Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A

Abstract

Hghly stable, reusable, and multi-functional biocatalytic microparticles with Laccase (Lac) enzyme (Lac/particles) were synthesized for bisphenol A (BPA) removal from aqueous solution. The Lac/particles were prepared by encapsulating Lac enzymes into poly ethylene glycol (PEG) hydrogel via the UV assisted emulsion polymerization method followed by cross linking with glutaraldehyde (GA). The obtained Lac/particles were spherical and micron sized (137–535 μm), presenting high enzyme entrapment efficiency of 100%, high activity recovery of 18.9%, and great stability at various pHs (3–7) than the free Lac. The Lac/particles could adsorb the BPA into the catalytic particles in a short time, promoting contact between BPA and enzyme, and further enzymatically degrade them without the shaking process and independent surrounding buffer solution. The Lac/particles could be reused for another round BPA adsorption and biotranformation by maintaining over 90% of BPA removal efficiency after seven times reuse. The synergistic effects of adsorption and biocatalytical reaction of Lac/particles have significant values in high efficient and cost-effective BPA removal.

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

“…Laccase (benzenediol: oxygen oxidoreductase, EC 1.10.3.2), belonging to a group of enzymes called multicopper blue oxidasehas been noted to exhibit a wide substrate specificity [1]. It has been applied in various sectors, such as biomedical [2], dye degradation [3], paper industries for delignification [4][5], bioremediation [6], in biosensors [7], as melanin degraders in the cosmetic industry [8], as an enzymatic biofuel [9] and used in juice clarification [10]. Furthermore, laccase is a key biological mediator and the best alternative for chemical mediators; thus, it is regarded as a green enzyme in dye degradation, which is a new era for dye degradation [11].…”

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

“…The physical parameters viz. pH (2)(3)(4)(5)(6)(7)(8)(9)(10), temperature (20- were optimized for optimum production of laccase [30].…”

“…Laccase (benzenediol: oxygen oxidoreductase, EC 1.10.3.2) belonging to a group of an enzyme called a multicopper blue oxidase (MCBO) exhibits wide substrate specificity ranging from phenolic to non-phenolic compounds [1]. It finds applications in various sectors such as biomedical [2], dye degradation [3] (paper industries for delignification [4][5], bioremediation [6], in biosensors [7] in cosmetic industry as melanin degraders [8] and as a enzymatic biofuel [9]. It is the major ligninolytic enzyme used in juice clarification [10].…”

Immobilization of laccase on magnetically separable biochar for highly efficient removal of bisphenol A in water

“…12 Various methods of laccase immobilization on nanomaterials, membranes, and bers [13][14][15] have been accessed by either adsorption, encapsulation, or covalent binding. 12,16,17 However, the development of low cost and environmentally friendly nanomaterials based on stable nanobiocatalysts for the efficient removal of pollutants is still needed.…”