Mucuna pruriens (L.) DC chemo sensitize human breast cancer cells via downregulation of prolactin-mediated JAK2/STAT5A signaling

Sinha, Sonam; Sharma, Sonal; Vora, Jaykant; Shah, Heta; Srivastava, Anshu; Shrivastava, Neeta

doi:10.1016/j.jep.2018.02.006

Cited by 26 publications

(13 citation statements)

References 28 publications

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“…For pharmacological properties, M. pruriens seed extract has been shown to have neuroprotective, anti-oxidative, anti-inflammatory, anti-hypertensive, anti-diabetic, anti-venom, and anti-cancer effects. 14 , 15 , 16 Based on phytochemical profiles, the L-3,4 dihydroxyphenyl alanine ( l -DOPA) is demonstrated to be a major active substance found in the seeds of M. pruriens with high antioxidant capacity. 17 , 18 , 19 Moreover, M. pruriens seed has been proven to improve semen quality and antioxidant enzymes in seminal plasma of infertile men.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of Thai Mucuna pruriens (L.) DC. var. pruriens seeds on sexual behaviors and essential reproductive markers in chronic unpredictable mild stress mice

Choowong-In

Sattayasai

Boonchoong

et al. 2022

Journal of Traditional and Complementary Medicine

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Section: Introductionmentioning

confidence: 99%

Protective effects of Thai Mucuna pruriens (L.) DC. var. pruriens seeds on sexual behaviors and essential reproductive markers in chronic unpredictable mild stress mice

Choowong-In

Sattayasai

Boonchoong

et al. 2022

Journal of Traditional and Complementary Medicine

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“…L-dopa is used widely to increase dopamine concentrations in the treatment of Parkinson's disease with a dopa decarboxylase inhibitor, such as carbidopa and benserazide, which inhibit aromatic amino acid decarboxylase, thus preventing the conversion of L-dopa into dopamine [49]. L-dopa was recently reported to suppress cellular growth via down-regulation of prolactin-mediated JAK2/ STAT5A in breast cancer cells [50], and carbidopa was reported to be an aryl hydrocarbon receptor agonist and to suppress the growth of pancreatic cancer [51]. However, the role of L-dopa in oral cancer remains unknown.…”

Section: Discussionmentioning

confidence: 99%

SYT12 plays a critical role in oral cancer and may be a novel therapeutic target

Eizuka¹,

Nakashima²,

Oka³

et al. 2019

J. Cancer

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Synaptotagmin12 (SYT12) has been well characterized as the regulator of transmitter release in the nervous system, however the relevance and molecular mechanisms of SYT12 in oral squamous cell carcinoma (OSCC) are not understood. In the current study, we investigated the expression of SYT12 and its molecular biological functions in OSCC by quantitative reverse transcriptase polymerase chain reaction, immunoblot analysis, and immunohistochemistry. SYT12 were up-regulated significantly in OSCC-derived cell lines and primary OSCC tissue compared with the normal counterparts (P<0.05) and the SYT12 expression levels were correlated significantly with clinical indicators, such as the primary tumoral size, lymph node metastasis, and TNM stage (P<0.05). SYT12 knockdown OSCC cells showed depressed cellular proliferation, migration, and invasion with cell cycle arrest at G1 phase. Surprisingly, we found increased calcium/calmodulin-dependent protein kinase 2 (CAMK2) inhibitor 1 (CAMK2N1) and decreased CAMK2-phosphorylation in the knockdown cells. Furthermore, treatment with L-3, 4-dihydroxyphenylalanine (L-dopa), a drug approved for Parkinson's disease, led to down-regulation of SYT12 and similar phenotypes to SYT12 knockdown cells. Taken together, we concluded that SYT12 plays a significant role in OSCC progression via CAMK2N1 and CAMK2, and that L-dopa would be a new drug for OSCC treatment through the SYT12 expression.

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“…PRL binds to its receptor PRLR and forms a complex that can further activate the Janus kinase 2 (JAK2). Subsequently, the activated JAK2 leads to its downstream signal transducer and activator of transcription 5 (STAT5) [ 10 ]. Phosphorylated STAT5 protein forms homodimers or heterodimerizes with other STAT family members, relating to the nucleus translocation and transactivation of the molecular target.…”

Section: Introductionmentioning

confidence: 99%

Bu-Shen-Zhu-Yun decoction induces PRLR deubiquitination and JAK2/STAT5 activation via CSN5 in vitro

Huang

Zhou

Lü

et al. 2021

Aging

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Purpose: To determine the effect of Bu-Shen-Zhu-Yun Decoction (BSZY-D) on the kisspeptin through JAK2/STAT5 signaling pathway in hyperprolactinemia (HPRL) infertility. Method: SD rats were treated with BSZY-D for cerebrospinal fluid (CSF) extraction. GT1-7 cells were subjected to different treatments. The phosphorylation levels of JAK2 and STAT5, and the expressions of PRLR and kisspeptin of GT1-7 cells in different groups were detected by western blot, RT-qPCR and immunofluorescence. The expressions of CSN5 and GATA1 and other molecular features were checked by western blot, RT-PCR, co-immunoprecipitation and renilla luciferase activity. Results: The phosphorylation levels of JAK2 and STAT5, and the expressions of PRLR and kisspeptin in the HPRL group were significantly decreased, and these changes could be reversed after BSZY-D treatment. In addition, the presence of PRLR deubiquitination was detected in the HPRL group, which could be reversed by shRNA-CSN5, suggesting that BSZY-D played a role through targeting CSN5. The binding level of GATA1 and CSN5 promoter in the HPRL group was significantly decreased, but elevated in the HPRL (BSZY-D/CSF) group (P < 0.05). Conclusion: BSZY-D improved the transcription activity of GATA1 and increased the binding of GATA1 and CSN5. BSZY-D was involved in the deubiquitination of PRLR, which contributes to alleviating the symptoms of HPRL infertility.

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Mucuna pruriens (L.) DC chemo sensitize human breast cancer cells via downregulation of prolactin-mediated JAK2/STAT5A signaling

Cited by 26 publications

References 28 publications

Protective effects of Thai Mucuna pruriens (L.) DC. var. pruriens seeds on sexual behaviors and essential reproductive markers in chronic unpredictable mild stress mice

Protective effects of Thai Mucuna pruriens (L.) DC. var. pruriens seeds on sexual behaviors and essential reproductive markers in chronic unpredictable mild stress mice

SYT12 plays a critical role in oral cancer and may be a novel therapeutic target

Bu-Shen-Zhu-Yun decoction induces PRLR deubiquitination and JAK2/STAT5 activation via CSN5 in vitro

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