Mucosal interleukin‐8 expression as a predictor of subsequent relapse in ulcerative colitis patients with Mayo endoscopic subscore 0

Uchiyama, Kazuhiko; Takagi, Tomohisa; Mizushima, Katsura; Asaeda, Kohei; Kajiwara, Mariko; Kashiwagi, Saori; Toyokawa, Yuki; Hotta, Yuma; Tanaka, Makoto; Inoue, Ken; Dohi, Osamu; Okayama, Tetsuya; Yoshida, Naohisa; Katada, Kazuhiro; Kamada, Kazuhiro; Ishikawa, Takeshi; Yasuda, Hiroaki; Konishi, Hideyuki; Kishimoto, Mitsuo; Naito, Yuji; Itoh, Yoshito

doi:10.1111/jgh.15813

Cited by 8 publications

(5 citation statements)

References 47 publications

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“…Studies have shown that IL-4 in UC could induce monocytes and eosinophils to participate in inflammatory response and stimulate mast cell proliferation, thus promoting inflammatory response and aggravating the degree of UC. 78 , 79 The level of peripheral 5-HT, a pro-inflammatory factor, which could regulate cellular immune function, promote emission of IFN-γ and other inflammatory factors, 80 and also aggravate inflammatory response of UC, was positively correlated with the severity of UC, 80 , 81 Taken together, DSS could increase the abundance of Clostridium_sensu_stricto_1 relative pro-inflammatory factors, promotes the release of IL-4, 5-HT, TNF-α and IFN-γ, which could promote intestinal inflammation, leading to ulcerative colitis.…”

Section: Discussionmentioning

confidence: 99%

Dynamic Changes in Gut Microbiome of Ulcerative Colitis: Initial Study from Animal Model

Gu¹,

Zhang²,

Han³

et al. 2022

JIR

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Background An animal model of DSS-induced UC has been widely used in basic research, and the dysbiosis of gut microbiome is one of the important pathogenetic mechanisms of DSS-induced UC, but its dynamic changes and correlation with inflammatory factors are not clear yet. Methods Clinical signs and tissue damage degree of C57BL/6 ulcerative colitis mice model induced by different concentrations of DSS were compared with that of normal mice, and finally the optimal concentration of DSS was determined. Then we analyzed the sequencing results of gut microbiome and inflammatory factors to determine the dynamic patterns of gut microbiome and their correlation with the inflammatory factors. Results DSS at 2.5% and 3.0% concentration could cause intestinal injury and induce colitis. However, 3.0% DSS resulted in higher mortality. In addition, there were dynamic changes of gut microbiome in DSS-induced UC model: the relative abundance of intestinal flora increased first and then decreased in Bacteroides , Parabacteroides , Romboutsia , Clostridium_sensu_stricto_1, Lachnospiraceae_NK4A136_group, norank_f_norank_o_Clostridia_UCG-014, Parasutterella , and decreased first and then increased in Lactobacillus , Muribaculum , norank_f_Muribaculaceae, in addition, Bifidobacterium , Coriobacteriaceae_UCG-002 and Enterorhabdus did not change in the first 14 days but increased significantly on day 21. Moreover, inflammatory cytokines were closely associated with the imbalance of the intestinal microbiota in mice with UC: most pathogenic bacteria in the intestinal tract of the UC animal model were positively correlated with pro-inflammatory factors and negatively correlated with anti-inflammatory factors, while beneficial bacteria were the opposite. Conclusion Intestinal microecology plays an important role in DSS-induced UC model, and the relative abundance of gut microbiome changes dynamically in the occurrence and development of ulcerative colitis.

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Section: Discussionmentioning

confidence: 99%

Dynamic Changes in Gut Microbiome of Ulcerative Colitis: Initial Study from Animal Model

Gu¹,

Zhang²,

Han³

et al. 2022

JIR

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“…Expression of some pro-inflammatory cytokines and chemokines correlates to endoscopic disease activity. ( 28 , 29 ) We analyzed mRNA expression of cytokines and chemokines based on MES. We could not perform statistical analyses since three UC flare-up patients were judged as MES 2 ( n = 2) and MES 3 ( n = 1).…”

Section: Resultsmentioning

confidence: 99%

“…( 33 , 34 ) UC relapse was defined as aggravation of UC-associated symptoms and UC aggravation based on endoscopic findings. ( 28 , 29 ) Endoscopic and histological disease activities were assessed by MES and Nancy histological index, respectively. The clinical characteristics of the patients with active UC not associated with COVID-19 vaccination are summarized in Table 2 .…”

