Mucopolysaccharide secretion from Drosophila salivary gland cells as a consequence of hormone induced gene activity

Poels, C.L.M.

doi:10.1016/0045-6039(72)90030-9

Cited by 76 publications

(13 citation statements)

References 18 publications

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“…The glue secretory granules differ morphologically from the granules of all other members of the melanogaster group studied so far (Thomopoulos and Kastritsis 1979;Thomopoulos 1987b) as well as from the secretory granules of Drosophila pseudoobscura (Harrod and Kastritsis 1972b), D. hydei (Berendes 1965;Poels 1972), D. virilis (Kress 1974), and D. lebanonensis (Eeken 1977). In D. virilis, the salivary gland secretory granules are homogeneous, having a paracrystalline structure (Kress 1974), and two main secretory proteins have been detected (Kress 1982).…”

Section: Discussionmentioning

confidence: 96%

An ultrastructural and histochemical developmental study of Drosophila auraria salivary gland cells during the third-instar period

Thomopoulos¹,

Neophytou²,

Kastritsis³

1989

Can. J. Zool.

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Early in the third-instar stage of Drosophila auraria, the salivary gland cells produce small secretory granules of low electron density which empty their flocculent contents into the lumen of the gland. At that stage, the Golgi complexes consist of vesiculated, round cisternae which, as middle third instar is approached, change to their classical appearance. Cytoplasmic protrusions, intramitochondrial granules, and close contacts of mitochondria with lipid droplets are observed during the early developmental stages. At about the middle of the third instar the glue secretory granules start being produced. These secretory granules contain a granular material (where small amounts of vicinal glycol and sulfated groups of complex carbohydrates have been detected) of medium electron density. The glue secretory granules can be classified according to their electron density into two populations. Before exocytosis large amounts of glycogen particles are observed, providing some of the energy needed during the final step of exocytosis. Two different types of ribonucleoprotein particles in puffing sites of the chromosomes are observed. Of these, the larger ones consist of a core surrounded by smaller particles; we suspect that they may be produced in the Balbiani rings observed in the polytene chromosomes of this species. Nuclear blebs and oval bodies are common, particularly during the late developmental stages. The functional significance of these findings during development is discussed.

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Section: Discussionmentioning

confidence: 96%

An ultrastructural and histochemical developmental study of Drosophila auraria salivary gland cells during the third-instar period

Thomopoulos¹,

Neophytou²,

Kastritsis³

1989

Can. J. Zool.

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“…In addition, Poels (1972) and later Boyd and Ashburuer (1977) showed that cultured glands are able to release accunmlated secretion into the lumen during early stages (within 4-8 h) of ecdysone-induced puffing. However, no in vitro studies have previously attempted to correlate the puffing pattern in cultured glands with the complex ultrastructural changes of the cytoplasm during the larval/prepupal cycle and thus prove the physiological significance of the observed puffing pattern.…”

mentioning

confidence: 96%

Ultrastructural changes of Drosophila larval and prepupal salivary glands cultured in vitro with ecdysone

Farkas

Sutáková

1998

In Vitro Cell.Dev.Biol.-Animal

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Alterations in the ultrastructure of in vitro cultured larval salivary glands of Drosophila melanogaster in response to the steroid hormone ecdysone were studied in relation to complex changes in puffing patterns. We found that the changes in the fine structure of cultured glands reflected progression of the puffing pattern, and they paralleled those seen in vivo. We observed that glue secretion by exocytosis, the main function of salivary glands, took place between puff stage 5 (PS5) and PS7. Glue could not be expectorated under culture conditions but was slowly released from the lumen through a duct into the medium. After the cultured glands reached PS13/PS14, further progress of puffing and fine structural alterations required that the ecdysteroid titer be transiently extremely low or absent. Under in vitro conditions we did not observe the putative new secretory program(s) described for glands in vivo after PS12. However, ultrastructural changes which unambiguously indicated that an autohistolytic process had begun in vitro started to appear after PS17. Many salivary gland cells developed numerous features of progressive self-degradation between PS18 and PS21. Actual degradation of salivary glands in vivo seemed to be rapid, but in vitro degradation was never completed, probably due to a lack of exogenous factors from the hemolymph. Manipulations of ecdysone titer in vitro in the culture medium, known during the larval puffing cycle to cause premature induction of developmentally specific puffing patterns, did not affect the normal development of ultrastructural features of the cytoplasm and nucleus.

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“…The salivary glands were fixed by means of a staple placed over the duct. The control medium used was a modified Shields and Sang medium described by Poels (1972). The incubation media used during the experiments were: (a) Control medium; (b) Control medium+2 x 10 3M adenosine 31:5'-cyclic-monophosphoric acid (Sigma Chemical Company); (c) Control medium + 10-3 M N 6, 02 t-dibutyryl adenosine 3':5'-cyclic-monophosphoric acid (monosodium salt) (Sigma Chemical Company); (d) Control medium + 100 ~tg ecdysterone/ml; (e) Control medium + 5 • 10 3M 1,3-dimethylxanthine (theophylline) (Sigma Chemical Company).…”

Section: Culture Methods and Mediamentioning

confidence: 99%

Cell communication: A cyclic-AMP mediated phenomenon

1974

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Mucopolysaccharide secretion from Drosophila salivary gland cells as a consequence of hormone induced gene activity

Cited by 76 publications

References 18 publications

An ultrastructural and histochemical developmental study of Drosophila auraria salivary gland cells during the third-instar period

An ultrastructural and histochemical developmental study of Drosophila auraria salivary gland cells during the third-instar period

Ultrastructural changes of Drosophila larval and prepupal salivary glands cultured in vitro with ecdysone

Cell communication: A cyclic-AMP mediated phenomenon

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