), pH 5.0–8.5 (optimum, pH 7.0), and in the presence of 0–1.0 ( w / v ) NaCl (optimum, without NaCl) (Table 1 ) [ 5 ]. Oxidase- and catalase-positive [ 5 ].…”

“…Growth occurs aerobically at 4–28 °C (optimum, 25 °C), pH 5.0–8.5 (optimum, pH 7.0), and in the presence of 0–1.0 ( w / v ) NaCl (optimum, without NaCl) (Table 1 ) [ 5 ]. Oxidase- and catalase-positive [ 5 ]. It can use glucose, mannose, L-arabinose, maltose, melibiose, rhamnose and glycogen as the sole carbon sources [ 5 ].…”

“…Oxidase- and catalase-positive [ 5 ]. It can use glucose, mannose, L-arabinose, maltose, melibiose, rhamnose and glycogen as the sole carbon sources [ 5 ]. Strain TBZ30 T can produce EPS testing by aniline blue staining method [ 9 ] (Fig.…”