Mucosal immunization of sheep with a Maedi-Visna virus (MVV) env DNA vaccine protects against early MVV productive infection

González, Belén; Reina, Ramsés; García, Iker; Andres, Sara N.; Glaria, Idoia; Alzueta, María; Mora, María I.; Jugo, Begoña M.; Arrieta-Aguirre, Inés; Lastra, José Manuel Pérez de la; Rodrı́guez, Dolores; Rodríguez, Juan; Esteban, Mariano; Grilló, María Jesús; Blacklaws, Barbara; Harkiss, G. D.; Chebloune, Yahia; Luján, Lluís; Andrés, D. de; Amorena, B.

doi:10.1016/j.vaccine.2005.03.032

Cited by 34 publications

(39 citation statements)

References 66 publications

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“…However, recent vaccination studies in goats and sheep have shown that env immunization can induce lower virus loads or disease [12] and [19]. Significant differences between these studies and the present work exist.…”

Section: Discussioncontrasting

confidence: 67%

“…This feature would be important for pathogens such as VMV and CAEV that use the mucosal route. In a recent study by Gonzalez et al [19], particle--mediated bombardment of mucosal tissues was used to immunize sheep with the VMV gene (env) encoding the two envelope proteins. The results showed reduced virus load over a period of 18 months before the challenge virus re--appeared.…”

Section: Introductionmentioning

confidence: 99%

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Systemic DNA immunization against ovine lentivirus using particle-mediated epidermal delivery and modified vaccinia Ankara encoding the gag and/or env genes

Niesalla

Andrés

Barbezange

et al. 2009

Vaccine

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To determine whether systemic immunization with plasmid DNA and virus vector against visna/maedi virus (VMV) would induce protective immune responses, sheep were immunized with VMV gag and/or env sequences using particle--mediated epidermal bombardment and injection of recombinant modified vaccinia Ankara. The results showed that immunization induced both humoral and cell--mediated responses prior to and after virus challenge. The vaccination protocol did not prevent infection, but immunization with the gag gene or a combination of gag and env genes resulted in significantly reduced provirus loads in blood and mediastinal lymph node, respectively. Provirus loads in lung and draining lymph node were unaffected, but p25 expression was undetectable in lungs of animals immunized with a combination of gag and env genes. Analysis of target tissues for lesions at post--mortem showed that immunization with the env gene caused a significant increase in lesion score, while the gag gene or a combination of gag and env genes had no effect. Inclusion of the ovine interferon--γ gene in the initial priming mixture had minimal effect on immune responses, provirus load, or lesion development, although it resulted in a decreased p25 expression in the lung. The results thus show that systemic immunization with gag or a combination of gag and env genes reduces provirus load in blood and lymphoid tissue, respectively whereas env immunization has no effect on provirus load but increased lesion development.

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Section: Discussioncontrasting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Systemic DNA immunization against ovine lentivirus using particle-mediated epidermal delivery and modified vaccinia Ankara encoding the gag and/or env genes

Niesalla

Andrés

Barbezange

et al. 2009

Vaccine

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“…SRLV infections occur almost worldwide and cause relevant economic losses. Therapy and effective vaccine are not yet available (Gonzalez et al, 2005, Petursson et al, 2005, Torsteinsdottir et al, 2007, Niesalla et al, 2009and Reina et al, 2008, therefore eradication and prevention of the infection largely depend on early, efficient and correct identification of infected animals (de Andrés et al, 2005). This is routinely done by serological analysis, the ELISA assay being the most sensitive and suitable technique both for large--scale screening and individual examination.…”

Section: Introductionmentioning

confidence: 99%

Seroconversion against SU5 derived synthetic peptides in sheep experimentally infected with different SRLV genotypes

Carrozza¹,

Mazzei²,

Lacerenza³

et al. 2009

Veterinary Microbiology

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Synthetic peptides were generated, corresponding to SU5 domain of envelope glycoprotein of Italian SRLV isolates It--561 and It--Pi1, belonging respectively to MVV--and CAEV--like genotypes. The peptides, encompassing an N--terminal variable and a C--terminal conserved antibody--binding site, were used in an ELISA assay to analyse the sera of two groups of sheep experimentally infected with these isolates. The kinetics and specificity of the humoral response to the homologous and heterologous antigen and the affinity maturation of the sera were evaluated. Seroconversion occurred between week 3 and 8. The response to SU5 antigen was mostly type--specific. The few broadly reacting sera may reflect the production of antibodies directed to the SU5 constant antibody--binding site. All sera underwent with time avidity maturation, resulting in the appearance of high affinity antibodies. This study suggests constant monitoring of the circulating viral variants to develop a panel of diagnostic peptides representative of local genotypes.

