Multi-analyte SPR immunoassays for environmental biosensing of pesticides

Abstract

Multi-analyte detection of environmentally relevant pesticides is performed by using a two-channelled surface plasmon resonance (SPR) biosensor. The special design of the SPR instrument allows the determination of several analytes (DDT, chlorpyrifos and carbaryl) via different immobilization formats. First, simultaneous pesticide monitoring is possible by flowing chlorpyrifos, carbaryl or DDT samples separately over each channel of the SPR system, wherein their corresponding recognition element was previously immobilized. The second approach is based on the multiple and combined immobilization of several analyte recognition elements on the sensing surface of one individual flow cell. In this format, the analysis time for all three pesticides varied from 40 to 60 min depending on the number of regeneration cycles. In most cases, similar detection limits were attained for the target analyte irrespective of the assay format, with sensitivity values at the nanogram per litre level (18-50 ng L(-1)). The assay reproducibility was proved through the repeated use of the same sensor surface for over more than 200 assay cycles, whereas the absence of biosensor response to non-related analytes showed the specificity and reliability of the analysis. The SPR instrument, including optics, electronics and microfluidics, is already commercialised by the company SENSIA, SL.

Au/Fe/Au multilayer transducers for magneto-optic surface plasmon resonance sensing

“…These features turn the MOSPR biosensor into a simple solution to improve the LOD of SPR biosensors, which could be of paramount utility for high sensitivity applications, as for example in clinical diagnostics, 20,21 food safety, 22 and environmental protection. 23 In order to surpass the sensing features of the SPR sensor, the MP transducer must have a large MO activity and a well-defined SPR; i.e., a sharp and angular narrow resonance. In a previous work, a Au/Cr/Co multilayer was employed as MOSPR transducer.…”

Immunodiagnostics and Immunosensor Design

“…The label-free transducer principles are typically based on a variety of responses, including increase of mass-induced mechanical deflection (cantilever) [78,79] shown in Fig. 8, mass spectrometry [80], mass-induced change of gold/silver surface light reflection surface plasmon resonance [81], electrical potential changes [82,83], change of the inner heat (calorimetry) [84], acoustic or surface acoustic wave detection [85], surface-enhanced Raman scattering [86], and piezoelectric or piezoresistive platforms [87,88], among others (Table 1). Label-free detection methods have been used primarily for in-depth characterization of biomolecule interactions.…”

Application of Modern Biosensors Methods in Ecotoxicological Monitoring of Some Toxins of Natural (Micotoxins) and Antropogenic (Pesticides) Origin. Part 2. Pesticides

“…В якості селективних молекул кла-сично використовують специфічні антитіла. Так, комерційний Імуносенсор ППР (SENSIA) використовували для одночасного виявлення ДТТ, хлорпірифосу та карбарилу, показуючи добру відтворюваність результатів (близько 200 випробувань) [81]. Імуносенсор на осно-ві повної внутрішньої флуоресценції відбит-тя (TIRF), "названий річковим аналізатором" (RIANA), дозволив визначати атразін, сімазін та алахлор у зразках води з чутливістю, що відповідає гранично допустимій концентрації, встановленій директивою Європейського Со-юзу для питної води [82].…”