Multi-fingerprint and quality control analysis of tea polysaccharides

Structural diversity requires individual optimization of ethanol concentration in polysaccharide precipitation

“…Natural polysaccharides are important biologically active components of many medicinal herbs, and have thus been attracting increasing multidisciplinary research interest [1]. Several challenges exist in this research field: crystal structure determination, quality control, in vivo detection, and molecular target determination [2][3][4][5]. However, before these challenges can be effectively tackled, a more fundamental problem must be addressed, namely, accurate, consistent sample preparation [6][7][8].…”

Physicochemical characterisation and α‐amylase inhibitory activity of tea polysaccharides under simulated salivary, gastric and intestinal conditions

“…Fourier transform infrared measurement was used to provide approximate identification about the nature of the TPS before and after simulated digestion in vitro . As shown in Figure S2, the relative peaks within the range of 3600–3200 cm −1 , 3000–2800 cm −1 , 2500–2200 cm −1 , 1800–1000 cm −1 and 800–500 cm −1 were the characteristic absorption peaks of TPS (Wang et al ., ). After simulated digestion, there were no changes on the characteristic peaks of TPS1, TPS2 and TPS3 when compared with those of the original TPS.…”

Fingerprint profiling of polysaccharides from different parts of lotus root varieties

“…All the UV spectra (not shown) of LRPs exhibited strong absorption in the wavelength range of 200-220 nm, assigned to unsaturated carbonyl and carboxyl groups. 11,14 The weak absorption at about 280 nm was primarily attributed to proteins. The spectra of LRPs from different parts showed signicant differences.…”

“…3,9,10 Quality control is considered to be the main issue in the commercialization process of functional polysaccharide products. 11 So far, little is known about the quality control of LRPs.…”

“…A comprehensive understanding of plant polysaccharides' physicochemical similarity and differences among different varieties, locations or tissues is needed for their commercial development. 11,14,15 Varietal and tissue variations in plants are known to impact physicochemical properties of their polysaccharides and consequently the nature and intensity of their bioactivities. 16,17 Previous work indicated that the in vitro antioxidant activities of polysaccharides from the peel and node of lotus root were signicantly stronger than those from the esh.…”