Multi-carbon dots and aptamer based signal amplification ratiometric fluorescence probe for protein tyrosine kinase 7 detection

Ma, Yunsu; Wang, Yuan; Liu, Yongjie; Shi, Lujia; Yang, Dongzhi

doi:10.1186/s12951-021-00787-7

Cited by 33 publications

(11 citation statements)

References 45 publications

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“…Figure 2 d (upper panel) shows the normalized absorption and emission spectra of a series of CPNs without PtOEP. The absorption spectra of CPNs decorated with aptamers show increased absorption around 255–260 nm, which is associated to the presence of aptamers [ 34 , 35 ]. The shape and intensity of emission spectra is identical for all samples.…”

Section: Resultsmentioning

confidence: 99%

Selective Photo-Assisted Eradication of Triple-Negative Breast Cancer Cells through Aptamer Decoration of Doped Conjugated Polymer Nanoparticles

et al. 2022

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Photodynamic therapy (PDT) may be an excellent alternative in the treatment of breast cancer, mainly for the most aggressive type with limited targeted therapies such as triple-negative breast cancer (TNBC). We recently generated conjugated polymer nanoparticles (CPNs) as efficient photosensitizers for the photo-eradication of different cancer cells. With the aim of improving the selectivity of PDT with CPNs, the nanoparticle surface conjugation with unique 2’-Fluoropyrimidines-RNA-aptamers that act as effective recognition elements for functional surface signatures of TNBC cells was proposed and designed. A coupling reaction with carbodiimide was used to covalently bind NH2-modified aptamers with CPNs synthetized with two polystyrene-based polymer donors of COOH groups for the amide reaction. The selectivity of recognition for TNBC membrane receptors and PDT efficacy were assayed in TNBC cells and compared with non-TNBC cells by flow cytometry and cell viability assays. Furthermore, in vitro PDT efficacy was assayed in different TNBC cells with significant improvement results using CL4, sTN29 and sTN58 aptamers compared to unconjugated CPNs and SCR non-specific aptamer. In a chemoresistance TNBC cell model, sTN58 was the candidate for improving labelling and PDT efficacy with CPNs. We proposed sTN58, sTN29 and CL4 aptamers as valuable tools for selective TNBC targeting, cell internalization and therapeutic improvements for CPNs in PDT protocols.

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Section: Resultsmentioning

confidence: 99%

Selective Photo-Assisted Eradication of Triple-Negative Breast Cancer Cells through Aptamer Decoration of Doped Conjugated Polymer Nanoparticles

et al. 2022

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“…Ma et al, designed a fluorescent probe for detecting protein tyrosine kinase 7 in the peripheral circulation using multi-carbon dots and an aptamer-based signal amplification ratiometric. As a result, its presence in the peripheral circulation might help in cancer diagnosis (Ma et al, 2021a).…”

Section: Applications Of Quantum Dots In Cancer Treatment Early Diagnosis Of Cancer By Quantum Dotsmentioning

confidence: 99%

Quantum Dots: An Emerging Approach for Cancer Therapy

Devi¹,

Kumar

Tiwari

et al. 2022

Front. Mater.

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Nanotechnology is indisputably a scientific technique that offers the prospect of new therapies, and hope, for the treatment of malignant illnesses. It is a novel technology that offers new approaches for the diagnosis and management of diverse diseases. Although the discovery of Quantum dots (QD) nano-transporters has already led to a few positive developments, QD nano-transporters are still at their initial stage, though have yet proven valuable to society. The excertion of QD indicates conversion in natural imaging along with photograph have established incredible suitability in bio-imaging, new drug development, targeted gene deliverance, biosensing, photodynamic treatment as well as diagnosis. The present review aimed to confer the significance of QD in diagnosis as well as in management of cancer. This review aims to impart fundamental insight as well as conception of QD its merits, properties, utilization as well as mode of action. This review highlight of different designing schemes of QD like hydrothermal, drop-casting, ultrasonic, solvothermal, spin-coating, atomic layer desorption, layer by layer, polymethylmethacrylate aided-transfer, electrochemical, ion beam sputtering deposition. Moreover, we have elaborated on the diverse researches related to cytotoxic examination to reveal that QDs are harmless. Concisely, the present review summarizes the fabrication schemes, current research and utilization of QD in cancer treatment.

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“…However, and a variety of factors can in uence measurement quality, including probe concentration, photosource and experimental conditions [8]. It has also been reported that some ratiometric uorescent probes consist of two or more uorescent materials [9][10][11][12]. In contrast to the conventional uorescence method that utilizes a single uorophore, ratiometric uorescence determination method consisted of multiple uorophores offers increased selectivity and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

A Convenient Ratiometric fluorescent Probe Based on Gold Nanoclusters and Carbon Dots for Escherichia coli Determination

Liu,

Wang,

Liu

et al. 2023

Preprint

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Escherichia coli, as a prevalent foodborne pathogen, can harm people health seriously. Hence, developing the effective methods for Escherichia coli (E. coli) determination is vital. In the paper, a convenient hybridization ratiometric fluorescent probe was constructed for the prompt determination of E.coli based on red gold nanoclusters (AuNCs) and blue carbon dots (bCDs). The relevant experimental conditions were systematically optimized including copper concentration, incubation temperature and time, in order to obtain the optimal results. There existed a strong linear relationship between the fluorescence intensity ratios (I630/I450) and the concentrations of E. coli for both AuNCs and bCDs. Based on the determination, it was found that the linear concentration range spanned from 103 to 107 CFU*mL− 1, with a low limit of detection (LOD) of 120 CFU*mL− 1. This method based on the mechanism of copper cocatalysis presents a sensitive and convenient strategy for E.coli determination, and has a promising future in the field of food safety.

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Multi-carbon dots and aptamer based signal amplification ratiometric fluorescence probe for protein tyrosine kinase 7 detection

Cited by 33 publications

References 45 publications

Selective Photo-Assisted Eradication of Triple-Negative Breast Cancer Cells through Aptamer Decoration of Doped Conjugated Polymer Nanoparticles

Selective Photo-Assisted Eradication of Triple-Negative Breast Cancer Cells through Aptamer Decoration of Doped Conjugated Polymer Nanoparticles

Quantum Dots: An Emerging Approach for Cancer Therapy

A Convenient Ratiometric fluorescent Probe Based on Gold Nanoclusters and Carbon Dots for Escherichia coli Determination

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