Mud crab Scylla paramamosain glutamate dehydrogenase: molecular cloning, tissue expression and response to hyposmotic stress

Lu, Jing-Ying; Shu, Miao-An; Xu, Bing-Peng; Liu, Guangxu; Ma, Yupo; Xia-zhen, Guo; Liu, Yu

doi:10.1007/s12562-014-0828-5

Cited by 34 publications

(10 citation statements)

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“…Current research on crustacean Na + / K + -ATPase has been extensively reported [ 10 , 23 ] suggesting that it is a widespread P-type ATPase that plays an important role in maintaining Na + , K + homeostasis [ 11 ]. As all known, free amino acids [ 1 , 25 , 39 , 42 , 43 ] also played an important role in osmoregulation. In addition, several pathways connected with amino acid metabolism were detected, such as for arginine biosynthesis (ko00220) (Additional file 1 : Table S8), alanine, aspartate and glutamate metabolism (ko00250) (Additional file 1 : Table S8), lysine degradation (ko00310) (Additional file 1 : Table S8), valine, leucine and isoleucine degradation (ko00280) (Additional file 1 : Table S8), amino sugar and nucleotide sugar metabolism (ko00520) (Additional file 1 : Table S8), biosynthesis of amino acids (ko01230) (Additional file 1 : Table S8), pyrimidine metabolism (ko00240) (Additional file 1 : Table S8).…”

Section: Resultsmentioning

confidence: 99%

“…To date, reports on the regulation of infiltration of S. paramamosain are still quite limited to cloning and expression of the Na + / K + -ATPase [ 8 ] and cloning and expression of glutamate dehydrogenase (GDH) [ 25 ]. GHD is an important enzyme for the metabolism of glycine, proline, and alanine, which serve as general osmolytes in aquatic animals.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have shown that low salinity influences ion channel activity [ 33 , 37 , 44 , 45 ] and L-type free amino acids [ 1 , 25 , 39 , 42 , 43 ], which are tightly involved with osmoregulation. In particular, the Na + / K + -ATPase, a well-known ion channel, is the main ion transport enzyme of post-larvae in crustaceans.…”

Section: Introductionmentioning

confidence: 99%

“…Chung and Lin [ 8 ] cloned the full-length α-subunit of the Na + / K + -ATPase cDNA, indicating the osmoregulatory role of the channel via both mRNA and protein expression. Likewise, Lu et al [ 25 ] completed the cDNA cloning of glutamate dehydrogenase and its expression, indicating that GDH played an important role in controlling osmoregulation through free amino acids in S. paramamosain .…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic analysis of adaptive mechanisms in response to sudden salinity drop in the mud crab, Scylla paramamosain

et al. 2018

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BackgroundScylla paramamosain (Crustacea: Decapoda: Portunidae: Syclla De Hann) is a commercially important mud crab distributed along the coast of southern China and other Indo-Pacific countries (Lin Z, Hao M, Zhu D, et al, Comp Biochem Physiol B Biochem Mol Biol 208-209:29–37, 2017; Walton ME, Vay LL, Lebata JH, et al, Estuar Coast Shelf Sci 66(3–4):493–500, 2006; Wang Z, Sun B, Zhu F, Fish Shellfish Immunol 67:612–9, 2017). While S. paramamosain is a euryhaline species, a sudden drop in salinity induces a negative impact on growth, molting, and reproduction, and may even cause death. The mechanism of osmotic regulation of marine crustaceans has been recently under investigation. However, the mechanism of adapting to a sudden drop in salinity has not been reported.MethodsIn this study, transcriptomics analysis was conducted on the gills of S. paramamosain to test its adaptive capabilities over 120 h with a sudden drop in salinity from 23 ‰ to 3 ‰.ResultsAt the level of transcription, 135 DEGs (108 up-regulated and 27 down-regulated) annotated by NCBI non-redundant (nr) protein database were screened. GO analysis showed that the catalytic activity category showed the most participating genes in the 24 s-tier GO terms, indicating that intracellular metabolic activities in S. paramamosain were enhanced. Of the 164 mapped KEGG pathways, seven of the top 20 pathways were closely related to regulation of the Na+ / K+ -ATPase. Seven additional amino acid metabolism-related pathways were also found, along with other important signaling pathways.ConclusionIon transport and amino acid metabolism were key factors in regulating the salinity adaptation of S. paramamosain in addition to several important signaling pathways.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4803-x) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptomic analysis of adaptive mechanisms in response to sudden salinity drop in the mud crab, Scylla paramamosain

et al. 2018

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“…5 and Additional file 1 : Table S3). Free amino acids play important roles in osmoregulation [ 1 , 25 , 46 ]. The results of the current study implied that amino acid metabolism in the gills of S. paramamosain was altered to equilibrate changes in exoteric osmotic pressure.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of miRNAs in the gills of the mud crab (Scylla paramamosain) in response to a sudden drop in salinity

et al. 2018

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BackgroundThe mud crab (Scylla paramamosain) is a euryhaline and commercially important species. MiRNAs participate in the regulation of many physiological activities.ResultsThe miRNA transcriptome of the gills of S. paramamosain was used to investigate the expression profiles of miRNAs in response to a sudden drop in salinity. In total, seven known miRNAs and 43 novel miRNAs were identified, with 18 differentially expressed small RNAs. Fourteen thousand nine hundred fifty-one differentially expressed miRNAs target genes were screened by prediction. GO analysis of differentially expressed miRNAs target genes indicated that 578 genes associated with cellular processes, 523 associated with metabolic processes, and 422 associated with single-organism processes were the most strongly affected by a sudden drop in salinity from 23‰ to 3‰. KEGG pathway analysis showed 14 pathways were related to amino acid metabolism, which plays an important role in osmoregulation. Besides, several pathways were associated with starch and sucrose metabolism (ko00500), glycosaminoglycan degradation (ko00531), and galactose metabolism (ko00052).ConclusionsS. paramamosain regulated osmotic pressure and energy balance by regulating target genes to adapt to a sudden changes in salinity. These results provided a basis for further investigations of miRNA-modulating networks underlying the osmoregulation of S. paramamosain.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4981-6) contains supplementary material, which is available to authorized users.

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Nutrition and Functions of Amino Acids in Aquatic Crustaceans

Han

Zheng³

et al. 2021

Advances in Experimental Medicine and Biology

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Mud crab Scylla paramamosain glutamate dehydrogenase: molecular cloning, tissue expression and response to hyposmotic stress

Cited by 34 publications

References 30 publications

Transcriptomic analysis of adaptive mechanisms in response to sudden salinity drop in the mud crab, Scylla paramamosain

Transcriptomic analysis of adaptive mechanisms in response to sudden salinity drop in the mud crab, Scylla paramamosain

Identification and characterization of miRNAs in the gills of the mud crab (Scylla paramamosain) in response to a sudden drop in salinity

Nutrition and Functions of Amino Acids in Aquatic Crustaceans

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