Multi-enzyme activity of three layers FeO @ZnMnFeO @Fe-Mn organogel for colorimetric detection of antioxidants and norfloxacin with smartphone

Sun, Mengmeng; He, Mingxia; Shao-juan, Jiang; Wang, Yanying; Wang, Xianxiang; Liu, Tao; Song, Chang; Wang, Suning; Rao, Hanbing; Lü, Zhiwei

doi:10.1016/j.cej.2021.131823

Cited by 47 publications

(19 citation statements)

References 74 publications

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“…To reveal their oxidase-like and peroxidase-like catalytic mechanisms, the types of free radical were verified by free radical scavenger and electron spin resonance (ESR) measurements. Generally, thiourea (TH), p -benzoquinone (PBQ), ethylene diamine tetraacetic acid (EDTA), and sodium azide (NaN 3 ) were used as the scavengers of the hydroxyl radical (·OH), superoxide anion (O 2 ·– ), oxygen vacancies, and singlet oxygen ( 1 O 2 ), respectively . Briefly, TH, NaN 3 , PBQ, and EDTA were separately mixed into an acetate buffer (0.2 M, pH 3) containing H 2 O 2 /TMB or TMB, and then Co 3 O 4 /CoFe 2 O 4 HNCs (1 mg·mL –1 ) were mixed into the aforementioned solution and reacted at 40 °C for 20 min.…”

Section: Methodsmentioning

confidence: 99%

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Co₃O₄/CoFe₂O₄ Hollow Nanocube Multifunctional Nanozyme with Oxygen Vacancies for Deep-Learning-Assisted Smartphone Biosensing and Organic Pollutant Degradation

Shao-juan

et al. 2023

ACS Appl. Mater. Interfaces

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Although the application of nanozymes has been widely studied, it is still a huge challenge to develop highly active and multifunctional nanozyme catalysts with a wider application prospect. Co 3 O 4 /CoFe 2 O 4 hollow nanocubes (HNCs) with oxygen vacancies were proposed in this study, which had a porous oxide heterostructure with CoFe 2 O 4 as the core and Co 3 O 4 as the shell. The Co 3 O 4 /CoFe 2 O 4 HNCs had three enzyme activities: peroxidase-like, oxidase-like, and catalase-like. Combining XPS depth profiling with density functional theory (DFT), the catalytic mechanism of peroxidase-like activity was explored in depth, which was mainly originated from •OH produced by the synergistic effect between the outer oxygen and inner oxygen and electron transfer between Co and Fe. A colorimetry/smartphone dual sensing platform was designed based on the peroxidase-like activity. Especially, a multifunctional intelligent sensing platform based on deep learning-YOLO v3 algorithm-assisted smartphone was constructed to realize real-time and rapid in situ detection of L-cysteine, norfloxacin, and zearalenone. Surprisingly, the detection limit of norfloxacin was low at 0.015 μM, which was better than that of the newly published detection method in the field of nanozymes. Meanwhile, the detection mechanism of L-cysteine and norfloxacin was successfully investigated by in situ FTIR. In fact, it also showed outstanding applications in detecting L-cysteine in the food environment and norfloxacin in drugs. Furthermore, Co 3 O 4 /CoFe 2 O 4 HNCs also could degrade 99.24% of rhodamine B, along with good reusability even after 10-cycle runs. Therefore, this work provided an in-depth understanding of the synergistic effect between the outer and inner oxygen in the reaction mechanism and an efficient method for establishing a deep-learning-assisted intelligent detection platform. In addition, this research also offered a good guideline for the further development and construction of nanozyme catalysts with multienzyme activities and multifunctional applications.

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Section: Methodsmentioning

confidence: 99%

“…•− ), oxygen vacancies, and singlet oxygen ( 1 O 2 ), respectively. 32 Briefly, TH, NaN were mixed with the acetate buffer solution (pH 3.5, 0.2 M) followed by addition of different concentrations of Cys solutions to maintain a total volume of 3 mL. After being reacted at 40 °C for 20 min, the absorbency was measured at 652 nm.…”

Section: Methodsmentioning

confidence: 99%

Co₃O₄/CoFe₂O₄ Hollow Nanocube Multifunctional Nanozyme with Oxygen Vacancies for Deep-Learning-Assisted Smartphone Biosensing and Organic Pollutant Degradation

Shao-juan

et al. 2023

ACS Appl. Mater. Interfaces

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“…This kind of combination can be used for POCT. Sun et al [ 65 ] synthesized a three-layer FeOx@ZnMnFeOy @Fe Mn bimetallic organism with excellent multienzyme activity (peroxidase, oxidase, and catalase). Therefore, this sensing platform can complete the four functions of detecting H 2 O 2 , citric acid (CA), norfloxacin (NOR), and gallic acid.…”

