Multi-hierarchical tissue-engineering ECM-like scaffolds based on cellulose acetate with collagen and chitosan fillers

Луканина, К. И.; Grigoriev, T. E.; Krasheninnikov, Sergey V.; Mamagulashvilli, V. G.; Kamyshinsky, Roman; Чвалун, С. Н.

doi:10.1016/j.carbpol.2018.02.061

Cited by 37 publications

(25 citation statements)

References 29 publications

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“…Impregnation solutions were prepared from type II collagen (from rat tails, Belkozin, Luga, Russia) with concentrations of 0.1, 0.5, 1.0% w/w in 2.0% acetic acid/H 2 O. The methodology for diaphragm preparation is described elsewhere . The maximum concentration of filler solution was determined by its ability to diffuse into the dry decellularized diaphragm.…”

Section: Methodsmentioning

confidence: 99%

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Noninvasive ultrasound imaging for assessment of intact microstructure of extracellular matrix in tissue engineering

et al. 2019

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Development of artificial tissues or organs is one of the actual tasks in regenerative medicine that requires observation and evaluation of intact volume microstructure of tissue engineering products at all stages of their formation, from native donor tissues and decellularized scaffolds to recipient cell migration in the matrix. Unfortunately in practice, methods of vital noninvasive imaging of volume microstructure in matrixes are absent. In this work, we propose a new approach based on high‐frequency acoustic microscopy for noninvasive evaluation and visualization of volume microstructure in tissue engineering products. The results present the ultrasound characterization of native rat diaphragms and lungs and their decellularized scaffolds. Verification of the method for visualization of tissue formation in the matrix volume was described in the model samples of diaphragm scaffolds with stepwise collagenization. Results demonstrate acoustic microscopic sensitivity to cell content concentration, variation in local density, and orientation of protein fibers in the volume, micron air inclusions, and other inhomogeneities of matrixes.

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Section: Methodsmentioning

confidence: 99%

“…The methodology for diaphragm preparation is described elsewhere. 22 The maximum concentration of filler solution was determined by its ability to diffuse into the dry decellularized diaphragm. The polymer filler mass in composite is proportional to the concentration of collagen solution and varies from 0% to 15%.…”

Section: Collagenizationmentioning

confidence: 99%

Noninvasive ultrasound imaging for assessment of intact microstructure of extracellular matrix in tissue engineering

et al. 2019

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“…K.I. Lukanina et al, on its turn, generated a sponge-nonwoven CA matrix by filling the electrospun mat with chitosan and collagen and posteriorly freezing and vacuum drying the combination [69]. The natural, microbial, and biodegradable thermoplastic polymer poly(hydroxybutyrate) (PHB) was blended with CA and the processed by electrospinning to generate fibrous nanocomposites with a considerable capacity to induce cell adhesion and proliferation.…”

Section: Nanostructured Wound Dressingsmentioning

confidence: 99%

Electrospun Nanocomposites Containing Cellulose and Its Derivatives Modified with Specialized Biomolecules for an Enhanced Wound Healing

et al. 2020

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Wound healing requires careful, directed, and effective therapies to prevent infections and accelerate tissue regeneration. In light of these demands, active biomolecules with antibacterial properties and/or healing capacities have been functionalized onto nanostructured polymeric dressings and their synergistic effect examined. In this work, various antibiotics, nanoparticles, and natural extract-derived products that were used in association with electrospun nanocomposites containing cellulose, cellulose acetate and different types of nanocellulose (cellulose nanocrystals, cellulose nanofibrils, and bacterial cellulose) have been reviewed. Renewable, natural-origin compounds are gaining more relevance each day as potential alternatives to synthetic materials, since the former undesirable footprints in biomedicine, the environment, and the ecosystems are reaching concerning levels. Therefore, cellulose and its derivatives have been the object of numerous biomedical studies, in which their biocompatibility, biodegradability, and, most importantly, sustainability and abundance, have been determinant. A complete overview of the recently produced cellulose-containing nanofibrous meshes for wound healing applications was provided. Moreover, the current challenges that are faced by cellulose acetate- and nanocellulose-containing wound dressing formulations, processed by electrospinning, were also enumerated.

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“…A highly porous structure is formed during solvent sublimation from a frozen polymer solution in high vacuum. The suggested method combine was shown to be a promising way of biomaterial developing 10,11 . Depending on the intended use the scaffold should provide the appropriate morphology and mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

“…In the previous work, fibrillar collagen and semi‐crystalline chitosan have been demonstrated to be structured differently and noticeably affect the mechanical properties of composites with the hydrophilic matrix and fillers 10 . Hence, in the experiments with hydrophobic PLLA matrix and hydrophilic fillers, collagen, and chitosan were chosen to observe the influence of polymer nature on the composite properties.…”

Section: Introductionmentioning

confidence: 99%

Study of highly porous poly‐l‐lactide‐based composites with chitosan and collagen

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Луканина

Krasheninnikov

et al. 2020

Polymers for Advanced Techs

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Increased interest in the field of tissue engineering promotes the development of biomaterial design. Aspiration to reconstruct native extracellular matrix architecture (ECM) expands the number of applying approaches in polymer scaffold development. In the present work, we proposed a combination of electrospinning and lyophilization techniques to produce materials with ECM‐like morphology based on poly‐l‐lactide fiber matrix with natural bio‐adequate fillers (collagen and chitosan). Morphology, mechanical properties, and hydrophilicity of the developed biomaterials were studied. The introduction of the collagen filler enhanced the strength and Young's modulus of the composites by 190% and 80%. The same characteristics of the chitosan‐containing material were increased by 430% and 340%, respectively. It exceeded the collagen ones, which was caused by the better chitosan penetration into the fiber matrix and, as a result, the percolation network formation. The possibility of material wettability improving was shown.

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Multi-hierarchical tissue-engineering ECM-like scaffolds based on cellulose acetate with collagen and chitosan fillers

Cited by 37 publications

References 29 publications

Noninvasive ultrasound imaging for assessment of intact microstructure of extracellular matrix in tissue engineering

Noninvasive ultrasound imaging for assessment of intact microstructure of extracellular matrix in tissue engineering

Electrospun Nanocomposites Containing Cellulose and Its Derivatives Modified with Specialized Biomolecules for an Enhanced Wound Healing

Study of highly porous poly‐l‐lactide‐based composites with chitosan and collagen

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