Multi‐locus sequencing reveals a novel
            <i>Bartonella</i>
            in mammals from the Superorder Xenarthra

Calchi, Ana Cláudia; Vultão, Juliana Gaboardi; Alves, Mário Henrique; Yogui, Débora Regina; Desbiez, Arnaud Léonard Jean; Amaral, Renan Bressianini do; Santi, Mariele de; Teixeira, Marta Maria Geraldes; Werther, Karin; Machado, Rosangela Zacarias; André, Marcos Rogério

doi:10.1111/tbed.13545

Cited by 18 publications

(6 citation statements)

References 54 publications

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“…There are many Bartonella genotypes described in Brazilian mammals, primarily rodents and bats ( André et al, 2019b ; do Amaral et al, 2018 ; de Sousa et al, 2018 ; Ferreira et al, 2018 ; Rozental et al, 2017 ; Ikeda et al, 2017 ; Gonçalves et al, 2016a ). In the present study, we analyzed not only the Bartonella genotypes found in the rodents and bats specimens collected, but also included the data from the other genotypes described in these and in other mammals from wild and domestic environments in Brazil ( André et al, 2014 , 2019a ; Gonçalves et al, 2020 ; Pedrassani et al, 2019 ; Silva et al, 2019 ; Calchi et al, 2020 ; Miceli et al, 2013 ; Filoni et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

“…Tokpelaia hoelldoblerii ( Segers et al, 2017 ) were used as the outgroups. The final alignments of the gltA (718 bps), ftsZ (788 bps), and groEL (798 bps), and the Brazilian genotypes of each gene were included in the analyses ( Gonçalves et al, 2020 ; Calchi et al, 2020 ; André et al, 2019a ; André et al, 2019b ; Pedrassani et al, 2019 ; Silva et al, 2019 ; do Amaral et al, 2018 ; de Sousa et al, 2018 ; Ferreira et al, 2018 ; Rozental et al, 2017 ; Ikeda et al, 2017 ; Gonçalves et al, 2016a ; Diniz et al, 2016 ; André et al, 2014 ; Miceli et al, 2013 ; Filoni et al, 2012 ). All the sequences were aligned using the MAFFT v7.388 algorithm for each sequenced gene ( Katoh and Standley, 2013 ).…”

Section: Methodsmentioning

confidence: 99%

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Investigation of Bartonella spp. in brazilian mammals with emphasis on rodents and bats from the Atlantic Forest

Gonçalves-Oliveira

Rozental

Guterres

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

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The Bartonella species are zoonotic agents that infect mammals and are transmitted by arthropod vectors. Approximately 18 distinct genotypes cause diseases in humans, and may be spread by both domestic and wild animals. In Brazil, Bartonella genotypes have been identified in several species of wild mammals, and in the present study, we analyzed samples from non-human primates (marmosets), marsupials, rodents, and bats, and compared them with the genotypes described in mammals from Brazil, to examine the distribution of Bartonella genotypes in two impacted areas of Rio de Janeiro state, in southeastern Brazil. We used polymerase chain reaction (PCR) methods to detect the Bartonella DNA using partial sequences of the gltA, ftsZ , and groEL genes. We generated Bayesian inference and maximum likelihood trees to characterize the positive PCR samples and infer the phylogenetic relationships of the genotypes. A total of 276 animals were captured, including 110 bats, 91 rodents, 38 marsupials, and 37 marmosets. The DNA of Bartonella was amplified from tissue samples collected from 12 (4.34%) of the animals, including eight rodents – Akodon cursor (5/44) and Nectomys squamipes (3/27) – and four bats, Artibeus lituratus (3/58) and Carollia perspicillata (1/15). We identified Bartonella genotypes closely related to those described in previous studies, as well as new genotypes in both the rodent and the bat samples. Considering the high diversity of the Bartonella genotypes and hosts identified in the present study, further research is needed to better understand the relationships between the different Bartonella genotypes and their vectors and host species. The presence of Bartonella in the wild rodents and bats from the study area indicates that the local human populations may be at risk of infection by Bartonella due to the spillover of these strains from the wild environment to domestic and peri-domestic environments.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Investigation of Bartonella spp. in brazilian mammals with emphasis on rodents and bats from the Atlantic Forest

Gonçalves-Oliveira

Rozental

Guterres

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

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“…More recently, a new Bartonella Candidatus ("Candidatus Bartonella washoensis subspecies brasiliensis"), two new Anaplasma Candidatus ("Candidatus Anaplasma brasiliensis" and "Candidatus Anaplasma amazonensis") and two Ehrlichia species (Ehrlichia canis and Ehrlichia minasensis) have been described in free-living Xenarthra from Brazil (Calchi, Vultão, Alves, Yogui, Desbiez, Amaral, et al, 2020;.…”

