Mucosal immunization with HIV-1 gag DNA on cationic microparticles prolongs gene expression and enhances local and systemic immunity

Singh, Manmohan; Vajdy, Michael; Gardner, Jason P.; Briones, Maylene; O’Hagan, Derek T.

doi:10.1016/s0264-410x(01)00321-8

Cited by 76 publications

(43 citation statements)

References 53 publications

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“…before a lethal challenge with influenza virus (18). Limitations that may arise after mucosal DNA vaccination may be overcome, potentiating its effectiveness by adsorption of DNA into positively charged polylactide-coglycolide microparticles (19), or codelivering the DNA that encodes for the specific Ag with DNA vectors encoding cytokines (20). Also, coadministration of DNA vectors with mucosal adjuvants have been proved to be as an efficient strategy, giving protection against equine influenza in a pony model (21).…”

Section: Induction Of Hiv Immunity In the Genitalmentioning

confidence: 99%

Induction of HIV Immunity in the Genital Tract After Intranasal Delivery of a MVA Vector: Enhanced Immunogenicity After DNA Prime-Modified Vaccinia Virus Ankara Boost Immunization Schedule

Gherardi

Pérez-Jiménez

Nájera

et al. 2004

The Journal of Immunology

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Vaccines intended to prevent mucosal transmission of HIV should be able to induce multiple immune effectors in the host including Abs and cell-mediated immune responses at mucosal sites. The aim of this study was to characterize and to enhance the immunogenicity of a recombinant modified vaccinia virus Ankara (MVA) expressing HIV-1 Env IIIB Ag (MVAenv) inoculated in BALB/c mice by mucosal routes. Intravaginal inoculation of MVAenv was not immunogenic, whereas intranasally it induced a significant immune response to the HIV Ag. Intranasal codelivery of MVAenv plus cholera toxin (CT) significantly enhanced the cellular and humoral immune response against Env in the spleen and genitorectal draining lymph nodes, respectively. Heterologous DNAenv prime-MVAenv boost by intranasal immunization, together with CT, produced a cellular immune response in the spleen 10-fold superior to that in the absence of CT. A key finding of these studies was that both MVAenv/MVAenv and DNAenv/MVAenv schemes, plus CT, induced a specific mucosal CD8+ T cell response in genital tissue and draining lymph nodes. In addition, both immunizations also generated systemic Abs, and more importantly, mucosal IgA and IgG Abs in vaginal washings. Specific secretion of β-chemokines was also generated by both immunizations, with a stronger response in mice immunized by the DNA-CT/MVA-CT regimen. Our findings are of relevance in the area of vaccine development and support the optimization of protocols of immunization based on MVA as vaccine vectors to induce mucosal immune responses against HIV.

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Section: Induction Of Hiv Immunity In the Genitalmentioning

confidence: 99%

Induction of HIV Immunity in the Genital Tract After Intranasal Delivery of a MVA Vector: Enhanced Immunogenicity After DNA Prime-Modified Vaccinia Virus Ankara Boost Immunization Schedule

Gherardi

Pérez-Jiménez

Nájera

et al. 2004

The Journal of Immunology

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“…For HIV-1, in particular, a number of different mucosal vaccine strategies have been studied, including bacterial vectors (17,28,37,50), proteins (2,52), live-attenuated vectors (4,26), inactivated HIV-1 (1,20), VLPs (61), and naked DNA (40,55).…”

mentioning

confidence: 99%

Induction of Systemic and Mucosal Cross-Clade Neutralizing Antibodies in BALB/c Mice Immunized with Human Immunodeficiency Virus Type 1 Clade A Virus-Like Particles Administered by Different Routes of Inoculation

Buonaguro

Visciano

Tornesello

et al. 2005

J Virol

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“…It is well known that many candidate vaccines are ineffective such as HIV-1 vaccines [25] and should be improved with new approaches such as formulation in new candidate adjuvants [26]. So that, use of vaccine adjuvanted with some natural compounds may be useful for improvement of vaccine efficacy [27].…”

Section: Discussionmentioning

confidence: 99%

Adjuvant Effect of Royal Jelly on HIV-1 Multi-Epitope Vaccine Candidate: Induction of Th1 Cytokine Pattern

Mahdavi¹,

Cheragh²,

Bagheri³

et al. 2017

MOJI

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Mucosal immunization with HIV-1 gag DNA on cationic microparticles prolongs gene expression and enhances local and systemic immunity

Cited by 76 publications

References 53 publications

Induction of HIV Immunity in the Genital Tract After Intranasal Delivery of a MVA Vector: Enhanced Immunogenicity After DNA Prime-Modified Vaccinia Virus Ankara Boost Immunization Schedule

Induction of HIV Immunity in the Genital Tract After Intranasal Delivery of a MVA Vector: Enhanced Immunogenicity After DNA Prime-Modified Vaccinia Virus Ankara Boost Immunization Schedule

Induction of Systemic and Mucosal Cross-Clade Neutralizing Antibodies in BALB/c Mice Immunized with Human Immunodeficiency Virus Type 1 Clade A Virus-Like Particles Administered by Different Routes of Inoculation

Adjuvant Effect of Royal Jelly on HIV-1 Multi-Epitope Vaccine Candidate: Induction of Th1 Cytokine Pattern

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