Multi metabolomics-based analysis of application of Astragalus membranaceus in the treatment of hyperuricemia

Abstract

Hyperuricemia (HUA) is a common metabolic disease that is an independent risk factor for comorbidities such as hypertension, chronic kidney disease, and coronary artery disease. The prevalence of HUA has increased over the last several decades with improved living standards and increased lifespans. Metabolites are considered the most direct reflection of individual physiological and pathological conditions, and represent attractive candidates to provide deep insights into disease phenotypes. Metabolomics, a technique used to profile metabolites in biofluids and tissues, is a powerful tool for identification of novel biomarkers, and can be used to provide valuable insights into the etiopathogenesis of metabolic diseases and to evaluate the efficacy of drugs. In this study, multi metabolomics-based analysis of the blood, urine, and feces of rats with HUA showed that HUA significantly altered metabolite profiles. Astragalus membranaceus (AM) and benbromomalone significantly mitigated these changes in blood and feces, but not in urine. Some crucial metabolic pathways including lipid metabolism, lipid signaling, hormones synthesis, unsaturated fatty acid (UFAs) absorption, and tryptophan metabolism, were seriously disrupted in HUA rats. In addition, AM administration exerted better treatment effects on HUA than benbromomalone. Furthermore, additional supplementation with UFAs and tryptophan may also induce therapeutic effects against HUA.

Identification of Hyperuricemia Alleviating Peptides from Yellow Tuna Thunnus albacares

“…Compared with the MC group, it was found that 196 serum metabolites were significantly upregulated and 362 metabolites were significantly downregulated in the TAP group in positive mode. Meanwhile, it was found that 210 serum metabolites were significantly upregulated and 355 metabolites were significantly downregulated in positive mode, suggesting that TAP altered the metabolite profiles of PO-induced HUA, similar to the effect of Astragalus membranaceus …”

“…Meanwhile, it was found that 210 serum metabolites were significantly upregulated and 355 metabolites were significantly downregulated in positive mode, suggesting that TAP altered the metabolite profiles of POinduced HUA, similar to the effect of Astragalus membranaceus. 49 Variable importance for the projection (VIP) is used to measure the expression patterns of various metabolites and explore biologically significant differential metabolites. The screening criteria for significantly different metabolites in this paper are VIP >1 and a P value < 0.05.…”

The Therapeutic Effect and the Potential Mechanism of Flavonoids and Phenolics of Moringa oleifera Lam. Leaves against Hyperuricemia Mice

“…Principal component analysis (PCA) is a representative of unsupervised algorithms, which can reflect the spatial distribution of the original data of the samples without any artificial factor grouping. The closer the spatial distribution of the samples to each other, the closer the composition of the metabolite components within the samples [ 22 , 23 , 24 ]. In the current research, PCA was carried out to discern the differences of metabolite fingerprints in serum among NG, MG, and DG.…”

16S rRNA and Metagenomics Combined with UPLC-Q/TOF-MS Metabolomics Analysis Reveals the Potential Mechanism of Radix Astragali Against Hyperuricemia in Mice

“…More excitingly, many studies have reported that RA can treat hyperuricemia. 15 , 16 However, RA is composed mainly of flavonoids, saponins and polysaccharides, which are difficult to absorb directly in the digestive tract. 17 , 18 Therefore, it is important to understand the mechanism of RA in improving hyperuricemia.…”