Multi-omics reveals that green pea (<i>Pisum sativum</i> L.) hull supplementation ameliorates non-alcoholic fatty liver disease <i>via</i> the SHMT2/glycine/mTOR/PPAR-γ signaling pathway

Guo, Fanghua; Xiong, Hua; Tsao, Rong; Wen, Xiang-Yang; Liu, Jiahua; Chen, Dongying; Jiang, Li; Sun, Yong

doi:10.1039/d3fo01771k

Cited by 4 publications

(6 citation statements)

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“…Serum d -mannitol levels were measured to reflect intestinal permeability . The experimental operation were based on our previous method . Briefly, after administration of d -mannitol for 6 h, blood was obtained for determination of d -mannitol concentration by an ELISA kit.…”

Section: Methodsmentioning

confidence: 99%

“…The metabolomics experiment was operated according to our previous method . Briefly, the metabolites in liver tissue were extracted with methanol:acetonitrile:water (2:2:1, v/v/v), and the supernatant was collected for LC-MS analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, the metabolites in liver tissue were extracted with methanol:acetonitrile:water (2:2:1, v/v/v), and the supernatant was collected for LC-MS analysis. LC and MS conditions were the same as our previous study . Raw data was analyzed with BMDB (BGI Co., Ltd., China), mzCloud (), and Chemspider () databases.…”

Section: Methodsmentioning

confidence: 99%

“…The transcriptomic experiment was operated according to our previous method . Briefly, the total RNA extracted from liver tissue was first tested for concentration and purity and then sequenced on the DNBSEQ platform.…”

Section: Methodsmentioning

confidence: 99%

“…The OGTT experiment was operated based on our previous method . Briefly, the mice were orally administered glucose solution, and then blood glucose concentration was detected at 0, 15, 30, 60, 90, and 120 min.…”

Section: Methodsmentioning

confidence: 99%

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Green Pea (Pisum sativum L.) Hull Polyphenol Extract Alleviates NAFLD through VB6/TLR4/NF-κB and PPAR Pathways

Guo,

Xiong,

Tsao

et al. 2023

J. Agric. Food Chem.

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Green pea hull is a processing byproduct of green pea and rich in polyphenols. Nonalcoholic fatty liver disease (NAFLD) is a chronic metabolic disease characterized by accumulation of lipids in the liver for which there are no effective treatment strategies. Here, a mouse model of NAFLD induced by a DSS+high-fat diet (HFD) was established to investigate the effect of green pea hull polyphenol extract (EGPH). The results show that EGPH relief of NAFLD was a combined effect, including reducing hepatic fat accumulation, improving antioxidant activity and blood lipid metabolism, and maintaining glucose homeostasis. Increased intestinal permeability aggravated NAFLD. Combined metabolomics and transcriptomic analysis showed that vitamin B6 is the key target substance for EGPH to alleviate NAFLD, and it may be the intestinal flora metabolite. After EGPH intervention, the level of vitamin B6 in mice was significantly increased, and more than 60% in the blood enters the liver, which activated or inhibited PPAR and TLR4/NF-κB signaling pathways to relieve NAFLD. Our research could be a win−win for expanding the use of green pea hull and the search for NAFLD prophylactic drugs.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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