Multi-approach analysis to assess the chromium(III) immobilization by Ochrobactrum anthropi DE2010

Villagrasa, Eduard; Ballesteros, Belén; Obiol, Aleix; Millach, Laia; Esteve, Isabel; Solé, Antonio

doi:10.1016/j.chemosphere.2019.124663

Cited by 12 publications

(7 citation statements)

References 58 publications

(57 reference statements)

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“…In a previous study, we demonstrated the colocalisation of Cr(III) with intracytoplasmic polyP inclusions using scanning transmission electron microscopy (STEM) coupled with EDX (Villagrasa et al 2020). Nevertheless, the abundance of these inclusions in relation with the presence of chromium was not considered.…”

Section: Electron Microscopymentioning

confidence: 98%

“…O. anthropi DE2010 is a gram-negative, nonspore, rod shaped, marine, heterotrophic bacterium. In addition, O. anthropi DE2010 immobilises Cr(III) in cytoplasmic inclusions of polyP (Villagrasa et al 2020). Under conditions of nutrient starvation and stress, such as the presence of heavy metals, some microorganisms can accumulate polyP via gene-regulated mechanisms (Baxter and Jensen 1980;Jensen et al 1986;Kuroda et al 2001;Narancic et al 2012;Burgos et al 2013;Millach et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Genomic and biotechnological insights on stress-linked polyphosphate production induced by chromium(III) in Ochrobactrum anthropi DE2010

Villagrasa

Egea

Ferrer-Miralles

et al. 2020

World J Microbiol Biotechnol

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The resistance of microorganisms to heavy metals in polluted environments is mediated by genetically determined mechanisms. One such mechanism includes the intracellular sequestration of heavy metals in polyphosphate (polyP) inclusions. In Cr(III) contaminated mediums, Ochrobactrum anthropi DE2010 is able to bind and sequester Cr(III) in polyP inclusions. In order to further study the relationship between Cr(III) tolerance and polyP production in O. anthropi DE2010, we carried out whole genomic sequencing, analysis of single nucleotide polymorphisms (SNPs), polyP chemical quantification, and determination of the relative abundance and morphometry of polyP inclusions. In the O. anthropi DE2010 genome, six polyP and pyrophosphate (PPi) metabolic genes were found. Furthermore, genomic analysis via SNPs calling revealed that O. anthropi ATCC49188 and DE2010 strains had average variations of 1.51% in their whole genome sequences and 1.35% variation associated with the principal polyP metabolic gene cluster. In addition, the accumulation of polyP in the DE2010 strain and number of polyP inclusions found were directly correlated with the concentration of Cr(III) in contaminated cultures. The results presented in this study may enhance the understanding of polyP production in response to Cr(III) toxicity in the O. anthropi DE2010 strain. This knowledge may facilitate the successful removal of Cr(III) from the natural environment.

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Section: Electron Microscopymentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Genomic and biotechnological insights on stress-linked polyphosphate production induced by chromium(III) in Ochrobactrum anthropi DE2010

Villagrasa

Egea

Ferrer-Miralles

et al. 2020

World J Microbiol Biotechnol

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“…Among many beneficial environmental bacterial strains, the ones belonging to the Ochrobactrum genus are of particular interest to environmental scientists due to their potential application in the bioremediation of many organic and inorganic micropollutants [ 31 , 32 , 33 ]. The ability of Ochrobactrum spp.…”

Section: Introductionmentioning

confidence: 99%

“…The ability of Ochrobactrum spp. to reduce chlorate [ 34 ], remove polychlorinated biphenyls [ 35 ] and toxic metals such as Cd (II) [ 36 ] or Cd (III) [ 31 ] from the environment has been proved. Ochrobactrum anthropi , a Gram-negative, heterotrophic bacterium is abundant in the environment.…”

Section: Introductionmentioning

confidence: 99%

“…Ochrobactrum anthropi , a Gram-negative, heterotrophic bacterium is abundant in the environment. Various strains of O. anthropi have been recently isolated from many environmental compartments globally, for example, activated sludge collected from WWTPs in China [ 34 , 37 ], microbial mats collected from the delta region of the Ebro River [ 31 ] or sediment harvested from the surrounding of former chemical factory in Slovakia [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

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Evaluating the Effect of Azole Antifungal Agents on the Stress Response and Nanomechanical Surface Properties of Ochrobactrum anthropi Aspcl2.2

2020

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Azole antifungal molecules are broadly used as active ingredients in various products, such as pharmaceuticals and pesticides. This promotes their release into the natural environment. The detailed mechanism of their influence on the biotic components of natural ecosystems remains unexplored. Our research aimed to examine the response of Ochrobactrum anthropi AspCl2.2 to the presence of four azole antifungal agents (clotrimazole, fluconazole, climbazole, epoxiconazole). The experiments performed include analysis of the cell metabolic activity, cell membrane permeability, total glutathione level and activity of glutathione S-transferases. These studies allowed for the evaluation of the cells’ oxidative stress response to the presence of azole antifungals. Moreover, changes in the nanomechanical surface properties, including adhesive and elastic features of the cells, were investigated using atomic force microscopy (AFM) and spectrophotometric methods. The results indicate that the azoles promote bacterial oxidative stress. The strongest differences were noted for the cells cultivated with fluconazole. The least toxic effect has been attributed to climbazole. AFM observations unraveled molecular details of bacterial cell texture, structure and surface nanomechanical properties. Antifungals promote the nanoscale modification of the bacterial cell wall. The results presented provided a significant insight into the strategies used by environmental bacterial cells to survive exposures to toxic azole antifungal agents.

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Characterization of two chromate reducing bacteria isolated from heavy metal contaminated soil

et al. 2021

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Multi-approach analysis to assess the chromium(III) immobilization by Ochrobactrum anthropi DE2010

Cited by 12 publications

References 58 publications

Genomic and biotechnological insights on stress-linked polyphosphate production induced by chromium(III) in Ochrobactrum anthropi DE2010

Genomic and biotechnological insights on stress-linked polyphosphate production induced by chromium(III) in Ochrobactrum anthropi DE2010

Evaluating the Effect of Azole Antifungal Agents on the Stress Response and Nanomechanical Surface Properties of Ochrobactrum anthropi Aspcl2.2

Characterization of two chromate reducing bacteria isolated from heavy metal contaminated soil

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