Multi-level toxicity assessment of engineered cellulose nanofibrils in<i>Daphnia magna</i>

Ogonowski, Martin; Edlund, Ulrica; Gorokhova, Elena; Linde, Margareta; Ek, Karin; Liewenborg, Birgitta; O, Könnecke; Navarro, Julien R. G.; Breitholtz, Magnus

doi:10.1080/17435390.2018.1464229

Cited by 30 publications

(10 citation statements)

References 57 publications

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“…20 Contrariwise, Daphnia magna treated with CSC nanofibrils (0.206–20.6 mg L −1 ) showed no clinical signs of toxicity (no mortality, no apparent translocation of CSC nanofibrils across the alimentary canal, and no aberrant behavior) after 21 days of treatment. 21 Also, another study showed low toxicity potential of surface-modified CSC-nanocrystals and CSC-nanofibers in embryonic zebra fish. 22 We had also previously reported that oral administration of sulfonated CSN could cause mild toxicity in rat renal tissues.…”

Section: Introductionmentioning

confidence: 99%

In vivo hepatotoxicity of chemically modified nanocellulose in rats

et al. 2019

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Chemical modification of cellulose is currently attracting attention as researchers attempt to take advantage of the abundance of hydroxyl groups on its surface to introduce extra biological functionality. However, the possible deleterious effect of exposure to functionalized nanocellulose (CSN) remains a concern. Therefore, this study aims to explore the potential mechanisms of hepatotoxicity of CSN modified with oxalate ester (NCD) in rats. A 7-day repeated oral toxicity study of NCD at the doses of 50 and 100 mg kg−1 body weight was conducted, and plasma and liver tissue samples were assayed using biochemical analysis, liver histopathology, and protein expression. NCD, at both doses, did not significantly ( p > 0.05) alter the relative weight of liver, alkaline phosphatase activity, and lipid peroxidation levels of the animals. However, NCD at the dose of 100 mg kg−1 body weight significantly elevated aspartate aminotransferase, alanine aminotransferase, and myeloperoxidase activities. NCD also enhanced the immunohistochemical expression of inducible nitric oxide synthase and Bcl-2-associated X protein in the liver of rats. Histological observations revealed necrosis and severe cellular infiltration at the high-dose treatment. Our study provides an experimental basis for the safe application of NCDs.

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Section: Introductionmentioning

confidence: 99%

In vivo hepatotoxicity of chemically modified nanocellulose in rats

et al. 2019

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“…3A. Ogonowski et al (2018) have examined the response of D. magna neonates after 48 h of exposure to CNFs and also observed no mortality.…”

Section: Nanocelluloses Induced Toxicity and Oxidative Stress Responsmentioning

confidence: 99%

“…Vartiainen et al (2011) detected acute toxicity of microfibrillated cellulose referred to as nanocellulose only at a very high level (300 mg/L) to Vibrio fischeri, and detected decrease in Daphnia magna movement induced by the microfibrillated cellulose. Ogonowski et al (2018) also concluded that CNFs display a low toxic potential to filter-feeding organism (D. magna). In addition, Salehpour et al (2018) found that the nanocomposites of polyvinyl alcohol and CNFs did not generate any negative effects on plants including cress and spinach.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress actuated by cellulose nanocrystals and nanofibrils in aquatic organisms of different trophic levels

Wang

Song

et al. 2020

NanoImpact

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Nanocellulose is a functional material derived from natural carbon-based polymers. These nanomaterials are biodegradable and renewable in nature and hence are seen as environmentally-friendly materials in many applications. The use of such innovative materials is accelerating and inescapable there is a need to test these presumed environmentally-friendly materials with regard to their ecotoxicity. Here, the acute toxicity and the oxidative stress of nanocelluloses as induced to three aquatic organisms of different trophic levels, namely Scenedesmus obliquus, Daphnia magna, and Danio rerio, were studied in relation to the composition and morphology of the celluloses. Wood-based cellulose nanocrystals (CNCs), cotton-based CNCs, and cotton-based cellulose nanofibrils were selected as model compounds. The results clearly demonstrated a lack of impact of the different nanocellulose materials on apical endpoints like growth inhibition and mortality after short-term exposure. The nanocellulose materials did activate oxidative stress as evoked by reactive oxygen species in the three aquatic organisms. Key factors ascertained to induce the oxidative stress were the composition and morphology. The nanocellulose induced oxidative stress was observed for all the species at concentrations higher than 0.01 mg/L. This finding suggests a more general revelation of oxidative stress being a characteristic mechanism for nanocellulose toxicity to aquatic organisms.

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“…CNF further caused peritoneal inflammation [19]. In Daphnia magna CNF displayed a low-toxic potential, indicating a low environmental risk factor [22]. The current study used the Drosophila model to check the toxicity of CNF.…”

Section: Introductionmentioning

confidence: 99%

Wood-Based Cellulose Nanofibrils: Haemocompatibility and Impact on the Development and Behaviour of Drosophila melanogaster

et al. 2019

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Wood-based cellulose nanofibrils (CNF) offer an excellent scaffold for drug-delivery formulation development. However, toxicity and haemocompatibility of the drug carrier is always an important issue. In this study, toxicity-related issues of CNF were addressed. Different doses of CNF were orally administered to Drosophila and different tests like the developmental cycle, trypan blue exclusion assay, larva crawling assay, thermal sensitivity assay, cold sensitivity assay, larval light preference test, climbing behaviour, nitroblue tetrazolium (NBT) reduction assay, adult phenotype, and adult weight were conducted to observe the impact on its development and behaviour. A haemocompatibility assay was done on the blood taken from healthy Wistar rats. In Drosophila, the abnormalities in larval development and behaviour were observed in the behavioural assays. However, the cytotoxic effect could not be confirmed by the gut staining and level of reactive oxygen species. The larvae developed into an adult without any abnormality in the phenotype. The CNF did cause loss of weight in the adult flies and did not cause much toxicity within the body since there was no phenotypic defect. Hemolysis data also suggested that CNF was safe at lower doses, as the data was well within acceptable limits. All these results suggest that cellulose nanofibres have no significant cytotoxic effects on Drosophila. However, the developmental and behavioural abnormalities suggest that CNF may act as a behavioural teratogen.

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Multi-level toxicity assessment of engineered cellulose nanofibrils inDaphnia magna

Cited by 30 publications

References 57 publications

In vivo hepatotoxicity of chemically modified nanocellulose in rats

In vivo hepatotoxicity of chemically modified nanocellulose in rats

Oxidative stress actuated by cellulose nanocrystals and nanofibrils in aquatic organisms of different trophic levels

Wood-Based Cellulose Nanofibrils: Haemocompatibility and Impact on the Development and Behaviour of Drosophila melanogaster

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