Mueller matrix imaging of pathological slides with plastic coverslips

Abstract

Mueller matrix microscopy is capable of polarization characterization of pathological samples and polarization imaging based digital pathology. In recent years, hospitals are replacing glass coverslips with plastic coverslips for automatic preparations of dry and clean pathological slides with less slide-sticking and air bubbles. However, plastic coverslips are usually birefringent and introduce polarization artifacts in Mueller matrix imaging. In this study, a spatial frequency based calibration method (SFCM) is used to remove such polarization artifacts. The polarization information of the plastic coverslips and the pathological tissues are separated by the spatial frequency analysis, then the Mueller matrix images of pathological tissues are restored by matrix inversions. By cutting two adjacent lung cancer tissue slides, we prepare paired samples of very similar pathological structures but one with a glass coverslip and the other with a plastic coverslip. Comparisons between Mueller matrix images of the paired samples show that SFCM can effectively remove the artifacts due to plastic coverslip.

3D polarization-interference holographic histology for wavelet-based differentiation of the polycrystalline component of biological tissues with different necrotic states. Forensic applications

“…The fundamental results obtained within the framework of MMM of biological tissues have wide diagnostic application in various fields of medicine. 37 The possibility of obtaining quantitative optical indicators to characterize the evolution of gastric tissue from a healthy state through inflammation to cancer has been demonstrated. 14 The criteria for the Mueller-matrix diagnosis of prostate cancer, 38 bowel, 39 and cervix 40 have been determined.…”

Multispectral polarization microscopy of different stages of human oral tissue: A polarization study

“…However, traditional Mueller matrix imaging requires a large number of intensity measurements, specifically 24 measurements, which are time‐consuming and require complex optical setups and their alignment. Although the use of a single‐shot Mueller polarimeter can reduce the number of measurements, the system becomes very costly [34–36]. In the present manuscript, we introduce a novel approach: multispectral Stokes imaging, which only requires six intensity measurements.…”