Mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus Mucilaginibacter

Abstract

A non-motile, rod-shaped bacterium, designated strain WPCB133T, was isolated from freshwater collected from the Woopo wetland (Republic of Korea). Cells were Gram-reaction-negative, aerobic and catalase- and oxidase-positive. The major fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The strain contained MK-7 as the major isoprenoid quinone. The DNA G+C content was 47 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WPCB133T forms an independent lineage within the genus Mucilaginibacter. Strain WPCB133T was distantly related to Mucilaginibacter kameinonensis SCKT (94.7 % sequence similarity), Mucilaginibacter paludis TPT56T (94.5 %) and Mucilaginibacter gracilis TPT18T (94.4 %). Phenotypic characteristics distinguished strain WPCB133T from members of the genus Mucilaginibacter. On the basis of evidence presented in this study, strain WPCB133T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter rigui sp. nov. is proposed. The type strain is WPCB133T (=KCTC 12534T =NBRC 101115T). An emended description of the genus Mucilaginibacter is also proposed.

Mucilaginibacter composti sp. nov., with ginsenoside converting activity, isolated from compost

“…It contains menaquinone-7 (MK-7) as the predominant respiratory quinone and unsaturated and branched saturated fatty acids as the major fatty acids. The G+C content of the genomic DNA of this genus ranges from 42.4 to 47.0 mol% (Pankratov et al, 2007;Urai et al, 2008;Baik et al, 2010). At the time of writing, the genus consisted of 13 validly named species, with M. paludis as the type species (Euzéby, 1997).…”

“…The genus Mucilaginibacter was recently proposed by Pankratov et al (2007) and emended by Urai et al (2008) and Baik et al (2010) in order to accommodate Gram-negative, strictly aerobic or facultatively anaerobic, chemoheterotrophic, rod shaped bacteria. It contains menaquinone-7 (MK-7) as the predominant respiratory quinone and unsaturated and branched saturated fatty acids as the major fatty acids.…”

“…The 16S rRNA gene sequences of the strain TR6-03 T determined in this study were continuous stretches of 1480 bp Differentiating characteristics of M. composti TR6-03 T and the type strains of related Mucilaginibacter species Strains: 1, Mucilaginibacter composti TR6-03 T ; 2, Mucilaginibacter lappiensis ANJLI2 T ; 3, Mucilaginibacter rigui WPCB133 T ; 4, Mucilaginibacter dorajii FR-f4 T Data were from this study, Männistö et al (2010), Baik et al (2010), and Kim et al (2010). All strains were Gram-negative, non-motile, rod shaped, positive for oxidase activities.…”

Mucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum

“… Strains: 1, DR‐f4 T ; 2, Mucilaginibacter lappiensis ANJLI2 T ; and 3, Mucilaginibacter rigui WPCB133 T . Data are from this study, Männistö et al (2010) and Baik et al (2010). All strains are nonmotile rods and positive for catalase, oxidase, alkaline phosphatase, leucine arylamidase, valine arylamidase, acid phosphatase, α‐galactosidase, β‐galactosidase, α‐glucosidase, β‐glucosidase, N ‐acetyl‐β‐glucosaminidase, aesculin hydrolysis, assimilation of d ‐glucose, d ‐mannose, N ‐acetyl‐glucosamine and d ‐maltose, and acid production from d ‐arabinose, d ‐galactose, d ‐glucose, d ‐mannose, d ‐cellobiose, d ‐maltose, d ‐lactose and d ‐melibiose.…”

“…The G+C content of strain DR‐f4 T was 42.6 mol%. Baik et al (2010) reported that the G+C content of the genus Mucilaginibacter is between 42.4 and 47 mol%.…”