Multi-faceted integrated omics analysis revealed parsley (Petroselinum crispum) as a novel dietary intervention in dextran sodium sulphate induced colitic mice

Jia, Huijuan; Aw, Wanping; Hanate, Manaka; Takahashi, Shoko; Saito, Kenji; Tanaka, Hiroshi; Tomita, Masaru; Kato, Harubumi

doi:10.1016/j.jff.2014.09.018

Cited by 23 publications

(14 citation statements)

References 26 publications

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“…The protein preparation, iTRAQ labeling, and nanoscale liquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS) analysis were conducted as described previously35. We conducted the protein identification and quantification for iTRAQ samples using ProteinPilot software (ver.…”

Section: Methodsmentioning

confidence: 99%

Eggshell membrane ameliorates hepatic fibrogenesis in human C3A cells and rats through changes in PPARγ-Endothelin 1 signaling

Jia

Saito

et al. 2014

Sci Rep

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Our previous nutrigenomic findings indicate that eggshell membrane (ESM) may prevent liver fibrosis. Here we investigated the effects and mechanisms underlying ESM intervention against liver injury by using DNA microarray analysis and comparative proteomics. In vitro hydrolyzed ESM attenuated the TGFβ1-induced procollagen production of human hepatocyte C3A cells and inhibited the expression of Endothelin 1 (EDN1) and its two receptors, and extracellular matrix components. In vivo male Wistar rats were allocated into a normal control group, a CCl4 group (hypodermic injection of 50% CCl4 2×/wk) and an ESM group (20 g ESM/kg diet with CCl4 injection) for 7 wks. Dietary ESM ameliorated the elevated activity of ALT/AST, oxidative stress and collagen accumulation in liver, accompanied by the down-regulated expression of Edn1 signaling and notable profibrogenic genes and growth factors as well as peroxisome proliferator-activated receptor gamma (PPARγ). Concomitantly, the decreased expressions of Galectin-1 and Desmin protein in the ESM group indicated the deactivation of hepatic stellate cells (HSCs). Through a multifaceted integrated omics approach, we have demonstrated that ESM can exert an antifibrotic effect by suppressing oxidative stress and promoting collagen degradation by inhibiting HSCs' transformation, potentially via a novel modulation of the PPARγ-Endothelin 1 interaction signaling pathway.

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Section: Methodsmentioning

confidence: 99%

Eggshell membrane ameliorates hepatic fibrogenesis in human C3A cells and rats through changes in PPARγ-Endothelin 1 signaling

Jia

Saito

et al. 2014

Sci Rep

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“…During the DSS challenge, the body weight, stool consistency, and fecal blood were recorded daily as criteria for the DAI 78 . The DAI was calculated by combining these three scores and then dividing the score by 3.…”

Section: Evaluation Of Disease Activity Index (Dai)mentioning

confidence: 99%

“…4.0, AB SCIEX) with 95% confidence against switchProt. We used the Paragon Algorithm in the ProteinPilot software for the peptide identification and isoform specific quantification 78 .…”

Section: Real-time Reverse Transcription Polymerase Chain Reaction (Rmentioning

confidence: 99%

Integrated omics profiling of dextran sodium sulfate-induced colitic mice supplemented with Wolfberry (Lycium barbarum)

Jia

Lyu

et al. 2020

npj Sci Food

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We used a multi-omics profiling approach to investigate the suppressive effects of 2% Wolfberry (WOL)-enriched diets on dextran sodium sulfate (DSS)-induced colitis in mice. It was observed that in mice fed the WOL diet, the disease activity index, colon shortening, plasma concentrations of matrix metalloproteinase-3 and relative mesenteric fat weight were significantly improved as compared to the DSS group. Results from colon transcriptome and proteome profiles showed that WOL supplementation significantly ameliorated the expression of genes and proteins associated with the integrity of the colonic mucosal wall and colonic inflammation. Based on the hepatic transcriptome, proteome and metabolome data, genes involved in fatty acid metabolism, proteins involved in inflammation and metabolites related to glycolysis were downregulated in WOL mice, leading to lowered inflammation and changes in these molecules may have led to improvement in body weight loss. The integrated nutrigenomic approach thus revealed the molecular mechanisms underlying the ameliorative effect of whole WOL fruit consumption on inflammatory bowel disease.

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“…The pH of the murine stomach (pH 3-4, depending on the fasting state) is within the range that is observed in humans (pH 1-5, depending on the fasting state) (McConnell, Basit, & Murdan, 2008) and the major digestive enzymes (pepsin, trypsin, chymotrypsin, lipases, amylases) are present in rodents and men. The mouse is therefore an appropriate, easy to handle and often used model system in order to address questions in diverse research fields like food and health, infection, drug efficacy and safety or probiotic use (Chen et al, 2014;Jia et al, 2014;Shi et al, 2012;Takeda et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of milk protein based microencapsulation on bacterial survival in simulated gastric juice versus the murine gastrointestinal system

Würth

Hörmannsperger

Wilke

et al. 2015

Journal of Functional Foods

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Multi-faceted integrated omics analysis revealed parsley (Petroselinum crispum) as a novel dietary intervention in dextran sodium sulphate induced colitic mice

Cited by 23 publications

References 26 publications

Eggshell membrane ameliorates hepatic fibrogenesis in human C3A cells and rats through changes in PPARγ-Endothelin 1 signaling

Eggshell membrane ameliorates hepatic fibrogenesis in human C3A cells and rats through changes in PPARγ-Endothelin 1 signaling

Integrated omics profiling of dextran sodium sulfate-induced colitic mice supplemented with Wolfberry (Lycium barbarum)

Protective effect of milk protein based microencapsulation on bacterial survival in simulated gastric juice versus the murine gastrointestinal system

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