Multi-<i>Omics</i> Data Mining: A Novel Tool for BioBrick Design

Burgos-Toro, Angie; Dippe, Martin; Vásquez, Andrés Felipe; Pierschel, Eric; Wessjohann, Ludger A.; Fernández-Niño, Miguel

doi:10.5772/intechopen.101351

Cited by 2 publications

(2 citation statements)

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“…In general, these issues can be avoided using computational strategies to overcome challenges associated with the high cost of production, the potency of AMPs, and reduce the rate of resistance, degradation, toxicity, and instability (Cardoso et al, 2020 ; Dijksteel et al, 2021 ). Another option is the use of multi-omics (including genomics, transcriptomics, and proteomics) which allows identifying a novel sequence of AMPs (Chen et al, 2019 ; Burgos-Toro et al, 2021 ).…”

Section: Antimicrobial Peptides As An Alternative For Livestock Treat...mentioning

confidence: 99%

Antimicrobial Peptides Controlling Resistant Bacteria in Animal Production

2022

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In the last few decades, antimicrobial resistance (AMR) has been a worldwide concern. The excessive use of antibiotics affects animal and human health. In the last few years, livestock production has used antibiotics as food supplementation. This massive use can be considered a principal factor in the accelerated development of genetic modifications in bacteria. These modifications are responsible for AMR and can be widespread to pathogenic and commensal bacteria. In addition, these antibiotic residues can be dispersed by water and sewer water systems, the contamination of soil and, water and plants, in addition, can be stocked in tissues such as muscle, milk, eggs, fat, and others. These residues can be spread to humans by the consumption of water or contaminated food. In addition, studies have demonstrated that antimicrobial resistance may be developed by vertical and horizontal gene transfer, producing a risk to public health. Hence, the World Health Organization in 2000 forbid the use of antibiotics for feed supplementation in livestock. In this context, to obtain safe food production, one of the potential substitutes for traditional antibiotics is the use of antimicrobial peptides (AMPs). In general, AMPs present anti-infective activity, and in some cases immune response. A limited number of AMP-based drugs are now available for use in animals and humans. This use is still not widespread due to a few problems like in-vivo effectiveness, stability, and high cost of production. This review will elucidate the different AMPs applications in animal diets, in an effort to generate safe food and control AMR.

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Section: Antimicrobial Peptides As An Alternative For Livestock Treat...mentioning

confidence: 99%

Antimicrobial Peptides Controlling Resistant Bacteria in Animal Production

2022

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“…Coupling these differential metabolite profile data with gene transcript levels can assist in probing involved genes, biosynthetic pathway analyses, and eventually breeding if targeting increasing levels of that phytochemical. 74,75 Indeed, the correlative analysis of differential metabolic and gene expression profiling in planta has proven a powerful approach for the identification of candidate genes and enzymes, particularly for those of natural product metabolism. This aids the identification of the best times for transcriptome analyses and for harvesting certain phytochemicals or enzymes.…”

Section: Metabolite Quantification In Organsmentioning

confidence: 99%

A multiplex approach of MS, 1D‐, and 2D‐NMR metabolomics in plant ontogeny: A case study on Clusia minor L. organs (leaf, flower, fruit, and seed)

Noleto‐Dias,

Farag,

Porzel

et al. 2023

Phytochemical Analysis

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IntroductionThe genus Clusia L. is mostly recognised for the production of prenylated benzophenones and tocotrienol derivatives.ObjectivesThe objective of this study was to map metabolome variation within Clusia minor organs at different developmental stages.Material and MethodsIn total 15 organs/stages (leaf, flower, fruit, and seed) were analysed by UPLC‐MS and 1H‐ and heteronuclear multiple‐bond correlation (HMBC)‐NMR‐based metabolomics.ResultsThis work led to the assignment of 46 metabolites, belonging to organic acids(1), sugars(2) phenolic acids(1), flavonoids(3) prenylated xanthones(1) benzophenones(4) and tocotrienols(2). Multivariate data analyses explained the variability and classification of samples, highlighting chemical markers that discriminate each organ/stage. Leaves were found to be rich in 5‐hydroxy‐8‐methyltocotrienol (8.5 μg/mg f.w.), while flowers were abundant in the polyprenylated benzophenone nemorosone with maximum level detected in the fully mature flower bud (43 μg/mg f.w.). Nemorosone and 5‐hydroxy tocotrienoloic acid were isolated from FL6 for full structural characterisation. This is the first report of the NMR assignments of 5‐hydroxy tocotrienoloic acid, and its maximum level was detected in the mature fruit at 50 μg/mg f.w. Seeds as typical storage organ were rich in sugars and omega‐6 fatty acids.ConclusionTo the best of our knowledge, this is the first report on a comparative 1D‐/2D‐NMR approach to assess compositional differences in ontogeny studies compared with LC‐MS exemplified by Clusia organs. Results derived from this study provide better understanding of the stages at which maximal production of natural compounds occur and elucidate in which developmental stages the enzymes responsible for the production of such metabolites are preferentially expressed.

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Multi-Omics Data Mining: A Novel Tool for BioBrick Design

Cited by 2 publications

References 153 publications

Antimicrobial Peptides Controlling Resistant Bacteria in Animal Production

Antimicrobial Peptides Controlling Resistant Bacteria in Animal Production

A multiplex approach of MS, 1D‐, and 2D‐NMR metabolomics in plant ontogeny: A case study on Clusia minor L. organs (leaf, flower, fruit, and seed)

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