Mucin binding mitogenic lectin from freshwater Indian gastropod Belamyia bengalensis: purification and molecular characterization

Banerjee, Sourav; Chaki, Seiichi; Bhowal, Jayati; Chatterjee, B. P.

doi:10.1016/j.abb.2003.09.040

Cited by 25 publications

(12 citation statements)

References 34 publications

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“…The change probably occurs on the carbohydrates of the protein. Previous studies with lectins from Belamyia bengalensis [30] and Macrophomina phaseolina [31] have shown that treatment with sodium metaperiodate causes a significant reduction in hemagglutinating activity. SFL was resistant to treatment with urea, and it maintained HA at (256 HA/mL).…”

Section: Resultsmentioning

confidence: 98%

Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L.

Braga

Lacerda

Medeiros

et al. 2014

Appl Biochem Biotechnol

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The aim of this study was to isolate, characterize, and verify possible antibacterial and hemolytic activity for a lectin found in the seeds of Sterculia foetida L. Purification of the lectin from S. foetida (SFL) was realized with ion exchange chromatography DEAE-Sephacel coupled to HPLC. The purity and the molecular weight was determined by SDS-PAGE. The isolated SFL was characterized as to its glycoprotein nature, and sugar specificity, as well as resistance to pH, temperature, denaturing agents, reduction, oxidation, and chelation. A microdilution method was used to determine antibacterial activity, and hemolytic activity was observed in human erythrocytes. The SFL has a molecular weight of 17 kDa, and a carbohydrate content of 53 μg/mL, specific for arabinose and xylose, and is resistant to treatment with urea, sensitive to treatment with sodium metaperiodate and β-mercaptoethanol, and in the presence of EDTA lost its hemagglutinating activity (HA). However, in the presence of divalent cations (Ca(2 +) and Mn(2 +)) the HA was increased. The SFL remained active even after incubation at 80 °C, and, within pH values of between 5 and 11. The SFL inhibited the bacterial growth of all the tested strains and caused little hemolysis in human erythrocytes when compared to the positive control Triton X-100.

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Section: Resultsmentioning

confidence: 98%

Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L.

Braga

Lacerda

Medeiros

et al. 2014

Appl Biochem Biotechnol

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“…Recently, the importance of protein-protein interaction has been pointed out in certain oligomeric lectins since differences among their quaternary organizations appear to be directly related to those among their functions [26]. Lectin binding sites have been deeply characterized by many workers to understand carbohydrate interaction of these versatile proteins [27–29]. …”

Section: Resultsmentioning

confidence: 99%

Ferromagnetic Levan Composite: An Affinity Matrix to Purify Lectin

Angeli

Paz

Maciel

et al. 2009

BioMed Research International

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A simple and inexpensive procedure used magnetite and levan to synthesize a composite recovered by a magnetic field. Lectins from Canavalia ensiformis (Con A) and Cratylia mollis (Cramoll 1 and Cramoll 1, 4) did bind specifically to composite. The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution. Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column.

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“…The hemagglutination titre was expressed as the reciprocal of the highest dilution of the protein solution showing visible agglutination. Thereafter, the specific hemagglutination activity was calculated as the hemagglutination titer per mg protein per ml and expressed as units [25]. ConA served as a positive control, while PBS was used as a negative control.…”

Section: Hemagglutination Assaymentioning

confidence: 99%

Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells

Jeurink

Lull

Savelkoul

et al. 2008

International Immunopharmacology

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Mucin binding mitogenic lectin from freshwater Indian gastropod Belamyia bengalensis: purification and molecular characterization

Cited by 25 publications

References 34 publications

Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L.

Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L.

Ferromagnetic Levan Composite: An Affinity Matrix to Purify Lectin

Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells

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