Mucosal immunization with a ricin toxin B subunit–rotavirus NSP4 fusion protein stimulates a Th1 lymphocyte response

Choi, Nak-Won; Estes, Mary K.; Langridge, William H. R.

doi:10.1016/j.jbiotec.2005.07.024

Cited by 27 publications

(19 citation statements)

References 38 publications

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“…Further applications of RIPs include the use of B chains in the preparation of conjugates. The intracellular transport of molecules has been obtained with an entirely different, almost reverse approach, with ricin B chain as a carrier of antigens [202,203]. Furthermore, a fusion protein combining the endotoxin of Bacillus thuringiensis with the ricin B chain was prepared, and transgenic rice and maize plants expressing the fusion protein were more toxic to insects than plants containing the toxin gene alone [204].…”

Section: Removal Of Cells From Cultures [175]mentioning

confidence: 99%

Ribosome-inactivating proteins: progress and problems

Stirpe

Battelli

2006

Cell. Mol. Life Sci.

321

225

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Ribosome-inactivating proteins (RIPs), mostly from plants, are enzymes which depurinate rRNA, thus inhibiting protein synthesis. They also depurinate other polynucleotide substrates. The biological activity of RIPs is not completely clarified, and sometimes independent of the inhibition of protein synthesis. There are differences in the cytotoxicity of RIPs and, consequently, in their toxicity to animals. Some RIPs are potent toxins, the best known being ricin, a potential biological weapon. New toxins have recently been identified. RIPs cause apoptotic and necrotic lesions, and induce production of cytokines causing inflammation. RIPs are potentially useful in agriculture and medicine because (i) they have antiviral activity and (ii) they are used for the preparation of conjugates with antibodies ('immunotoxins') or other carriers, rendering them specifically toxic to the cell target of the carrier, which may be helpful in therapy. The distribution, mechanism of action and role in nature of RIPs are not completely understood, and we can expect several future developments in their practical application.

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Section: Removal Of Cells From Cultures [175]mentioning

confidence: 99%

Ribosome-inactivating proteins: progress and problems

Stirpe

Battelli

2006

Cell. Mol. Life Sci.

321

225

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“…However, other studies have demonstrated that VP6-induced T cell responses and cytokine secretion rather than antibody induction were critical for rotavirus defense in B cell-deficient mice (Choi et al, 1999; Franco and Greenberg, 1999). Studies investigating NSP4 reported similar results demonstrating the involvement of antibody responses, T cells and cytokines (Enouf et al, 2001; Ray et al, 2003; Vizzi et al, 2005; Choi et al, 2006). In this study, oral administration of a maize seed-based vaccine expressing VP6 and LTB-NSP4 fusion protein stimulated both systemic and mucosal immunity as indicated by the induction of serum IgG and mucosal IgA ( Figure 5 ).…”

Section: Discussionmentioning

confidence: 63%

Oral Administration of a Seed-based Bivalent Rotavirus Vaccine Containing VP6 and NSP4 Induces Specific Immune Responses in Mice

Feng

Song

et al. 2017

Front. Plant Sci.

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Rotavirus is the leading cause of severe diarrheal disease among newborns. Plant-based rotavirus vaccines have been developed in recent years and have been proven to be effective in animal models. In the present study, we report a bivalent vaccine candidate expressing rotavirus subunits VP6 and NSP4 fused with the adjuvant subunit B of E. coli heat-labile enterotoxin (LTB) in maize seeds. The RT-PCR and Western blot results showed that VP6 and LTB-NSP4 antigens were expressed and accumulated in maize seeds. The expression levels were as high as 0.35 and 0.20% of the total soluble protein for VP6 and LTB-NSP4, respectively. Oral administration of transgenic maize seeds successfully stimulated systemic and mucosal responses, with high titers of serum IgG and mucosal IgA antibodies, even after long-term storage. This study is the first to use maize seeds as efficient generators for the development of a bivalent vaccine against rotavirus.

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“…However, these fusion proteins are found in inclusion bodies and therefore have to be refolded after denaturation with urea. 17,18 In this study, not only immunization but also protection from tumor challenge was tested in C57BL/6 mice by using the tumor cell line TC-1, which expresses HPV-16 E7. In the immunization model, we demonstrated that the injection of E7-RTB in soluble form protected the mice from tumor challenge completely, even in the absence of adjuvant.…”

Section: Discussionmentioning

confidence: 99%