Multi-omics analysis reveals the impact of intratumor microbes on lung adenocarcinoma prognosis and ICB treatment

Abstract

Recent studies indicated that the presence of microorganisms within tumors constitutes a pivotal component of the tumor microenvironment (TME).In this study, we conducted a comprehensive analysis of intratumoral microbial factors employing multi-omics methodologies to elucidate the potential determinants influencing immune cell infiltration (ICI) and the pathogenesis of pulmonary adenocarcinoma (LUAD).We ascertained the constitution and configuration of intratumoral microbial communities as significant contributors to the disparities in survival rates observed among distinct ICI subtypes.Various immune cells infiltrating tumors,along with immunoregulatory genes,exhibited associations with the abundance of intratumoral microorganisms, consequently exerting indirect effects on immune checkpoint blockade (ICB) therapy.Our analysis underscores the critical role of intratumoral microorganisms in modulating the prognosis of ICI patterns and therapeutic responses in LUAD, thereby offering insightful guidance for refining contemporary immunotherapeutic strategies and prognostication for LUAD patients. importance:Using the multi-omics data from the Cancer Genome Atlas (TCGA) LUAD cohort,we conducted a comprehensive assessment of the patterns of TME infiltration and unravelled the intricate interplay between gene expression and intratumoral microorganisms.This study highlights the significant impact of intratumoral microorganisms in promoting the progression of LUAD by influencing the tumor immune microenvironment and modulating responses to ICB therapy.Through meticulous analysis,we identified a constellation of composite features,encompassing four ICB-associated tumor-infiltrating immune cells(TIICs) and eighteen microorganisms of pertinence.