Multi-color ultrafast laser platform for nonlinear optical imaging based on independently tunable optical parametric oscillators

Yang, Shan; Adhikari, Sanjay; Dobbala, Manoj; Adusumilli, Sasidhar; Rowley, J.; Ganikhanov, Feruz; Zhang, Lingquin; Marrs, Glen S.; Wysolmerski, Robert B.; Spirou, George A.

doi:10.1007/s00340-013-5381-x

Cited by 11 publications

(11 citation statements)

References 37 publications

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“…Image stacks (Z-step size 0.7-1.2 m) were collected using either a Zeiss LSM510 confocal microscope or custom-built 2P microscope (Yang et al, 2013). Confocal microscopy was performed using a 20ϫ (0.75 numerical aperture) PLAN NEOFLOUR lens.…”

Section: Methodsmentioning

confidence: 99%

Synaptic Inputs Compete during Rapid Formation of the Calyx of Held: A New Model System for Neural Development

Holcomb

Hoffpauir

Hoyson

et al. 2013

Journal of Neuroscience

111

197

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Hallmark features of neural circuit development include early exuberant innervation followed by competition and pruning to mature innervation topography. Several neural systems, including the neuromuscular junction and climbing fiber innervation of Purkinje cells, are models to study neural development in part because they establish a recognizable endpoint of monoinnervation of their targets and because the presynaptic terminals are large and easily monitored. We demonstrate here that calyx of Held (CH) innervation of its target, which forms a key element of auditory brainstem binaural circuitry, exhibits all of these characteristics. To investigate CH development, we made the first application of serial block-face scanning electron microscopy to neural development with fine temporal resolution and thereby accomplished the first time series for 3D ultrastructural analysis of neural circuit formation. This approach revealed a growth spurt of added apposed surface area (ASA) Ͼ200 m 2 /d centered on a single age at postnatal day 3 in mice and an initial rapid phase of growth and competition that resolved to monoinnervation in two-thirds of cells within 3 d. This rapid growth occurred in parallel with an increase in action potential threshold, which may mediate selection of the strongest input as the winning competitor. ASAs of competing inputs were segregated on the cell body surface. These data suggest mechanisms to select "winning" inputs by regional reinforcement of postsynaptic membrane to mediate size and strength of competing synaptic inputs.

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Section: Methodsmentioning

confidence: 99%

Synaptic Inputs Compete during Rapid Formation of the Calyx of Held: A New Model System for Neural Development

Holcomb

Hoffpauir

Hoyson

et al. 2013

Journal of Neuroscience

111

197

View full text Add to dashboard Cite

show abstract

“…Other details and characteristics of the set up are described in our most recent work. 20 Figure 2(a) demonstrates sensitivity and the attainable time resolution using the experimental arrangement. In addition, using theoretical algorithms and owing to the experiment's great sensitivity, we can retrieve the vibrational system's response function and Raman spectra for several vibrational modes.…”

mentioning

confidence: 99%

“…In our studies we employed three-color CARS geometry with widely tunable 110-150 fs pulses. 19,20 The experimental set up is schematically shown in Figure 1. The two pulses that are needed to coherently drive lattice vibrations within a sample's macroscopic volume are provided by synchronously pumped optical parametric oscillators (OPOs) running at 76 MHz.…”

mentioning

confidence: 99%

Resolving fine spectral features in lattice vibrational modes using femtosecond coherent spectroscopy

2016

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We show resolution of fine spectral features within several Raman active vibrational modes in potassium titanyl phosphate (KTP) crystal. Measurements are performed using a femtosecond time-domain coherent anti-Stokes Raman scattering spectroscopy technique that is capable of delivering equivalent spectral resolution of 0.1 cm−1. The Raman spectra retrieved from our measurements show several spectral components corresponding to vibrations of different symmetry with distinctly different damping rates. In particular, linewidths for unassigned optical phonon mode triplet centered at around 820 cm−1 are found to be 7.5 ± 0.2 cm−1, 9.1 ± 0.3 cm−1, and 11.2 ± 0.3 cm−1. Results of our experiments will ultimately help to design an all-solid-state source for sub-optical-wavelength waveform generation that is based on stimulated Raman scattering.

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“…Four images were taken in varying locations throughout each collagen gel piece. The total depth of the collagen gel was measured using twophoton fluorescent microscopy (Yang et al, 2011(Yang et al, , 2013 by imaging the fluorescence to determine the top and bottom of the gel.…”

Section: Gel Compression Studiesmentioning

confidence: 99%

“…Collagen gels were washed well with PBS and coverslipped with 90% glycerol/10% PBS containing 0.1 M n-propyl gallate (Giloh and Sedat, 1982). Z-stack images of the collagen gel were generated by taking 1.5 µm steps through the total depth of the gel using two-photon fluorescent microscopy (Yang et al, 2011(Yang et al, , 2013.…”

Section: Focal Adhesion Immunostaining In Three-dimensional (3-d) Colmentioning

confidence: 99%

The role of focal adhesion kinase in nonmuscle cell contraction

Arnold

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Multi-color ultrafast laser platform for nonlinear optical imaging based on independently tunable optical parametric oscillators

Cited by 11 publications

References 37 publications

Synaptic Inputs Compete during Rapid Formation of the Calyx of Held: A New Model System for Neural Development

Synaptic Inputs Compete during Rapid Formation of the Calyx of Held: A New Model System for Neural Development

Resolving fine spectral features in lattice vibrational modes using femtosecond coherent spectroscopy

The role of focal adhesion kinase in nonmuscle cell contraction

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