Multi-Omics Strategies for Decoding Smoke-Assisted Germination Pathways and Seed Vigour

Bose, Utpal; Juhász, Angéla; Broadbent, James A.; Komatsu, Setsuko; Colgrave, Michelle L.

doi:10.3390/ijms21207512

Cited by 13 publications

(8 citation statements)

References 116 publications

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“…Recent studies have reported that exogenous IAA application higher than 1 μM determines the inhibition or delay of seed germination in plants [ 33 , 34 ]. However, the mechanism of auxin action in seed germination has not been completely elucidated, although there are studies which identified the key role of interactions between IAA, abscisic acid (ABA) and even gibberellic acid (GA) to control the seed dormancy and germination [ 35 , 36 ].…”

Section: Resultsmentioning

confidence: 99%

Bioprospecting Fluorescent Plant Growth Regulators from Arabidopsis to Vegetable Crops

Halip

Maffei

Croitor

et al. 2021

IJMS

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The phytohormone auxin is involved in almost every process of a plant’s life, from germination to plant development. Nowadays, auxin research connects synthetic chemistry, plant biology and computational chemistry in order to develop innovative and safe compounds to be used in sustainable agricultural practice. In this framework, we developed new fluorescent compounds, ethanolammonium p-aminobenzoate (HEA-pABA) and p-nitrobenzoate (HEA-pNBA), and investigated their auxin-like behavior on two main commercial vegetables cultivated in Europe, cucumber (Cucumis sativus) and tomato (Solanumlycopersicum), in comparison to the model plant Arabidopsis (Arabidopsis thaliana). Moreover, the binding modes and affinities of two organic salts in relation to the natural auxin indole-3-acetic acid (IAA) into TIR1 auxin receptor were investigated by computational approaches (homology modeling and molecular docking). Both experimental and theoretical results highlight HEA-pABA as a fluorescent compound with auxin-like activity both in Arabidopsis and the commercial cucumber and tomato. Therefore, alkanolammonium benzoates have a great potential as promising sustainable plant growth stimulators to be efficiently used in vegetable crops.

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Section: Resultsmentioning

confidence: 99%

Bioprospecting Fluorescent Plant Growth Regulators from Arabidopsis to Vegetable Crops

Halip

Maffei

Croitor

et al. 2021

IJMS

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“…Karrikin, which is one component of the plant-derived smoke solution [ 18 ], regulated tolerance to abiotic stresses such as drought [ 19 ] and cold [ 20 ] in Arabidopsis thaliana as well as salt [ 21 ] and cadmium [ 22 ] in oil plant. Furthermore, it was reported that the plant-derived smoke solution enhanced soybean growth under flooding [ 13 ] and after flooding [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…Butanolides, including karrikins and cyanohydrin, are the active compounds in the plant-derived smoke solution [ 6 ]. The functional mechanisms of plant-derived smoke in the seed-germination stage were clarified with the discovery of karrikin [ 18 ]. A study on the molecular aspects of seed germination reported that abscisic acid, seed maturation, and dormancy-related transcripts were up-regulated by trimethyl butenolide and suppressed by karrikin 1, indicating that increased seed germination by karrikin 1 might be due to suppression of abscisic acid [ 6 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Morphological, Biochemical, and Proteomic Analyses to Understand the Promotive Effects of Plant-Derived Smoke Solution on Wheat Growth under Flooding Stress

Komatsu

Yamaguchi

Hitachi

et al. 2022

Plants

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Wheat is an important staple food crop for one-third of the global population; however, its growth is reduced by flooding. On the other hand, a plant-derived smoke solution enhances plant growth; however, its mechanism is not fully understood. To reveal the effects of the plant-derived smoke solution on wheat under flooding, morphological, biochemical, and proteomic analyses were conducted. The plant-derived smoke solution improved wheat-leaf growth, even under flooding. According to the functional categorization of proteomic results, oppositely changed proteins were correlated with photosynthesis, glycolysis, biotic stress, and amino-acid metabolism with or without the plant-derived smoke solution under flooding. Immunoblot analysis confirmed that RuBisCO activase and RuBisCO large/small subunits, which decreased under flooding, were recovered by the application of the plant-derived smoke solution. Furthermore, the contents of chlorophylls a and b significantly decreased by flooding stress; however, they were recovered by the application of the plant-derived smoke solution. In glycolysis, fructose-bisphosphate aldolase and glyceraldehyde-3-phosphate dehydrogenase decreased with the application of the plant-derived smoke solution under flooding as compared with flooding alone. Additionally, glutamine, glutamic acid, aspartic acid, and serine decreased under flooding; however, they were recovered by the plant-derived smoke solution. These results suggest that the application of the plant-derived smoke solution improves the recovery of wheat growth through the regulation of photosynthesis and glycolysis even under flooding conditions. Furthermore, the plant-derived smoke solution might promote wheat tolerance against flooding stress through the regulation of amino-acid metabolism.

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“…S1, Supplementary material). Recent studies have reported that exogenous IAA application higher than 1μM determines the inhibition or delay of seed germination in plants [31,32]. However, the mechanism of auxin action in seed germination has not been completely elucidated, although there are studies which identified the key role of interactions between IAA, abscisic acid (ABA) and even gibberellic acid (GA) to control the seed dormancy and germination [33,34].…”

Section: Bioactivity Assaysmentioning

confidence: 99%

Bioprospecting Fluorescent Plant Growth Regulators from Arabidopsis to Vegetable Crops

Halip¹,

Maffei²,

Croitor³

et al. 2021

Preprint

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The phytohormone auxin is involved in almost every process of a plant’s life, from germination to plant development. Nowadays, auxin research connects synthetic chemistry, plant biology, and computational chemistry in order to develop innovative and safe compounds to be used in sustainable agricultural practice. In this framework, we developed new environmentally-friendly fluorescent compounds, ethanolammonium p-aminobenzoate (HEA-pABA) and p-nitrobenzoate (HEA-pNBA), and investigated their auxin-like behavior on two main commercial vegetables cultivated in Europe, cucumber (Cucumis sativus) and tomato (Solanum lycopersicum), in comparison to the model plant Arabidopsis (Arabidopsis thaliana). Moreover, the binding modes and affinities of two organic salts in relation to the natural auxin indole-3-acetic acid (IAA) into TIR1 auxin receptor were investigated by computational approaches (homology modeling and molecular docking). Both experimental and theoretical results highlight HEA-pABA as a fluorescent compound with auxin-like activity both in Arabidopsis and the commercial cucumber and tomato. Therefore, alkanolammonium benzoates have a great potential as promising sustainable plant growth stimulators to be efficiently used in vegetable crops.

show abstract

Multi-Omics Strategies for Decoding Smoke-Assisted Germination Pathways and Seed Vigour

Cited by 13 publications

References 116 publications

Bioprospecting Fluorescent Plant Growth Regulators from Arabidopsis to Vegetable Crops

Bioprospecting Fluorescent Plant Growth Regulators from Arabidopsis to Vegetable Crops

Morphological, Biochemical, and Proteomic Analyses to Understand the Promotive Effects of Plant-Derived Smoke Solution on Wheat Growth under Flooding Stress

Bioprospecting Fluorescent Plant Growth Regulators from Arabidopsis to Vegetable Crops

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