Section: Methodsmentioning

confidence: 99%

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Cytokine and chemokine profiles in ulcerative colitis relapse after coronavirus disease 2019 vaccination

Masuta,

Minaga,

Otsuka

et al. 2024

J. Clin. Biochem. Nutr.

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Coronavirus disease 2019 (COVID-19) vaccines are highly effective; however, vaccine-related adverse events, including autoimmunity, have been reported. Case reports describing relapse or new-onset of ulcerative colitis (UC) after COVID-19 mRNA vaccination are available. However, the molecular mechanisms underlying the development of colonic inflammation associated with COVID-19 mRNA vaccination are poorly understood. Furthermore, it is unclear whether the relapse of UC after COVID-19 vaccination is driven by unique cytokine responses that differ from those of UC not associated with vaccination. mRNAs derived from COVID-19 vaccines are potent inducers of type I IFN response. We encountered three cases of UC relapse after COVID-19 vaccination. mRNA expressions of IFN-α, IFN-β, IL-1β, and IL-12/23p40 showed higher tendency in the colonic mucosa of patients with UC associated with vaccination compared with those not associated with vaccination. In contrast, the expressions of C-X-C motif chemokine ligand 9 (CXCL9) and CXCL10 were comparable. Immunofluorescence analyses also showed higher expression of IFN-α in the colonic mucosa of patients with UC associated with COVID-19 vaccination than in those not associated with vaccination. Taken together, these data suggest that the colonic mucosa of patients with UC who relapsed after COVID-19 vaccination was characterized by enhanced type I IFN responses.

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“…ILs are important cytokines involved in a variety of inflammatory pathways, and there have been many studies on their roles in the development, pathogenesis, and treatment mechanism of IBD ( 9 ). For instance, a prospective observational study examined the expression of mucosal cytokines in 55 UC patients and detected the expression level of 10 types of ILs after their recurrence, and confirmed that IL-8 is the most potent predictor of UC recurrence ( 10 ). Meanwhile, clinical research evidence also supports the role of various chemokines in the pathogenesis of IBD.…”

Section: Introductionmentioning

confidence: 92%

Association between inflammatory bowel disease and interleukins, chemokines: a two-sample bidirectional mendelian randomization study

et al. 2023

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BackgroundMendelian randomization (MR) was used to evaluate the bidirectional causal relationship between inflammatory bowel disease (IBD) and interleukins (ILs), chemokines.MethodsGenetic instruments and summary data of five ILs and six chemokines were obtained from a genome-wide association study database, and instrumental variables related to IBD were obtained from the FinnGen Consortium. Inverse variance weighting (IVW) was used as the main MR analysis method, and several other MR methods including MR-Egger and weighted median were used to confirm the reliability of the results. Sensitivity analyses such as heterogeneity and pleiotropy were also performed.ResultsThe IVW method provided evidence to support that genetically predicted IL-16, IL-18, and CXCL10 significantly positively correlated with IBD, while IL-12p70 and CCL23 significantly negatively correlated with IBD. IL-16 and IL-18 had a suggestive association with an increased risk of ulcerative colitis (UC), and CXCL10 had a suggestive association with an increased risk of Crohn’s disease (CD). However, there was no evidence to support that IBD and two main subtypes (UC and CD) are associated with changes in the levels of ILs and chemokines. The results of the sensitivity analyses were robust and no evidence of heterogeneity and horizontal pleiotropy was observed.ConclusionsThe present study showed that some ILs and chemokines affect IBD, but IBD and its main subtypes (UC and CD) have no effect on the level changes of ILs and chemokines.

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Mucosal interleukin‐8 expression as a predictor of subsequent relapse in ulcerative colitis patients with Mayo endoscopic subscore 0

Abstract: This study was partly supported by these funds. Y. N. is an associate editor of "Digestion." Neither the funding agency nor any outside organization participated in the study design or had any competing interests. These companies approved the final version of the manuscript.

Cited by 8 publications

References 47 publications

Dynamic Changes in Gut Microbiome of Ulcerative Colitis: Initial Study from Animal Model

Dynamic Changes in Gut Microbiome of Ulcerative Colitis: Initial Study from Animal Model

Cytokine and chemokine profiles in ulcerative colitis relapse after coronavirus disease 2019 vaccination

Association between inflammatory bowel disease and interleukins, chemokines: a two-sample bidirectional mendelian randomization study

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