Multi-mycotoxins Analysis in Dried Fruit by LC/MS/MS and a Modified QuEChERS Procedure

Azaiez, Inés; Giusti, Federica; Sagratini, Gianni; Mañes, Jordi; Fernández-Franzón, Mónica

doi:10.1007/s12161-013-9785-3

Cited by 69 publications

(46 citation statements)

References 27 publications

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“…Methods based on GC usually require a tedious derivatisation step. LC-MS/MS is an important tool for the analysis of various mycotoxins in agriculture products due to its high selectivity and specificity (Warth et al 2012;Al-Taher et al 2013;Azaiez et al 2014;Tsiplakou et al 2014;Ediage et al 2015;Jung et al 2015;Fabregat-Cabello et al 2016). However, a rapid and appropriate clean-up procedure is necessary to remove severe matrix effects in HPLC-MS analysis.…”

Section: Introductionmentioning

confidence: 99%

Quantification of mycotoxins in vegetable oil by UPLC-MS/MS after magnetic solid-phase extraction

Zhao

Wan

Bai

et al. 2017

Food Additives & Contaminants: Part A

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The detection of mycotoxin contamination in foodstuffs is highly significant for public health. Herein we report an analytical method based on magnetic solid-phase extraction (MSPE) and UPLC-MS/MS for the simultaneous determination of mycotoxins, including fumonisins B 1 (FB 1 ), zearalenone (ZON) and ochratoxin A (OTA), in vegetable oil. Magnetic nanoparticles coated with double layers of silicon dioxide were synthesised and found to be an effective MSPE adsorbent for mycotoxins. The proposed MSPE procedure serves not only for sample clean-up but also for mycotoxin enrichment that enhances greatly the assay's sensitivity. Under the selected MSPE conditions, linear matrix-matched calibration curves were obtained for mycotoxins in a concentration range from 0.178 to 625 μg kg -1. The limits of detection were 0.210 μg kg -1 for FB 1 , 0.0800 μg kg -1 for OTA and 1.03 μg kg -1 for ZON. The proposed MSPE UPLC-MS/MS method was applied for the determination of mycotoxins in vegetable oil samples, including maize oil, rapeseed oil and soybean oil. ZON was detected in a maize oil at 101 μg kg -1 , which is below the European Union limit of 200 μg kg -1 in foodstuffs.ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Quantification of mycotoxins in vegetable oil by UPLC-MS/MS after magnetic solid-phase extraction

Zhao

Wan

Bai

et al. 2017

Food Additives & Contaminants: Part A

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show abstract

“…In order to reduce the matrix effects (MEs), which can result in unsatisfactory recoveries, additional purification with sorbents such as octadecyl silica (C18), primary secondary amine (PSA), and graphitized carbon black (GCB) are commonly used. The sorbents react differently depending on the physicochemical properties of the compounds constituting the samples [26,27]. …”

Section: Introductionmentioning

confidence: 99%

QuEChERS Purification Combined with Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry for Simultaneous Quantification of 25 Mycotoxins in Cereals

Sun

Zhang

et al. 2016

Toxins

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A method based on the QuEChERS (quick, easy, cheap, effective, rugged, and safe) purification combined with ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS), was optimized for the simultaneous quantification of 25 mycotoxins in cereals. Samples were extracted with a solution containing 80% acetonitrile and 0.1% formic acid, and purified with QuEChERS before being separated by a C18 column. The mass spectrometry was conducted by using positive electrospray ionization (ESI+) and multiple reaction monitoring (MRM) models. The method gave good linear relations with regression coefficients ranging from 0.9950 to 0.9999. The detection limits ranged from 0.03 to 15.0 µg·kg−1, and the average recovery at three different concentrations ranged from 60.2% to 115.8%, with relative standard deviations (RSD%) varying from 0.7% to 19.6% for the 25 mycotoxins. The method is simple, rapid, accurate, and an improvement compared with the existing methods published so far.

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“…Clean-up is also performed by solid-phase extraction through the addition of appropriate sorbents. Although, the QuEChERS method was initially developed and subsequently modified for pesticide analysis in fruits and vegetables, it has been recently applied for detection of mycotoxins successfully (Azaiez, Giusti, Sagratini, Mañes, & Fernández-Franzón, 2014;Dzuman, Zachariasova, Lacina, & Veprikova, 2014;Koesukwiwat, Sanguankaew, & Leepipatpiboon, 2014;Vaclavik, Zachariasova, Hrbek, & Hajslova, 2010). However, it has not been studied for the extraction of Alternaria toxins from pomegranates.…”

Section: Introductionmentioning

confidence: 99%

“…Thin-layer chromatography (TLC), gas chromatography (GC), and high performance liquid chromatography (HPLC) are the techniques usually used for the determination of Alternaria toxins. GC analysis requires a derivatisation step proving the HPLC technique more suitable for the analysis of polar compounds like mycotoxins (Andersen et al, 2006;Azaiez et al, 2014;Delgado, Gómez-Cordovés, & Scott, 1996;Dzuman et al, 2014;Koesukwiwat et al, 2014;Pavón, Luna, de la Cruz, González, & Martín, 2012;Vaclavik et al, 2010). Detection of separated products can be achieved with fluorescence detection (FLD), UV detection, diode array detection (DAD), single mass spectrometry (MS) or tandem MS (MS/MS) (EFSA, 2011;Ostry, 2008).…”

Section: Introductionmentioning

confidence: 99%

Determination of mycotoxins in pomegranate fruits and juices using a QuEChERS-based method

et al. 2015

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Multi-mycotoxins Analysis in Dried Fruit by LC/MS/MS and a Modified QuEChERS Procedure

Cited by 69 publications

References 27 publications

Quantification of mycotoxins in vegetable oil by UPLC-MS/MS after magnetic solid-phase extraction

Quantification of mycotoxins in vegetable oil by UPLC-MS/MS after magnetic solid-phase extraction

QuEChERS Purification Combined with Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry for Simultaneous Quantification of 25 Mycotoxins in Cereals

Determination of mycotoxins in pomegranate fruits and juices using a QuEChERS-based method

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