Mueller-matrix mapping of optically anisotropic fluorophores of molecular biological tissues in the diagnosis of death causes

Ushenko, A. G.; Dubolazov, A. V.; Ушенко, В. А.; Pidkamin, L. Y.; Soltys, I. V.; Zhytaryuk, V. G.; Pavlyukovich, N.

doi:10.1117/12.2237662

Cited by 7 publications

(9 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently, the samples were sent to the laboratory of the Yuriy Fedkovych Institute of Physical, Technical and Computer Sciences, where they were investigated using the CDMA method. The application method is described in these sources (Vanchulyak, 2019;Ushenko, 2016;Bachinskiy, 2017). Results and discussion.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, more and more attention is being paid to optical methods for the study of biological tissues using lasers which, by studying the lightscattering phenomena, make it possible to obtain reliable objective data on the dynamics of pre-and post-mortem changes of the studied tissues of the human body and with greater accuracy to diagnose CD because of hemorrhage into the substance of the brain (Bachinskiy, 2017). At present, preliminary results of the polarization study of birefringence of optically thin specimens of pathologically-altered tissues are known, which were carried out in the laboratory of the Yuriy Fedkovych National University Institute of Physical, Technical Sciences, and Computer Science, which allows predicting the effective use of these methods for further verifications of hemorrhage into HBS (Ushenko, 2016;.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Methods to Determine Complex Degrees of Mutual Anisotropy for the Differentiation of Hemorrhages in the Human Brain Substance Resulting From Traumatic and Non-Traumatic Genesis

Garazdiuk

Bachynskіy

Nechytailo

et al. 2020

PMP

View full text Add to dashboard Cite

An issue that is often debated in forensic traumatology is the differential diagnosis of hemorrhages into the human brain substance (HBS) of traumatic and non-traumatic genesis. Objectives. This study aims to identify new criteria for objective forensic differentiation of hemorrhages of traumatic origin, cerebral infarction of ischemic and hemorrhagic genesis by using the method of complex degree of mutual anisotropy. For this study native sections of HBS from 125 corpses were used in the case of: death from coronary heart disease - 35 (28%) of native sections (Group 1 - control); hemorrhages of traumatic genesis - 30 (24%) sections (Group 2); ischemic cerebral infarction - 30 (24%) native sections(Group 3); and hemorrhages of non-traumatic genesis - 30 (24%) native sections (Group 4). Results. The statistical moments of the third and fourth orders, which characterize the asymmetry and excess of the complex degree of mutual anisotropy module size distributions, the strength of the method of polarization-correlation microscopy in the differentiation of the samples of the histological sections of the brain of control and experimental groups reached a good level — 87%-90%. Conclusion. The method of complex degree of mutual anisotropy allows differentiating with great precision the genesis of hemorrhage into the substance of the brain.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Methods to Determine Complex Degrees of Mutual Anisotropy for the Differentiation of Hemorrhages in the Human Brain Substance Resulting From Traumatic and Non-Traumatic Genesis

Garazdiuk

Bachynskіy

Nechytailo

et al. 2020

PMP

View full text Add to dashboard Cite

show abstract

“…The basic principle of our modeling process was based on the use of microscopic images of biological layers of human tissue and fluid, which were well-established Müller-matrix polarimetric analytical approach for the diagnosis of morphological and molecular work of BT. (Ushenko et al, 2016). According to this approach, the biological object is considered as a twocomponent ("isotropic-anisotropic") structural matrix.…”

Section: Methodsmentioning

confidence: 99%

Estimation of the Time Since Death by Using the Polarization Muller-Matrix Microscopy of Biological Tissue in Cases of Alcohol and Carbon Monoxide Poisoning

Ivaskevich

Vanchuliak

Bachynskіy

et al. 2020

PMP

View full text Add to dashboard Cite

Introduction: This paper aims to investigate and substantiate the information capabilities of the objective method of azimuthal-invariant polarization Müller-matrix microscopy of histological sections of biological tissues (BT) in the diagnosis of time since death (TSD) in alcohol and monoxide poisoning. Objectives: The purpose of the study was to evaluate the diagnostic capabilities of the azimuthal-invariant Muller-matrix polarimetry technique of the polycrystalline constituent of BT and human body fluids in determining the TSD in cases of alcohol and carbon monoxide poisoning. Results: A comprehensive experimental study of the diagnostic efficiency of the azimuthal-invariant Muller-matrix polarimetric microscopy of the polycrystalline component of histological sections of the brain, myocardium, adrenal glands, liver, and polycrystalline blood films from dead persons for the diagnosis of TSD in cases of alcohol and carbon monoxide poisoning.A sensitivity range of 24 hours was determined using the method of the azimuthal-invariant Muller-matrix polarimetric microscopy of the polycrystalline component of histological sections of the myocardium for the diagnosis of TSD in cases of alcohol and carbon monoxide poisoning. Conclusion: The results showed the effectiveness of the studied methodology and the prospect of further research in this direction.

show abstract

“…Stage 4. Synthesis of model-classifiers Model-classifiers were built using the multi-row algorithm of the GMDH [13]. Several algorithms for the synthesis of models implemented in the MIS were sequentially tested.…”

Section: Multidimensional Polarization Microscopy Of Histological Secmentioning

confidence: 99%

“…Now more than 20 algorithms for synthesis of models (ASM) were implemented in the synthesizer of MIS. These are the basic algorithms of the GMDH [13], GMDH-like algorithms, neural networks of various topologies, genetic and hybrid algorithms, etc. The synthesizer provides a choice of the algorithm for synthesis of models adaptively to the properties of the AID.…”

Section: Introductionmentioning

confidence: 99%

Classification of Multidimensional Polarization Microscopy Results in the Technology of Forensic Intellectual Monitoring of Heart Diseases

Vanchulyak

Golub

Talakh

et al. 2020

IAPGOS

View full text Add to dashboard Cite

The work combines methods of multidimensional polarization microscopy, statistical processing of data and inductive modeling with the purpose of constructing a methodology for creation of intelligent systems for multi-level forensic medical monitoring based on the example of the post-mortem diagnosis of coronary heart disease and acute coronary insufficiency. The task of classifying the results of the study of biological materials for obtaining a diagnosis was solved. To obtain informative features, a model of biological tissue of the myocardium was developed and the main diagnostic parameters were determined (statistical moments of 1–4 orders of coordinate distributions of the values of azimuths and the ellipticity of polarization and their autocorrelation functions, as well as wavelet coefficients of the corresponding distributions), which are dynamic due to its necrotic changes. The classification of these data was provided by constructing a deciding rule in the multi – raw algorithm of the GMDH. The effectiveness of the described methodology has been experimentally proved.

show abstract

Mueller-matrix mapping of optically anisotropic fluorophores of molecular biological tissues in the diagnosis of death causes

Cited by 7 publications

References 22 publications

Methods to Determine Complex Degrees of Mutual Anisotropy for the Differentiation of Hemorrhages in the Human Brain Substance Resulting From Traumatic and Non-Traumatic Genesis

Methods to Determine Complex Degrees of Mutual Anisotropy for the Differentiation of Hemorrhages in the Human Brain Substance Resulting From Traumatic and Non-Traumatic Genesis

Estimation of the Time Since Death by Using the Polarization Muller-Matrix Microscopy of Biological Tissue in Cases of Alcohol and Carbon Monoxide Poisoning

Classification of Multidimensional Polarization Microscopy Results in the Technology of Forensic Intellectual Monitoring of Heart Diseases

Contact Info

Product

Resources

About