“Multi-Cloistered” NMR Cells

Banas, E. M.

doi:10.1366/000370269774380833

Cited by 6 publications

(2 citation statements)

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“…Microcoils provide another avenue to increase NMR throughput via parallel NMR detection. − Because solenoidal microcoils are aligned perpendicularly to the magnetic field and are smaller than most saddle-shaped Helmholtz coils, multiple microcoils can be stacked along the magnetic field axis in a single NMR probe. Parallel analysis is common for other analytical techniques but has only recently been explored for NMR. − Various approaches have been attempted, including isolated circuits, , rapid selective sample excitation, and imaging methods. , We previously introduced the multiple-coil Multiplex NMR probe for parallel NMR analysis, which is capable of analyzing four samples using chemical shift imaging or, in rapid succession, using a selective excitation approach . In this article we present an automated injection system incorporating an enhanced version of our Multiplex NMR probe and a Gilson multiprobe 215 liquid handler.…”

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“…Parallel analysis is common for other analytical techniques but has only recently been explored for NMR. [26][27][28][29][30][31][32][33][34] Various approaches have been attempted, including isolated circuits, 26,28 rapid selective sample excitation, 32 and imaging methods. 32,34 We previously introduced the multiple-coil Multiplex NMR probe for parallel NMR analysis, which is capable of analyzing four samples using chemical shift imaging or, in rapid succession, using a selective excitation approach.…”

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High-Throughput Nuclear Magnetic Resonance Analysis Using a Multiple Coil Flow Probe

Macnaughtan

Hou

et al. 2003

Anal. Chem.

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An automated method for high-throughput nuclear magnetic resonance (NMR) spectroscopy has been developed using a four-coil Multiplex NMR probe. The probe is constructed with solenoidal microcoils optimized for detection of small volume, mass-limited samples and a flow-through design. Four samples can be simultaneously injected into the Multiplex probe with a robotics liquid handler and then analyzed in rapid succession using a selective excitation experiment. Due to the simultaneous injection of four samples and the reduced analysis time with rapid selective excitation, the analysis rate achieved thus far is as low as 1 sample/34 s for 1D 1H NMR.

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High-Throughput Nuclear Magnetic Resonance Analysis Using a Multiple Coil Flow Probe

Macnaughtan

Hou

et al. 2003

Anal. Chem.

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High‐throughput screening of chemical exchange saturation transfer MR contrast agents

Liu

Gilad

Bulte

et al. 2010

Contrast Media & Molecular

105

153

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A new high throughput (HT) MRI method for screening Chemical Exchange Saturation Transfer (CEST) agents is demonstrated, allowing simultaneous testing of multiple samples with minimal attention to sample configuration and shimming of the main magnetic field (B 0 ). This approach, which is applicable to diamagnetic (DIACEST), paramagnetic (PARACEST) and liposome (LIPOCEST) CEST agents, employs a set of inexpensive glass or plastic capillary tubes containing CEST agents put together in a cheap plastic tube holder, without need for liquid between the tubes to reduce magnetic susceptibility effects. In this setup, a image of direct water saturation spectra is acquired in order to map the absolute water frequency for each volume element (voxel) in the sample image, followed by an image of saturation transfer spectra to determine the CEST properties. Even though the field over the total sample is very inhomogeneous due to air/tube interfaces, the shape of the direct saturation spectra is not affected, allowing removal of susceptibility shift effects from the CEST data by using the absolute water frequencies from the map. As a result, quantitative information such as the mean CEST intensity for each sample can be extracted for multiple CEST agents at once. As an initial application, we demonstrate rapid screening of a library of 16 polypeptides for their CEST properties, but in principle the number of tubes is limited only by the available signal-noiseratio, field of view and gradient strength for imaging.

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Recent advances in nuclear magnetic resonance spectroscopy detection compatible with on-flow operational regimes: New uses of NMR detectors hyphenated to LCs and other separation techniques and in reaction monitoring

Salgado

2023

Liquid Chromatography

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“Multi-Cloistered” NMR Cells

Cited by 6 publications

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High-Throughput Nuclear Magnetic Resonance Analysis Using a Multiple Coil Flow Probe

High-Throughput Nuclear Magnetic Resonance Analysis Using a Multiple Coil Flow Probe

High‐throughput screening of chemical exchange saturation transfer MR contrast agents

Recent advances in nuclear magnetic resonance spectroscopy detection compatible with on-flow operational regimes: New uses of NMR detectors hyphenated to LCs and other separation techniques and in reaction monitoring

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