Multi-contrast computed laminography at ANKA light source

Cheng, Yin; Altapova, Venera; Helfen, Lukas; Xu, Feng; Rolo, Tomy dos Santos; Vagovič, Patrik; Fiederle, M.; Baumbach, Tilo

doi:10.1088/1742-6596/463/1/012038

Cited by 10 publications

(5 citation statements)

References 17 publications

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“…To improve contrast, we optimized the sample preparation protocol with respect to fixation, staining and embedding. About 2000 X-ray projection images per tomogram were acquired at the high resolution tomography end station of the synchrotron light source facility at KIT 53 , 54 . To avoid deterioration of specimens only freshly fixed and stained samples were used and storage of samples was avoided.…”

Section: Methodsmentioning

confidence: 99%

Quantitative morphometric analysis of adult teleost fish by X-ray computed tomography

Weinhardt¹,

Shkarin²,

Wernet³

et al. 2018

Sci Rep

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Vertebrate models provide indispensable paradigms to study development and disease. Their analysis requires a quantitative morphometric study of the body, organs and tissues. This is often impeded by pigmentation and sample size. X-ray micro-computed tomography (micro-CT) allows high-resolution volumetric tissue analysis, largely independent of sample size and transparency to visual light. Importantly, micro-CT data are inherently quantitative. We report a complete pipeline of high-throughput 3D data acquisition and image analysis, including tissue preparation and contrast enhancement for micro-CT imaging down to cellular resolution, automated data processing and organ or tissue segmentation that is applicable to comparative 3D morphometrics of small vertebrates. Applied to medaka fish, we first create an annotated anatomical atlas of the entire body, including inner organs as a quantitative morphological description of an adult individual. This atlas serves as a reference model for comparative studies. Using isogenic medaka strains we show that comparative 3D morphometrics of individuals permits identification of quantitative strain-specific traits. Thus, our pipeline enables high resolution morphological analysis as a basis for genotype-phenotype association studies of complex genetic traits in vertebrates.

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Section: Methodsmentioning

confidence: 99%

Quantitative morphometric analysis of adult teleost fish by X-ray computed tomography

Weinhardt¹,

Shkarin²,

Wernet³

et al. 2018

Sci Rep

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“…However, the inner microstructure evolution was inaccessible and little evidence was provided on the proposed crack propagation criteria. In this study, thanks to the synchrotron computed laminography (SRμCL), it has been possible to trace the volumetric micro‐crack initiation and propagation in a 2 mm thick notched PA6 laminate under severe triaxial stresses. SRμCL is a nondestructive 3D X‐ray imaging technique that allows for the assessment of the inner microstructure of a plate‐like object.…”

Section: Introductionmentioning

confidence: 99%

3D Damage Micromechanisms in Polyamide 6 Ahead of a Severe Notch Studied byIn SituSynchrotron Laminography

Cheng

Laiarinandrasana

Helfen

et al. 2016

Macromol. Chem. Phys.

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Micro‐damage mechanisms in a semicrystalline polyamide 6 polymer are characterized by in situ synchrotron radiation computed laminography (SRCL) using compact‐tension like specimens with a notch root radius of 0.25 mm. SRCL allows the quantification of cavity nucleation, growth, and coalescence in flat 2 mm thick notched specimens with a micrometer resolution in 3D (under conditions of severe stress triaxiality) during the in situ loading. The maximum damage occurred at mid‐thickness and was located at a small distance ≈ 200 µm from the notch root. It is shown that damage is distributed in distinct zones, which can be linked to different stress triaxiality states. Penny‐shaped cracks are found after some loadings and their diameters are a function of the distance to the notch root. In a layer of about 33 µm no damage was found. In the adjacent 128 µm layer, penny‐shaped cracks are found with diameters of the order of average spherulite sizes (4–6 µm). In the next region, 30–45 µm long cracks are found corresponding to several average spherulite diameters. During propagation, tunnel‐like cracks are formed ahead of the main crack. Finally, crack initiation location is found to be largely dependent on the roughness of the machined notch surface by comparing two samples with the same loading conditions.

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“…With the development of the SRCL technique at ANKA (Synchrotron Radiation Facility at the Karlsruhe Institute of Technology (KIT)), an increasing number of scientific publications are found in literature. The SRCL technique was adopted to investigate the crack initiation of Al2139 [Morgeneyer et al, 2009[Morgeneyer et al, , 2013, the crack initiation and propagation in Al6061 aluminum alloy sheets [Shen et al, 2013] and the damage evolution in a polymer composite [Xu et al, 2010;Laiarinandrasana et al, 2012;Cheng et al, 2013b]. These scientific investigations confirm that the SRCL technique is qualified for 3D imaging of the damage evolution of different materials.…”

Section: Synchrotron Radiation-computed Laminography (Srcl)mentioning

confidence: 76%

Numerical Simulation and Experimental Investigation of the Fracture Behaviour of an Electron Beam Welded Steel Joint

Tu¹

2018

Springer Theses

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Multi-contrast computed laminography at ANKA light source

Cited by 10 publications

References 17 publications

Quantitative morphometric analysis of adult teleost fish by X-ray computed tomography

Quantitative morphometric analysis of adult teleost fish by X-ray computed tomography

3D Damage Micromechanisms in Polyamide 6 Ahead of a Severe Notch Studied byIn SituSynchrotron Laminography

Numerical Simulation and Experimental Investigation of the Fracture Behaviour of an Electron Beam Welded Steel Joint

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