Section: Environmental Monitoringmentioning

confidence: 99%

Application of Nanozymes in Environmental Monitoring, Management, and Protection

et al. 2023

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Nanozymes are nanomaterials with enzyme-like activity, possessing the unique properties of nanomaterials and natural enzyme-like catalytic functions. Nanozymes are catalytically active, stable, tunable, recyclable, and versatile. Therefore, increasing attention has been paid in the fields of environmental science and life sciences. In this review, we focused on the most recent applications of nanozymes for environmental monitoring, environmental management, and environmental protection. We firstly introduce the tuning catalytic activity of nanozymes according to some crucial factors such as size and shape, composition and doping, and surface coating. Then, the application of nanozymes in environmental fields are introduced in detail. Nanozymes can not only be used to detect inorganic ions, molecules, organics, and foodborne pathogenic bacteria but are also involved in the degradation of phenolic compounds, dyes, and antibiotics. The capability of nanozymes was also reported for assisting air purification, constructing biofuel cells, and application in marine antibacterial fouling removal. Finally, the current challenges and future trends of nanozymes toward environmental fields are proposed and discussed.

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“…Similar to the substrate specificity of natural enzymes, the substrates in different luminescent catalytic reactions depend on different types of nanozymes. Although some reported nanozymes have multiple enzyme‐mimicking activities, only a single enzyme‐mimicking activity was often used in specific practical applications (Liu, Zhang, et al., 2020; Sun, He, et al., 2021). Researchers can select the preferred catalytic function of nanozymes by varying the catalytic conditions.…”

Section: Mechanisms Of Nldsmentioning

confidence: 99%

Nanozyme‐encoded luminescent detection for food safety analysis: An overview of mechanisms and recent applications

Sun²,

Huang

et al. 2022

Comp Rev Food Sci Food Safe

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With the rapid growth in global food production, delivery, and consumption, reformative food analytical techniques are required to satisfy the monitoring requirements of speed and high sensitivity. Nanozyme-encoded luminescent detections (NLDs) integrating nanozyme-based rapid detections with luminescent output signals have emerged as powerful methods for food safety monitoring, not only because of their preeminent performance in analysis, such as rapid, facile, low background signal, and ultrasensitive, but also due to their strong attractiveness for future sensing research. However, the lack of a full understanding of the fundamentals of NLDs for food safety detection technologies limits their further application. In this review, a systematic overview of the mechanisms of NLDs and their applications in the food industry is summarized, which covers the nanozyme-mimicking types and their luminescent signal generation mechanisms, as well as their applications in monitoring common foodborne contaminants. As demonstrated by previous studies, NLDs are bridging the gap to practical-oriented food analytical technologies and various opportunities to improve their food analytical performance to be considered in the future are proposed.

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Multi-enzyme activity of three layers FeO @ZnMnFeO @Fe-Mn organogel for colorimetric detection of antioxidants and norfloxacin with smartphone

Cited by 47 publications

References 74 publications

Co₃O₄/CoFe₂O₄ Hollow Nanocube Multifunctional Nanozyme with Oxygen Vacancies for Deep-Learning-Assisted Smartphone Biosensing and Organic Pollutant Degradation

Co₃O₄/CoFe₂O₄ Hollow Nanocube Multifunctional Nanozyme with Oxygen Vacancies for Deep-Learning-Assisted Smartphone Biosensing and Organic Pollutant Degradation

Application of Nanozymes in Environmental Monitoring, Management, and Protection

Nanozyme‐encoded luminescent detection for food safety analysis: An overview of mechanisms and recent applications

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Multi-enzyme activity of three layers FeO @ZnMnFeO @Fe-Mn organogel for colorimetric detection of antioxidants and norfloxacin with smartphone

Cited by 47 publications

References 74 publications

Co3O4/CoFe2O4 Hollow Nanocube Multifunctional Nanozyme with Oxygen Vacancies for Deep-Learning-Assisted Smartphone Biosensing and Organic Pollutant Degradation

Co3O4/CoFe2O4 Hollow Nanocube Multifunctional Nanozyme with Oxygen Vacancies for Deep-Learning-Assisted Smartphone Biosensing and Organic Pollutant Degradation

Application of Nanozymes in Environmental Monitoring, Management, and Protection

Nanozyme‐encoded luminescent detection for food safety analysis: An overview of mechanisms and recent applications

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Product

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Co₃O₄/CoFe₂O₄ Hollow Nanocube Multifunctional Nanozyme with Oxygen Vacancies for Deep-Learning-Assisted Smartphone Biosensing and Organic Pollutant Degradation

Co₃O₄/CoFe₂O₄ Hollow Nanocube Multifunctional Nanozyme with Oxygen Vacancies for Deep-Learning-Assisted Smartphone Biosensing and Organic Pollutant Degradation