Multi-modal imaging probe for assessing the efficiency of stem cell delivery to orthotopic breast tumours

Thin, May Zaw; Allan, H. R.; Bofinger, Robin; Kostelec, Tomas D.; Simon, Guillaume; Connell, John; Patrick, P. Stephen; Hailes, Helen C.; Tabor, Alethea B.; Lythgoe, Mark F.; Stuckey, Daniel J.; Kalber, Tammy L.

doi:10.1039/d0nr03237a

Cited by 17 publications

(24 citation statements)

References 60 publications

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“…729 Hence these mechanisms can be taken advantage of to enable the radiolabelling of cells, to facilitate in vivo cell tracking. A variety of different cell types have been radiolabelled with chitosan-based NPs, 503,504 gold NPs, 730,731 SPIONs, 213,610,732 and mesoporous silica NPs. 721,733 The efficiency of this cell labelling process is exemplified by Jung et al who used 68 Ga-labelled MSNPs to radiolabel breast cancer cells with enough activity (ca.…”

Section: Cell Trackingmentioning

confidence: 99%

Radiolabelling of nanomaterials for medical imaging and therapy

2021

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Section: Cell Trackingmentioning

confidence: 99%

Radiolabelling of nanomaterials for medical imaging and therapy

2021

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“…However, photoacoustic imaging suffers from operator dependent variability [ 255 ]. More recently, multimodal imaging probes that combine the advantage of different imaging modalities have been developed, including PET/MRI imaging agent [ 256 ], SPECT/MRI/fluorescent imaging agent [ 257 ], and SPECT/MRI/BLI imaging agent [ 258 ].…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal stem/stromal cell-based therapy: mechanism, systemic safety and biodistribution for precision clinical applications

et al. 2021

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Mesenchymal stem/stromal cells (MSCs) are a promising resource for cell-based therapy because of their high immunomodulation ability, tropism towards inflamed and injured tissues, and their easy access and isolation. Currently, there are more than 1200 registered MSC clinical trials globally. However, a lack of standardized methods to characterize cell safety, efficacy, and biodistribution dramatically hinders the progress of MSC utility in clinical practice. In this review, we summarize the current state of MSC-based cell therapy, focusing on the systemic safety and biodistribution of MSCs. MSC-associated risks of tumor initiation and promotion and the underlying mechanisms of these risks are discussed. In addition, MSC biodistribution methodology and the pharmacokinetics and pharmacodynamics of cell therapies are addressed. Better understanding of the systemic safety and biodistribution of MSCs will facilitate future clinical applications of precision medicine using stem cells.

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“…This first BLI signal detection can be influenced from the number of cells transplanted to model used as a receptor [63,67]. Therefore, the concomitant evaluation using NIRF in the acute evaluation and BLI to follow up for a long time showed the importance of the complementary use of two or more techniques to evaluate the biological process in vivo in a non-invasive way [68]. Due to magnetic feature of SPION NIRF-Rh could also have been carried out the MRI evaluation, [25][26][27] but as we did not have this equipment for animal scanner images, the evaluation was not carried out.…”

Section: Discussionmentioning

confidence: 99%

Multimodal Tracking of Hematopoietic Stem Cells from Young and Old Mice Labeled with Magnetic–Fluorescent Nanoparticles and Their Grafting by Bioluminescence in a Bone Marrow Transplant Model

et al. 2021

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This study proposes an innovative way to evaluate the homing and tracking of hematopoietic stem cells from young and old mice labeled with SPIONNIRF-Rh conjugated with two types of fluorophores (NIRF and Rhodamine), and their grafting by bioluminescence (BLI) in a bone marrow transplant (BMT) model. In an in vitro study, we isolated bone marrow mononuclear cells (BM-MNC) from young and old mice, and analyzed the physical–chemical characteristics of SPIONNIRF-Rh, their internalization, cell viability, and the iron quantification by NIRF, ICP-MS, and MRI. The in vivo study was performed in a BMT model to evaluate the homing, tracking, and grafting of young and old BM-MNC labeled with SPIONNIRF-Rh by NIRF and BLI, as well as the hematological reconstitution for 120 days. 5FU influenced the number of cells isolated mainly in young cells. SPIONNIRF-Rh had adequate characteristics for efficient internalization into BM-MNC. The iron load quantification by NIRF, ICP-MS, and MRI was in the order of 104 SPIONNIRF-Rh/BM-MNC. In the in vivo study, the acute NIRF evaluation showed higher signal intensity in the spinal cord and abdominal region, and the BLI evaluation allowed follow-up (11–120 days), achieving a peak of intensity at 30 days, which remained stable around 108 photons/s until the end. The hematologic evaluation showed similar behavior until 30 days and the histological results confirm that iron is present in almost all tissue evaluated. Our results on BM-MNC homing and tracking in the BMT model did not show a difference in migration or grafting of cells from young or old mice, with the hemogram analysis trending to differentiation towards the myeloid lineage in mice that received cells from old animals. The cell homing by NIRF and long term cell follow-up by BLI highlighted the relevance of the multimodal nanoparticles and combined techniques for evaluation.

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Multi-modal imaging probe for assessing the efficiency of stem cell delivery to orthotopic breast tumours

Abstract: Illustration of adipose-derived stem cells with tri-modal imaging capabilities for evaluating the efficiency of cell delivery to tumours.

Cited by 17 publications

References 60 publications

Radiolabelling of nanomaterials for medical imaging and therapy

Radiolabelling of nanomaterials for medical imaging and therapy

Mesenchymal stem/stromal cell-based therapy: mechanism, systemic safety and biodistribution for precision clinical applications

Multimodal Tracking of Hematopoietic Stem Cells from Young and Old Mice Labeled with Magnetic–Fluorescent Nanoparticles and Their Grafting by Bioluminescence in a Bone Marrow Transplant Model

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