Section: Introductionmentioning

confidence: 99%

“…genotype related to Ehrlichia ruminantium was detected in a three‐toed sloth ( Bradypus tridactylus ) in Pará state, northern Brazil (Soares et al., 2017). More recently, a new Bartonella Candidatus (“ Candidatus Bartonella washoensis subspecies brasiliensis”), two new Anaplasma Candidatus (“ Candidatus Anaplasma brasiliensis” and “ Candidatus Anaplasma amazonensis”) and two Ehrlichia species ( Ehrlichia canis and Ehrlichia minasensis ) have been described in free‐living Xenarthra from Brazil (Calchi, Vultão, Alves, Yogui, Desbiez, Amaral, et al., 2020; Calchi, Vultão, Alves, Yogui, Desbiez, Santi, et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

Molecular investigation of haemotropic mycoplasmas and Coxiella burnetii in free‐living Xenarthra mammals from Brazil, with evidence of new haemoplasma species

Oliveira

Calchi

Vultão

et al. 2022

Transbounding Emerging Dis

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Although mammals of the superorder Xenarthra are considered hosts of a wide range of zoonotic agents, works aiming at investigating the role of these animals as hosts for bacteria with zoonotic potential are rare. The present study aimed to investigate the occurrence and molecularly characterize Coxiella burnetii and haemoplasma (haemotropic mycoplasmas) DNA in blood and spleen samples from 397 free-living Xenarthra mammals (233 sloths, 107 anteaters and 57 armadillos) in five Brazilian states (Mato Grosso do Sul, São Paulo, Pará, Rondônia and Rio Grande do Sul). All biological samples from Xenarthra were negative in the qPCR for Coxiella burnetii based on the IS1111 gene. The absence of C. burnetii DNA in blood and spleen samples from Xenarthra suggests that these mammals may not act as possible hosts for this agent in the locations studied. When performed conventional PCR assays for the endogenous (gapdh) mammalian gene, 386 samples were positive. When screened by molecular assays based on the 16S rRNA gene of haemoplasmas, 81 samples were positive, of which 15.54% (60/386) were positive by conventional PCR and 5.44% (21/386) were positive by real-time PCR; three samples were positive in both assays.Of these, 39.74% (31/78) were also positive for the 23S rRNA gene and 7.69% (6/78) for the haemoplasma RNAse P gene. Among the samples positive for haemoplasmas, 25.64% (20/78) were obtained from anteaters (Tamandua tetradactyla and Myrmecophaga tridactyla), 39.74% (31/78) from sloths (Bradypus tridactylus, Bradypus sp. and Choloepus sp.) 34.61% (27/78) from armadillos (Priodontes maximus, Euphractus sexcinctus and Dasypus novemcinctus). A haemoplasma 16S rRNA sequence closely related and showing high identity (99.7%) to Mycoplasma wenyonii was detected, for the first time, in B. tridactylus. Based on the low identity and phylogenetic positioning of 16S rRNA and 23S rRNA sequences of haemoplasmas detected in anteaters and armadillos, the present study showed, for the first time, the occurrence of putative new

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“…Multilocus sequence typing (MLST) studies have been conducted on Bartonella spp. contributing to clarifying phylogenetic relationships, host specificity, diversity and the discovery of novel species [ 21 , 27 , 28 , 29 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal

et al. 2022

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Bartonella spp. comprises emergent and re-emergent fastidious Gram-negative bacteria with worldwide distribution. Cats are the main reservoir hosts for Bartonella henselae and dogs represent opportunistic hosts for the bacteria. Even though ticks may also play a role in transmission, their competence as vectors for Bartonella spp. has not been totally understood. Considering only a few studies had a focus on screening Bartonella in animals, humans and ectoparasites in Portugal, this study aimed to address the molecular occurrence of Bartonella sp. in 123 stray cats, 25 stray dogs, 30 humans from Lisbon and 236 questing ticks within the country. Using a qPCR targeting the nuoG gene, it was possible to detect Bartonella sp. DNA on 20.32% of cat samples (25/123). From these positive samples, 13 sequences were characterized as B. henselae, 11 as B. clarridgeiae and 1 presented co-infection with both species. The absolute quantification of nuoGBartonella DNA in sampled cats ranged from 2.78 × 10 to 1.03 × 105 copies/µL. The sampled dogs, humans and ticks were negative. These results showed that B. henselae and B. clarridgeiae are circulating in stray cats from Lisbon. Additional and more extended studies should be conducted to determine the impact of such infections on humans, particularly those in constant and direct contact with cats.

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Multi‐locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra

Cited by 18 publications

References 54 publications

Investigation of Bartonella spp. in brazilian mammals with emphasis on rodents and bats from the Atlantic Forest

Investigation of Bartonella spp. in brazilian mammals with emphasis on rodents and bats from the Atlantic Forest

Molecular investigation of haemotropic mycoplasmas and Coxiella burnetii in free‐living Xenarthra mammals from Brazil, with evidence of new haemoplasma species

Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal

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