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“…Cells were infected at an apparent multiplicity of infection (MOI) of 0.2, and 16 h later MDTF total DNA was purified using a QIAamp DNA minikit (Qiagen). TaqMan quantitative PCR (qPCR) was used to measure viral DNA synthesis using a plasmid standard curve as described previously (9). Strain Ev1 entered and was reverse transcribed in the MDTF cells.…”

mentioning

confidence: 99%

Ovine TRIM5α Can Restrict Visna/Maedi Virus

Jáuregui

Crespo

Glaria

et al. 2012

J Virol

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The restrictive properties of tripartite motif-containing 5 alpha (TRIM5␣) from small ruminant species have not been explored. Here, we identify highly similar TRIM5␣ sequences in sheep and goats. Cells transduced with ovine TRIM5␣ effectively restricted the lentivirus visna/maedi virus DNA synthesis. Proteasome inhibition in cells transduced with ovine TRIM5␣ restored restricted viral DNA synthesis, suggesting a conserved mechanism of restriction. Identification of TRIM5␣ active molecular species may open new prophylactic strategies against lentiviral infections.S mall ruminant lentiviruses (SRLV), including visna/maedi virus (VMV) and caprine encephalitis virus (CAEV), are widespread in sheep and goats, causing a slow progressive disease. Since neither treatment nor efficient vaccines are available, infection is commonly controlled by early diagnosis and culling (23). Recently, the study of host cell restriction factors interfering with the retroviral life cycle, such as the tripartite motif-containing 5 (TRIM5) protein, has gained interest (10, 37). TRIM5 family members bear a RING-B-box-coiled-coil structure consisting of an N-terminal RING domain (with E3 ubiquitin ligase activity), a B-box domain, and a coiled-coil domain (19). The TRIM5␣ isoform, which is active against retroviruses, contains a C-terminal PRYSPRY domain that binds retroviral capsid CA (12,20,35). This interaction, involving amino acid 332 of TRIM5␣ in humans (15) and 334 in monkeys, may explain the high relative rates of nonsynonymous changes of the primate TRIM5␣ gene (13). TRIM5␣ has been described in primates and several mammals (3, 6, 30, 33, 41) but not in sheep or goats, both of which are infected by SRLV, their own lentivirus. This study aimed to identify and characterize the ovine and caprine TRIM5␣ proteins and explore the possible restrictive role of ovine TRIM5␣ on VMV infection.First, we cloned and sequenced ovine and caprine TRIM5␣ cDNA sequences. For this, total RNA from ovine skin fibroblasts (SF), bronchoalveolar lavage (BAL) fluid, or lung tissue obtained from domestic sheep of the Assaf (n ϭ 3), Churra (n ϭ 2), and Rasa Aragonesa (n ϭ 4) breeds was purified using TRIzol (Invitrogen) passed through RNeasy minikit columns (Qiagen), before being reverse transcribed with SuperScript II (Invitrogen) using an oligo(dT) primer according to the manufacturer's instructions. To clone the caprine counterpart, cDNA from peripheral blood mononuclear cells (PBMC) from goats of the Roccaverano (n ϭ 1) and Murciano-Granadina (n ϭ 2) breeds was used. These cDNAs were employed as the PCR template using Phusion high-fidelity DNA polymerase (Finnzymes) with the forward primer TrimEXNFw (5=-TGCA CCTCGAGATGGCTTCAGGAATCCTG-3=, XhoI site underlined) and the reverse primer PJ2 (5=-GATCCGGGCCCTCAAC AGCTTGGTGAGC-3=, ApaI site underlined) following standard thermal profiles. Amplified products were cloned into the TOPO Blunt vector (Invitrogen) as a shuttle/sequencing vector, yielding a total of 12 ovine and 5 caprine independent sequences. Four ov...

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Mucosal immunization of sheep with a Maedi-Visna virus (MVV) env DNA vaccine protects against early MVV productive infection

Cited by 34 publications

References 66 publications

Systemic DNA immunization against ovine lentivirus using particle-mediated epidermal delivery and modified vaccinia Ankara encoding the gag and/or env genes

Systemic DNA immunization against ovine lentivirus using particle-mediated epidermal delivery and modified vaccinia Ankara encoding the gag and/or env genes

Seroconversion against SU5 derived synthetic peptides in sheep experimentally infected with different SRLV genotypes

Ovine TRIM5α Can Restrict Visna/Maedi Virus

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